[1]徐小波,冯宇,陆志强,等.二花脸猪产仔数性状的分子标记及其效应分析[J].江苏农业学报,2015,(03):579-582.[doi:10.3969/j.issn.1000-4440.2015.03.018]
 XU Xiao-bo,FENG Yu,LU Zhi-qiang,et al.Molecular markers of litter size and its effect in Erhualian sows[J].,2015,(03):579-582.[doi:10.3969/j.issn.1000-4440.2015.03.018]
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二花脸猪产仔数性状的分子标记及其效应分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年03期
页码:
579-582
栏目:
畜牧兽医·水产养殖
出版日期:
2015-06-30

文章信息/Info

Title:
Molecular markers of litter size and its effect in Erhualian sows
作者:
徐小波1冯宇2陆志强2胡东卫2陈哲1
(1.江苏省农业科学院畜牧研究所,江苏南京210014;2.江苏省常熟市畜牧兽医站,江苏常熟215500)
Author(s):
XU Xiao-bo1FENG Yu2LU Zhi-qiang2HU Dong-wei2CHEN Zhe1
(1.Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2.Animal Husbandry and Veterinary Station of Changshu City, Jiangsu Province, Changshu 215500, China)
关键词:
二花脸猪产仔数分子标记
Keywords:
Erhualian sowslitter sizemolecular marker
分类号:
S828.2
DOI:
10.3969/j.issn.1000-4440.2015.03.018
文献标志码:
A
摘要:
采用PCR及PCR-RFLP技术对FSHβ、ESR和PRLR基因在二花脸猪群体内的多态性及对繁殖性状的影响进行了检测与分析。结果表明:108头二花脸猪中检测到FSHβ基因的AA和AB基因型,ESR和PRLR基因的AA、AB和BB 3种基因型。FSHβ基因的AA基因型初产和经产的总产仔数(TNB)和产活仔数(NBA)均显著或极显著高于AB基因型;ESR基因的BB基因型初产及经产TNB和NBA均显著高于AB基因型和AA基因型;PRLR基因的AA基因型和AB基因型初产和经产TNB和NBA均高于BB基因型(P<0.01)。FSHβ基因的AB基因型初产和经产初生质量(BW)和断奶质量(WW)均高于AA基因型(P>0.05);ESR基因的AA基因型初产和经产BW高于AB和BB基因型(P<0.05),WW高于BB基因型;PRLR基因的BB基因型初产和经产仔猪的WW均显著高于AA基因型(P<0.05)。FSHβ、PRLR和ESR基因对二花脸猪繁殖性状有一定影响,可作为二花脸猪繁殖性状辅助选择的标记。
Abstract:
The estrogen receptor (ESR) gene, prolactin receptor (PRLR) gene and follicle-stimulating hormone β submit (FSHβ) gene of Erhualian sows were detected using PCR and PCR-RFLP for the polymorphisms and their effects on reproductive performance. AA and AB genotypes for FSHβ gene, and AA, AB and BB genotypes for ESR and PRLR genes were found in 108 sows. The total number born (TNB) and number born alive (NBA ) of primiparous or multiparous sows with AA genotype for FSHβ gene were higher than those with AB genotype (P<0.05 or P<0.01). The TNB and NBA of primiparous or multiparous sows with BB genotype for ESR gene were higher than those with AA genotype (P<0.01) and AB genotype (P<0.05). The TNB and NBA of primiparous or multiparous sows with AA and AB genotypes for PRLR gene were higher than those with BB genotype (P<0.01). The birth weight (BW) and weaning weight (WW) of primiparous or multiparous sows with AB genotype for FSHβ gene were higher than those with AA genotype (P>0.05). The BW of primiparous or multiparous sows with AA genotype for ESR gene were higher than those with AB and BB genotypes (P< 0.05), and the WW were higher than those with BB genotype (P<0.05). The WW of primiparous or multiparous sows with BB genotype for PRLR gene were higher than those with AA genotype (P<0.05). In conclusion, FSHβ, ESR and PRLR genes contributed to the reproductive traits in Erhualian sows, and could be used as important genetic markers for reproductive traits selection in Erhualian sows.

参考文献/References:


[1]ROTHSCHILD M,JACOBSON C,VASKE D,et al.The estrogen receptor locus is associated with a major gene influencing litter size in pigs [J].Pnas USA, 1996,93(1):201-205.
[2]赵要风,李宁,肖璐,等.猪FSHβ亚基基因结构区逆转座子插入突变及其与猪产仔数关系的研究[J].中国科学(C辑),1999,29 (1):81-86.
[3]VAN RENS B T,EVANS G J,VAN DER LENDE T.Components of litter size in gilts with different prolactin receptor genotypes [J].Theriogenology,2003,59 (3-4):915-926.
[4]周利华,肖石军,杨广成,等.猪表皮生长因子基因的遗传变异及其与产仔性状的相关性分析[J].中国畜牧兽医,2010,37(5):147-150.
[5]SHORT T H,ROTHSCHILD M F,SOUTHWOOD O I,et al. Effect of the estrogen receptor locus on reproduction and production traits in four commercial lines [J]. J Anim Sci,1997,75(12):3138-3142.
[6]DROGEMULLER C,HAMANN H,DISTL O.Candidate gene markers for litter size in different German pig lines [J].J Anim Sci,2001,79(10):2565-2570.
[7]陈克飞,黄路生,李宁,等.猪雌激素受体(ESR)基因对产仔数性状的影响[J].遗传学报,2000,27(10):853-857.
[8]李婧,孟和,李长龙,等.ESR和PRLR基因对民猪产仔数的影响[J].黑龙江畜牧兽医,2003(4):11-12.
[9]柳淑芳,杜立新,闫艳春.莱芜黑猪 FSHβ亚基基因的多态性分析[J].山东农业大学学报,2002, 33 (4):403-408.
[10]〖JP2〗李婧,杨润清,孟和,等.民猪产仔数性状四个候选基因效应分析[J].上海交通大学学报:农业科学版,2004,22(1):74-77.
[11]〖JP2〗李国治,连林生,鲁绍雄,等.撒坝猪促卵泡素β亚基基因多态性及其与初产产仔性能相关性研究[J].养猪,2005(2):22-23.
[12]丁家桐,葛红山,姜勋平,等.猪雌激素受体基因与产仔数和乳头数的关系[J].南京农业大学学报,2002,25 (2):79-81.
[13]VINCENT A I,TUGGLE C K,ROTHSCHILD M F,et al.The prolactin receptor gene is associated with increased litter size in pig [R].Armidale:Proceedings of the 6th World Congress on Genetics Applied to Livestock Production,1998.
[14]VAN RENS B T,VAN DER L T.Litter size and piglet traits of gilts with different prolactin receptor genotypes [J].Theriogenology,2002,57 (2):883-893.
[15]孙延晓,曾勇庆,陈其美,等.八个猪种PRLR和RBP4基因PCR-RFLP检测及群体遗传特性研究[J].山东农业大学学报:自然科学版,2008,39(4):544-548.
[16]孙菊英,吕钢进,王友杉,等.嵊县花猪PRLR基因多态性与繁殖性状的相关分析[J].浙江畜牧兽医,2010(3):1-3.
[17]李千军,王立刚,穆淑琴,等.ESR、FSHβ及OPN基因多态性与母猪繁殖性状的关联分析[J].中国畜牧兽医,2010,37(10):139-143.
[18]胡雪松,王希彪.民猪、长白猪及其杂种母猪催乳素受体(PRLR)基因的NaeⅠ多态性与繁殖性状的相关分析[J].畜牧兽医学报,2006,37(11):1135-1140.
[19]谢保胜.应用约束性最大似然法(REML)估计金华猪繁殖性状和生长性状的遗传参数[D].杭州,浙江大学,2002.〖ZK)〗〖FL)〗

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备注/Memo

备注/Memo:
收稿日期:2014-12-30 基金项目:江苏省农业科技自主创新基金项目[CX(12)2036] 作者简介:徐小波(1964-),男,江苏镇江人,学士,副研究员,主要从事地方猪种质资源保存与利用研究。(Tel) 025-84390341;(E-mail)xiaoboxu@sohu.com
更新日期/Last Update: 2015-06-30