[1]林俊,陈冰,覃绍敏,等.广西伪狂犬病毒流行株的分离鉴定及遗传变异分析[J].江苏农业学报,2016,(01):142-150.[doi:10.3969/j.issn.1000-4440.2016.01.022 ]
　LIN Jun,CHEN Bing,QIN Shao-min,et al.Identification and genetic variation of pseudorabies virus in Guangxi province[J].,2016,(01):142-150.[doi:10.3969/j.issn.1000-4440.2016.01.022 ]
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广西伪狂犬病毒流行株的分离鉴定及遗传变异分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年01期
页码:
142-150
栏目:
畜牧兽医·水产养殖
出版日期:
2016-01-08

文章信息/Info

Title:
Identification and genetic variation of pseudorabies virus in Guangxi province
作者:
林俊1; 陈冰1; 覃绍敏1; 曹颖颖1; 刘金凤1; 石永胜2; 白安斌1; 赵武1;  吴健敏1
（1.广西壮族自治区兽医研究所，广西南宁530001；2.北海市动物疫病预防控制中心，广西北海536000）
Author(s):
LIN Jun1; CHEN Bing1; QIN Shao-min1; CAO Ying-ying1; LIU Jin-feng1; SHI Yong-sheng2; 〖JP〗BAI An-bin1;  ZHAO Wu1; WU Jian-min1
(1.Guangxi Veterinary Research Institute,Nanning 530001,China;2.Beihai Center for Animal Disease Control and Prevention,Beihai 536000,China)
关键词:
伪狂犬病毒; 分离鉴定; gE基因; gC基因; 遗传变异分析
Keywords:
pseudorabies virus; isolation and identification; gE gene; gC gene; genetic analysis
分类号:
S852.65
DOI:
10.3969/j.issn.1000-4440.2016.01.022
文献标志码:
A
摘要:
为了更好防控广西伪狂犬病，本研究采集广西北海发生疑似伪狂犬病疫情（AD）猪场的犬和猪样品进行病毒分离，通过动物接种试验、电镜观察及核酸检测，证实分离到2株PRV毒株。2毒株与GenBank上登录的参考毒株gE和gC基因核苷酸序列同源性为97.4%～100.0%和91.3%～99.8%；氨基酸序列同源性为95.1%～100.0%和86.6%～99.5%。基于gE基因和gC基因的进化分析显示，2株分离毒与国内不同时期的毒株在进化树上共同构成一个进化分支，与欧洲和美洲的毒株亲缘关系较远。对氨基酸序列的分析结果显示，2株分离毒的gE基因决定毒力的关键位点未发生突变。基于gE蛋白质与gC蛋白质的分析结果显示，虽然与国外毒株（Rice和Bartha）相比存在多个氨基酸位点的变异，但与国内的分离毒，尤其是2011年后的流行毒株，变异情况基本一致。
Abstract:
Two pseudorabies viruses（PRV） were isolated from the dead canines and pigs in a Beihai farm of Guangxi.The two types of animals were suspicious of dying of Aujeszky′s disease(AD). The isolates were detected by animal infection test, observed by transmission electron microscope observation and analyzed by PCR.The two isolates shared homologies of 97.4%-100.0% with gE and 91.3%-99.8% with gC in nucleotide sequence on GenBank,and 95.1%-100.0% with gE and 86.6%-99.5% with gC in amino acid sequnence.The isolates and other Chinese isolates were classified into the same clade and showed distant relation with foreign isolates,based on the genetic analysis of gE gene and gC gene. Two isolates did not show mutation in the key site of amino acid. Compared to foreign strains, the amino acid sequences of the two isolates presented multiple mutations, however, the mutations were similar to those in Chinas strains especially those isolated after year 2011.

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备注/Memo

备注/Memo:
收稿日期：2015-06-30 基金项目： 国家农业科技成果转化基金项目（2013GB2E100373）；广西自然基金项目（2011GXNSFA018086）；广西基本科研业务费专项（桂科专项13-4） 作者简介：林俊(1986-)，男，广西南宁人，硕士，研究实习员，主要从事动物传染病与分子免疫学研究。（Tel）0771-3120373；（Email）spirit1899@hotmail.com
更新日期/Last Update: 2017-05-08