[1]王金玲,王永,刘鲁蜀,等.草地藏系绵羊生长激素释放激素基因部分cDNA的克隆及生物信息学分析[J].江苏农业学报,2015,(03):595-599.[doi:10.3969/j.issn.1000-4440.2015.03.021]
　WANG Jin-ling,WANG Yong,LIU Lu-shu,et al.Cloning and bioinformatics analysis of a fragment cDNA encoding growth hormone-releasing hormone in Tibetan sheep[J].,2015,(03):595-599.[doi:10.3969/j.issn.1000-4440.2015.03.021]
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草地藏系绵羊生长激素释放激素基因部分cDNA的克隆及生物信息学分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年03期
页码:
595-599
栏目:
畜牧兽医·水产养殖
出版日期:
2015-06-30

文章信息/Info

Title:
Cloning and bioinformatics analysis of a fragment cDNA encoding growth hormone-releasing hormone in Tibetan sheep
作者:
王金玲1; 王永2; 刘鲁蜀3; 陶永平4
(1.绵阳师范学院，四川绵阳621000；2.西南民族大学青藏高原动物遗传资源保护与利用四川省重点实验室，四川成都610041；3.西南民族大学 生命科学与技术学院，四川成都610041；4.四川省若尔盖县畜牧局，四川若尔盖624500)
Author(s):
WANG Jin-ling1; WANG Yong2; LIU Lu-shu3; TAO Yong-ping4
(1.Mianyang Normal University, Mianyang 621000, China;2.Key Laboratory for The Qing-Tibet Plateau Animal Genetic Resource Conservation and Exploitation of Sichuan Province, South West University of Nationalities, Chengdu 610041, China;3.College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China;4.Ruoergai Bureau of Agriculture and Animal Husbandry, Ruoergai 624500, China)
关键词:
藏系绵羊; GHRH; 基因克隆
Keywords:
tibetan sheep; GHRH; gene clone
分类号:
Q959.8
DOI:
10.3969/j.issn.1000-4440.2015.03.021
文献标志码:
A
摘要:
为了克隆草地藏系绵羊生长激素释放激素基因，采用Trizol法从草地藏系绵羊下丘脑组织中提取总RNA，用反转录-聚合酶链式反应（RT-PCR）进行cDNA扩增并克隆测序，获得长度为207 bp的促生长激素释放激素基因（GHRH）的部分cDNA序列。结果表明获得的草地藏系绵羊GHRH部分cDNA序列与GenBank中注册的绵羊GHRH基因编码起始位置（86位）到292位区域高度同源，仅有1个碱基的差异，该cDNA序列编码69个氨基酸残基，其内含有信号肽序列，该氨基酸序列的 31～57位具有典型胰高血糖素类似激素特征的GLUCA结构域。
Abstract:
Total RNA was extracted from the hypothalamus tissues of Tibetan sheep using Trizol method and cDNA was amplified by reverse transcription polymerase chain reaction (RT-PCR). The cDNA of Tibetan sheep GHRH gene was cloned from the amplified PCR product and sequenced. The cDNA was 207 bp in length and showed 99% homology with that of common sheep registered in GenBank from the starting position (86) to 292 bp. The cDNA sequence encodes 69 amino acid residues and contains a signal peptide sequence which has a GLUCA domain characterizing glucagon-like hormone at amino acid 31 to 57.

参考文献/References:


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备注/Memo

备注/Memo:
收稿日期：2014-11-25 基金项目：四川省学术和技术带头人培养资金重点项目;四川省应用基础项目(2006J13-116);四川省教育厅项目（10ZB142） 作者简介：王金玲(1981-),女,内蒙古牙克石人，硕士,主要研究方向为动物遗传育种。（E-mail）494878248@qq.com 通讯作者：王永(1962-),（E-mail）wangyong010101@swun.cn
更新日期/Last Update: 2015-06-30