[1]陈飞,李蕾,周佳宇,等.茅苍术挥发油及主要组分对3种病原菌和2种内生菌的抑菌活性[J].江苏农业学报,2015,(06):1270-1277.[doi:doi:10.3969/j.issn.1000-4440.2015.06.012]
　CHEN Fei,LI Lei,ZHOU Jia-yu,et al.Antifungal activities of essential oil from Atractylis lancea against three species of pathogenic fungi and two species of endophytic fungi[J].,2015,(06):1270-1277.[doi:doi:10.3969/j.issn.1000-4440.2015.06.012]
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茅苍术挥发油及主要组分对3种病原菌和2种内生菌的抑菌活性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年06期
页码:
1270-1277
栏目:
植物保护
出版日期:
2015-12-31

文章信息/Info

Title:
Antifungal activities of essential oil from Atractylis lancea against three species of pathogenic fungi and two species of endophytic fungi
作者:
陈飞; 李蕾; 周佳宇; 戴传超
(南京师范大学生命科学学院/江苏省微生物资源产业化工程技术研究中心/江苏省微生物与功能基因组学重点实验室，江苏南京210023)
Author(s):
CHEN Fei; LI Lei; ZHOU Jia-yu; DAI Chuan-chao
(Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources/Jiangsu Key Laboratory for Microbes and Functional Genomics/College of Life Science，Nanjing Normal University, Nanjing 210023, China)
关键词:
茅苍术; 挥发油; 病原真菌; 内生真菌; 抑菌活性
Keywords:
Atractylis lancea; volatile oil; pathogenic fungus; endophytic fungus; antifungal activity
分类号:
S432.2； 567.21+1
DOI:
doi:10.3969/j.issn.1000-4440.2015.06.012
文献标志码:
A
摘要:
研究内生真菌如何与宿主植物建立拮抗平衡，以及该过程与病原菌侵染过程的差别。以药用植物茅苍术及几种真菌为材料，采用水蒸馏法提取挥发油并分离各组分，应用平板生长速率法、滤纸片扩散法、悬滴法和扫描电镜观察研究挥发油对病原真菌和内生真菌的抑菌作用。结果表明，高浓度挥发油对病原菌中交链孢的抑菌活性最强，达77.56%，对尖孢镰刀菌和腐皮镰刀菌的抑菌作用次之，分别为71.75% 和70.07%，而对2种内生真菌AL11和AL12则具有较小抑制作用，分别为61.90% 和23.57%；挥发油中β-桉叶醇和茅术醇对5种真菌均无抑制作用，苍术素相比于苍术酮对病原菌有更强的抑制效果；在苍术酮和苍术素的胁迫下，病原真菌的孢子萌发受到了不同程度的抑制，内生真菌表现为菌丝分支增多，分支间距离变短；苍术酮和苍术素可以引起交链孢的菌丝断裂，褶皱，细胞表面疣状突起。说明茅苍术挥发油及其主要组分苍术酮和苍术素对内生真菌生长有限制作用，但这种作用明显低于对病原菌的抑制作用，说明苍术酮和苍术素很可能是宿主植物与内生真菌建立拮抗平衡的关键物质。
Abstract:
This study aims to investigate how the endophytic fungi and host plant establish an antagonistic balance and its difference with pathogen infection. The volatile oil and four primary components from medicinal plant Atractylodes lancea were extracted by water distillation extraction, and its antifungal activities against three species of pathogenic fungi and two species of endophytic fungi were determined by plate growth method, paper disc assay, drop method and scanning electron microscopy. The volatile oil exhibited the strongest antifungal activity of 77.56% against Alternaria solani, stronger activities against Fusarium solani and F. oxysporum, and weak activities against two species of endophytic fungi (61.90% and 23.57%). Volatile oil components β-eudesmol and atractylol had no inhibitory effect on five species of fungi, and atractydin has stronger inhibitory activity than atractylon against pathogenic fungi. Under the stress of atractydin and atractylon, the sporulation of pathogenic fungus was suppressed in different extent, the hyphal branches of endophytic fungus increased, and the distance between the branches became shorter. The volatile oil and its main components atractydin and atractylon exhibited weaker inhibitory activity against endophytic fungi than against pathogenic fungi, indicating that atractydin and atractylon might be the key metabolites to balance endophytic fungi and host plant.

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备注/Memo

备注/Memo:
收稿日期：2015-07-01 基金项目：国家自然科学基金项目(21406119、31070443)；江苏省高校自然科学基金项目(14KJB180009) 作者简介：陈飞(1982-)，男，河南汝南人，博士，讲师，研究方向为微生物与植物互作及其在药用植物次级代谢调控的应用。(Tel) 025-85891382; (E-mail) chenfei@njnu.edu.cn。李蕾为共同第一作者。 通讯作者：戴传超，(Tel) 025-85891382; (E-mail) daichuanchao@njnu.edu.cn
更新日期/Last Update: 2015-12-31