[1]俞洁琼,王洪成,何正文,等.红色亚栖热菌耐热木聚糖酶的克隆表达及生物信息学分析[J].江苏农业学报,2015,(06):1242-1249.[doi:doi:10.3969/j.issn.1000-4440.2015.06.008]
　YU Jie-qiong,WANG Hong-cheng,HE Zheng-wen,et al.Gene cloning, expression and bioinformatics analysis of a novel xylanase from Meiothermus ruber[J].,2015,(06):1242-1249.[doi:doi:10.3969/j.issn.1000-4440.2015.06.008]
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红色亚栖热菌耐热木聚糖酶的克隆表达及生物信息学分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年06期
页码:
1242-1249
栏目:
遗传育种·生理生化
出版日期:
2015-12-31

文章信息/Info

Title:
Gene cloning, expression and bioinformatics analysis of a novel xylanase from Meiothermus ruber
作者:
俞洁琼; 王洪成; 何正文; 乐易林; 邵蔚蓝
（江苏大学环境与安全工程学院/生物质能源研究所，江苏镇江212013）
Author(s):
YU Jie-qiong; WANG Hong-cheng; HE Zheng-wen; LE Yi-lin; SHAO Wei-lan
(Biofuels Institute/School of the Environment and Safety Engineering, Jiangsu University， Zhenjiang 212013, China)
关键词:
红色亚栖热菌; 木聚糖酶; 生物信息学分析; 高温酶
Keywords:
Meiothermus ruber; xylanase; bioinformatics analysis; thermophic enzyme
分类号:
S943.2
DOI:
doi:10.3969/j.issn.1000-4440.2015.06.008
文献标志码:
A
摘要:
从自主分离的Meiothermus. rubber菌株的基因组中克隆到木聚糖酶基因，并进行了超量表达、重组酶的纯化和酶学性质研究。结果表明，重组木聚糖酶Mru是热稳定性酶，最适反应温度为65 ℃，能够有效降解木聚糖产生木糖和木二糖。生物信息学分析发现，木聚糖酶Mru与数据库中来自亚栖热菌的木聚糖酶的同源性为 60%～100%。在木聚糖酶Mru的结构模型中，GH10木聚糖酶的保守位点大多位于β折叠，α螺旋保守性很低。本研究揭示，木聚糖酶Mru代表一类新型热稳定性GH10木聚糖酶。
Abstract:
This paper reports a novel GH10 xylanase produced by thermophilic bacterium Meiothermus rubber. The xylanase gene was cloned from the genomic DNA of a new isolate of M. rubber, and overexpressed in Escherichia coli, and recombinant Mru xylanase was purified and characterized. Mru xylanase efficiently released xylose from oat spelt xylan at 55-75 ℃ with the highest activity at 65 ℃. The bioinformatics analysis indicated that Mru xylanase shared ≥61% similarities with the xylanases of Meiothermus spp., but ≤49% similarities with the other GH10 xylanases in database. The prediction of the Mru xylanase structure revealed that the conserved sequences for GH10 xylanases mainly occurred in the β-sheets and few identities were observed in α-helixes. Mru xylanase represents a type of heat-stable G10 xylanase.

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备注/Memo

备注/Memo:
收稿日期：2015-05-05 基金项目：国家自然科学基金项目(31170027、31300088) 作者简介：俞洁琼(1989-)，女，江西上饶人，硕士研究生，主要从事分子生物学研究。 通讯作者：邵蔚蓝，(Tel)13913856528；(E-mail)weilanshao@foxmail.com
更新日期/Last Update: 2015-12-31