[1]谢珊珊,黄金,杨发龙.绵羊肺炎支原体P109蛋白质分子特征、原核表达及其免疫反应性[J].江苏农业学报,2015,(04):824-828.[doi:10.3969/j.issn.1000-4440.2015.04.018]
　XIE Shan-shan,HUANG Jin,YANG Fa-long.Molecular characterization, prokaryotic expression and immunoreactivity of Mycoplasma ovipneumoniae P109 protein[J].,2015,(04):824-828.[doi:10.3969/j.issn.1000-4440.2015.04.018]
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绵羊肺炎支原体P109蛋白质分子特征、原核表达及其免疫反应性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年04期
页码:
824-828
栏目:
畜牧兽医·水产养殖
出版日期:
2015-08-31

文章信息/Info

Title:
Molecular characterization, prokaryotic expression and immunoreactivity of Mycoplasma ovipneumoniae P109 protein
作者:
谢珊珊; 黄金; 杨发龙
（西南民族大学生命科学与技术学院/动物医学四川省高等学校重点实验室，四川成都610041）
Author(s):
XIE Shan-shan; HUANG Jin; YANG Fa-long
(College of Life Science and Technology, Southwest University for Nationalities/Key Laboratory of Veterinary Medicine of Unirersity in Sichuan Province, Chengdu 610041, China)
关键词:
绵羊肺炎支原体; P109蛋白质; 原核表达; 免疫反应性
Keywords:
Mycoplasma ovipneumoniae; P109 protein; prokaryotic expression; immunoreactivity
分类号:
S826
DOI:
10.3969/j.issn.1000-4440.2015.04.018
文献标志码:
A
摘要:
为了研究绵羊肺炎支原体P109蛋白质的结构与功能，本试验对p109基因进行生物信息学分析，并对其部分基因片段进行了PCR扩增、克隆和原核表达，采用Western-blot方法对其免疫反应性进行分析。结果显示，P109基因与猪肺炎支原体黏附相关基因mhp384高度同源。重组蛋白质在大肠杆菌Rosetta(DE3)工程菌中成功获得表达，以包涵体的形式存在；经纯化的重组蛋白质与山羊抗绵羊肺炎支原体全菌血清发生结合反应，表明P109蛋白质是绵羊肺炎支原体的免疫原之一。
Abstract:
To study the structure and function of P109 protein of Mycoplasma ovipneumoniae, bioinformatics analysis were performed and a partial fragment of p109 was cloned and expressed in Escherichia coli. Immunoreactivity of P109 was analyzed by Western-blot. P109 was highly homologous to M. hyopneumoniae mhp384, an adhesion-related gene. The recombinant protein was successfully expressed in Escherichia coli Rosetta(DE3) as inclusion body. The purified recombinant protein reacted with goat serum against M. ovipneumoniae, suggesting protein P109 was one of the immunogens of M. ovipneumoniae.

参考文献/References:


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备注/Memo

备注/Memo:
收稿日期：2015-04-27 基金项目：国家自然科学基金项目(31272588); 四川省杰出青年学术技术带头人培育计划项目(2013JQ0040) 作者简介：谢珊珊(1992-),女,安徽安庆人,本科，主要从事生物技术研究。（Tel）17755647950；（E-mail） 1035724831@qq.com 通讯作者：杨发龙，（E-mail）yfalong@hotmail.com
更新日期/Last Update: 2015-08-31