[1]刘永惠,沈一,陈志德.花生干旱及高盐胁迫下的酵母双杂交文库构建[J].江苏农业学报,2016,(01):40-43.[doi:10.3969/j.issn.1000-4440.2016.01.006 ]
 LIU Yong-hui,SHEN Yi,CHEN Zhi-de.Construction and identification of a yeast two-hybrid cDNA library of peanut under drought and high salinity stress[J].,2016,(01):40-43.[doi:10.3969/j.issn.1000-4440.2016.01.006 ]
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花生干旱及高盐胁迫下的酵母双杂交文库构建()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年01期
页码:
40-43
栏目:
遗传育种·生理生化
出版日期:
2016-01-08

文章信息/Info

Title:
Construction and identification of a yeast two-hybrid cDNA library of peanut under drought and high salinity stress
作者:
刘永惠沈一陈志德
(江苏省农业科学院经济作物研究所,江苏南京210014)
Author(s):
LIU Yong-huiSHEN YiCHEN Zhi-de
(Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
花生干旱高盐酵母双杂交cDNA文库
Keywords:
peanutdroughthigh salinitya yeast two-hybrid cDNA library
分类号:
S565.2
DOI:
10.3969/j.issn.1000-4440.2016.01.006
文献标志码:
A
摘要:
干旱、高盐等胁迫对花生的产量和品质影响较大,为了挖掘花生耐干旱和高盐胁迫相关基因,并阐释其编码蛋白质间的互作关系,本研究利用SMART技术,构建了花生干旱及高盐胁迫下的酵母双杂交文库。结果表明,构建的文库容量为 1.26×106 CFU,文库滴度达到 1.07×108CFU/ml,从随机挑选的16个克隆的PCR结果来看,插入片段大小差异明显,主要分布在 500~2 000 bp,重组率为100%。说明该文库具有较高的质量,可以用于进一步的筛选工作。
Abstract:
Yield and quality of peanut (Arachis hypogaea L.)

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2015-08-15 基金项目:国家现代农业花生产业技术体系项目(CARS-14);江苏省农业科技自主创新基金项目[CX(14)2001] 作者简介:刘永惠(1985-),女,江苏盐城人,硕士,助理研究员,主要从事花生资源研究工作。(Tel)13770633970 通讯作者:陈志德,(Tel)13851834013
更新日期/Last Update: 2017-05-08