[1]李隐侠,乔永,张俊,等.湖羊肌肉UCP3基因序列特征分析及其在肌肉中的表达[J].江苏农业学报,2015,(05):1078-1083.[doi:doi:10.3969/j.issn.1000-4440.2015.05.021]
　LI Yin-xia,QIAO Yong,ZHANG Jun,et al.Characterization of UCP3 gene sequence and its expression in Hu lamb muscles[J].,2015,(05):1078-1083.[doi:doi:10.3969/j.issn.1000-4440.2015.05.021]
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湖羊肌肉UCP3基因序列特征分析及其在肌肉中的表达()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年05期
页码:
1078-1083
栏目:
畜牧兽医·水产养殖
出版日期:
2015-10-31

文章信息/Info

Title:
Characterization of UCP3 gene sequence and its expression in Hu lamb muscles
作者:
李隐侠12; 乔永3; 张俊12; 钱勇12; 孟春花1; 王慧利12; 钟声12; 曹少先12; 李齐发3
（1.江苏省农业科学院畜牧研究所，江苏南京210014;2.江苏省农业科学院动物品种改良和繁育重点实验室，江苏南京210014；3.南京农业大学动物科技学院，江苏南京210095）
Author(s):
LI Yin-xia12; QIAO Yong3; ZHANG Jun12; QIAN Yong12; MENG Chun-hua12; WANG Hui-li12; ZHONG Sheng12; CAO Shao-xian12; LI Qi-fa3
（1.Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China；2.Key laboratory of Animal Breeding and Reproduction/Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;3.Academy of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China）
关键词:
UCP3; 序列特征; 系统进化; real-time PCR; 湖羊
Keywords:
UCP3; sequence characterization; phylogenetic evolution; real-time PCR; Hu sheep
分类号:
S826.8+9
DOI:
doi:10.3969/j.issn.1000-4440.2015.05.021
文献标志码:
A
摘要:
为了获得湖羊UCP3基因编码区全序列并分析其序列特征，同时研究其在湖羊不同部位肌肉中的表达变化，选取初生与1、2、3、4、5和6月龄湖羊公羔各5只，提取屠宰后背最长肌、腰大肌、前腿肱二头肌和后腿股二头肌RNA，用TA克隆技术获得湖羊UCP3基因编码区序列，用DNAMAN软件分析UCP3基因在哺乳动物间的保守性，同时基于UCP3蛋白质序列用MEGA5.1软件构建系统发育树，用Real-time PCR检测湖羊不同肌肉部位UCP3基因的表达水平并分析其在不同发育时期的变化。结果显示：湖羊UCP3基因编码区全长936 bp，编码311个氨基酸残基，在哺乳动物中相对保守。根据UCP3蛋白质氨基酸序列构建的系统发育树显示湖羊与牛的亲缘关系最近。Real-time PCR结果显示，初生至6月龄的湖羊不同部位肌肉UCP3基因的表达模式基本相似，即在出生时有较低的水平，然后上升，随着月龄的增加又下降,但UCP3基因在后腿股二头肌中的表达量整体偏低，在腰大肌中表达水平变化较大，3月龄的表达水平显著高于初生和5月龄。
Abstract:
This study aims to obtain the full coding region of UCP3 gene in Hu sheep, characterize its sequence and investigate the mRNA level in sheep muscles. Each five male Hu lamb at 0 to 6 months ages were chosen and slaughtered, and total RNA from longissimus muscles, psoas muscles and front and rear leg biceps femoris muscles were extracted. The coding region of UCP3 gene of Hu sheep was cloned using TA cloning technoloty, and its conservation among different mammals was analyzed using DNAMAN software. Its mRNA level of different muscles of Hu sheep was investigated using real time PCR. The results showed that the coding region of UCP3 gene in Hu sheep was 936 bp，encoding 311 amino acid residues, and was relatively conserved in mammals. Phylogenetic tree constructed based on protein sequence of UCP3 showed that the genetic relationship between Hu sheep and cattle was the closest. Real-time PCR results revealed that UCP3 in different parts of muscles of differently-aged Hu sheep shared similar mRNA expression patterns, which were low at birth, and went up slightly and down afterwards over age. The expression level of UCP3 gene was relatively low in the rear leg biceps femoris muscles, and fluctuated in psoas muscles. UCP3 gene showed higher levels in 3-mouth-old sheep than those in the newborns and 5-mouth-old sheep.〖JP〗

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备注/Memo

备注/Memo:
收稿日期：2015-03-03基金项目：江苏省农业科技自主创新基金项目[CX(15)1007]；江苏省自然科学基金项目（BK20140750）；科技副总（企业创新岗）项目；苏州市吴中区农业科技计划项目（WN201301）作者简介：李隐侠(1979-)，女，河南固始人，博士，助理研究员，主要从事动物遗传育种与繁殖方面的研究。（Tel）025-84390095;(Email)liyxmh@126.com
更新日期/Last Update: 2015-10-31