[1]郭保平,牛亚茹,徐明龙,等.AMHR2新型转录本AMHR2-SV1的鉴定及其在大鼠组织中的表达[J].江苏农业学报,2015,(02):394-400.[doi:10.3969/j.issn.1000-4440.2015.02.027]
　GUO Bao-ping,NIU Ya-ru,XU Ming-long,et al.AMHR2-SV1, a novel transcript of AMHR2 and its expression in rat tissues[J].,2015,(02):394-400.[doi:10.3969/j.issn.1000-4440.2015.02.027]
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AMHR2新型转录本AMHR2-SV1的鉴定及其在大鼠组织中的表达()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年02期
页码:
394-400
栏目:
畜牧兽医·水产养殖
出版日期:
2015-04-30

文章信息/Info

Title:
AMHR2-SV1, a novel transcript of AMHR2 and its expression in rat tissues
作者:
郭保平; 牛亚茹; 徐明龙; 徐银学
(南京农业大学动物科学技术学院，江苏南京210095)
Author(s):
GUO Bao-ping; NIU Ya-ru; XU Ming-long; XU Yin-xue
(College of Animal Science and Technology/Nanjing Agricultural University, Nanjing 210095, China)
关键词:
AMHR2; AMHR2-SV1; 新型转录本; 表达图谱
Keywords:
anti-Müllerian hormone receptor 2(AMHR2); AMHR2-SV1; novel transcript; expression profile
分类号:
Q786
DOI:
10.3969/j.issn.1000-4440.2015.02.027
文献标志码:
A
摘要:
本研究旨在鉴定抗苗勒氏管激素受体2（AMHR2）一种新的转录本，确定其在大鼠组织中的表达情况。采用TRIzol法提取组织总RNA；运用RT-PCR和克隆测序技术分析AMHR2基因的可变剪接体及其在大鼠各组织器官中的表达差异，同时利用NCBI ORF Finder识别剪接体序列阅读框，运用ExPASy-ProtParam和Conserved domains软件分别预测剪接体氨基酸构成及其性质和剪接体保守序列。AMHR2-SV1是AMHR2的一种新型转录本，命名为AMHR2-splice variant（AMHR2-SV1），与AMHR2参考序列比较，缺失第10外显子137 bp。利用NCBI ORF Finder 和ExPASy-ProtParam，预测AMHR2-SV1编码429个氨基酸残基，其编码的蛋白质分子量为45 990.7。Conserved domains在线软件分析表明：AMHR2-SV1蛋白序列比完整AMHR2蛋白序列缺少128个氨基酸，丝氨酸/苏氨酸蛋白激酶结构域不完整。检测结果表明，大鼠组织中均存在AMHR2和AMHR2-SV1两个转录本。在肝、脾、肺、肾、肾上腺和子宫组织中AMHR2表达量均高于AMHR2-SV1，差异达到显著水平；两种转录本在不同组织中表达趋势相同，卵巢中表达量最高，心脏中次之，其他组织表达量均显著低于卵巢和心脏。
Abstract:
A splice variant of anti-Müllerian hormone receptor 2 (AMHR2)， AMHR2-splice variant(AMHR2-SV1)， was identified by RT-PCR and sequencing techniques. AMHR2-SV1 contains on open reading frame of 76-1 365 bp, encoding 429 amino acids. Sequence alignment revealed an exon 10(137 bp) deletion in AMHR2-SV1. Protein AMHR2-SV1 has a molecular mass of 45 990.7. Compared to the protein sequence of AMHR2, the protein sequence of AMHR2-SV1 missed 128 amino acids, and lost part of the serine/threonine kinase domain. Both AMHR2 and AMHR2-SV1 transcripts were detected in most rat tissues and organs, and the mRNA for the spliced variant was relatively less abundant than AMHR2

参考文献/References:


[1]DI CLEMENTE N, WILSON C, FAURE E, et al. Cloning, expression, and alternative splicing of the receptor for anti-Mullerian hormone[J]. Mol Endocrinol， 1994, 8(8): 1006-1020.
[2]BAARENDS W M, VAN HELMOND M J, POST M, et al. A novel member of the transmembrane serine/threonine kinase receptor family is specifically expressed in the gonads and in mesenchymal cells adjacent to the mullerian duct[J]. Development， 1994, 120(1): 189-197.
[3]IMBEAUD S, FAURE E, LAMARRE I, et al. Insensitivity to anti-mullerian hormone due to a mutation in the human anti-mullerian hormone receptor[J]. Nat Genet.， 1995, 11(4): 382-388.
[4]BELVILLE C, MARECHAL J D, PENNETIER S, et al. Natural mutations of the anti-Mullerian hormone type II receptor found in persistent Mullerian duct syndrome affect ligand binding, signal transduction and cellular transport[J]. Hum Mol Genet， 2009, 18(16): 3002-3013.
[5]CUTTING A D, AYERS K, DAVIDSON N, et al. Identification, expression, and regulation of anti-Mullerian hormone type-II receptor in the embryonic chicken gonad[J]. Biol Reprod， 2014, 90(5): 106.
[6]IMHOFF F M, YANG D, MATHEW S F, et al. The type 2 anti-Mullerian hormone receptor has splice variants that are dominant-negative inhibitors[J]. FEBS Let， 2013, 587(12): 1749-1753.
[7]边菁. TGF-β信号中 TGF-βRⅡ的突变对膀胱癌侵袭转移的影响及其机制[D]. 武汉：华中科技大学, 2011.
[8]BAKKUM-GAMEZ J N, ALETTI G, LEWIS K A, et al. Mullerian inhibiting substance type II receptor (MISIIR): a novel, tissue-specific target expressed by gynecologic cancers[J]. Gynecol Oncol， 2008, 108(1): 141-148.
[9]MEULMEESTER E, TEN D P. The dynamic roles of TGF-beta in cancer[J]. J Pathol， 2011, 223(2): 205-218.
[10]BRUCE D L, SAPKOTA G P. Phosphatases in SMAD regulation[J]. FEBS Lett， 2012, 586(14): 1897-1905.
[11]郭芳,罗国静,鞠志花,等. SPEF2基因可变剪接体和功能性SNP鉴定及其与公牛精液性状的相关性研究[J]. 南京农业大学学报,2014, 18(3):119-125.
[12]GRAVELEY B R. Alternative splicing: increasing diversity in the proteomic world[J]. Trends Genet， 2001, 17(2): 100-107.
[13]GUO W, DONG Z, GUO Y, et al. Concordant repression and aberrant methylation of transforming growth factor-beta signaling pathway genes occurs early in gastric cardia adenocarcinoma[J]. Mol Biol Rep， 2012, 39(10): 9453-9462.
[14]XIE W, BHARATHY S, KIM D, et al. Frequent alterations of Smad signaling in human head and neck squamous cell carcinomas: a tissue microarray analysis[J]. Oncol Res， 2003, 14(2): 61-73.
[15]MYATT N, ARISTODEMOU P, NEALE M H, et al. Abnormalities of the transforming growth factor-beta pathway in ocular melanoma[J]. J Pathol， 2000, 192(4): 511-518.
[16]JAKOWLEW S B. Transforming growth factor-beta in cancer and metastasis[J]. Cancer Metastasis Rev， 2006, 25(3): 435-457.
[17]LOWERY J W, DE CAESTECKER M P. BMP signaling in vascular development and disease[J]. Cytokine Growth Factor Rev， 2010, 21(4): 287-298.
[18]SHI Y, MASSAGUE J. Mechanisms of TGF-beta signaling from cell membrane to the nucleus[J]. Cell， 2003, 113(6): 685-700.
[19]ANTTONEN M, FARKKILA A, TAURIALA H, et al. Anti-Mullerian hormone inhibits growth of AMH type II receptor-positive human ovarian granulosa cell tumor cells by activating apoptosis[J]. Lab Invest， 2011, 91(11): 1605-1614.
[20]蒋香菊,吴阳升,林嘉鹏,等. 绵羊AMH基因的克隆, 序列分析与原核表达[J].江苏农业学报, 2013, 29(6): 1386-1392.
[21]KEDEM A, HOURVITZ A, YUNG Y, et al. Anti-Mullerian hormone (AMH) downregulation in late antral stages is impaired in PCOS patients. A study in normo-ovulatory and PCOS patients undergoing in vitro maturation (IVM) treatments[J]. Gynecol Endocrinol，2013, 29(7): 651-656.
[22]KOTANIDIS L, ASIMAKOPOULOS B, NIKOLETTOS N. Association between AMH, oocyte number and availability of embryos for cryopreservation in IVF[J]. In Vivo， 2013, 27(6): 877-880.
[23]PEIGNE M, DECANTER C. Serum AMH level as a marker of acute and long-term effects of chemotherapy on the ovarian follicular content: a systematic review[J]. Reprod Biol Endocrinol， 2014, 12: 26.
[24]JOSSO N, DI CLEMENTE N. Serine/threonine kinase receptors and ligands[J]. Curr Opin Genet Dev， 1997, 7(3): 371-377.
[25]KAMIYA T, KAI W, TASUMI S, et al. A trans-species missense SNP in Amhr2 is associated with sex determination in the tiger pufferfish, Takifugu rubripes (fugu)[J]. PLoS Genet， 2012, 8(7): e1002798.
[26]GEORGOPOULOS N A, KARAGIANNIDOU E, KOIKA V, et al. Increased frequency of the anti-mullerian-inhibiting hormone receptor 2 (AMHR2) 482 A>G polymorphism in women with polycystic ovary syndrome: relationship to luteinizing hormone levels[J]. J Clin Endocrinol Metab， 2013, 98(11): E1866-E1870.

备注/Memo

备注/Memo:
收稿日期：2014-08-27 基金项目：南京农业大学科技发展基金项目（0606F0025） 作者简介：郭保平（1988-），男，山西太原人，硕士研究生，研究方向为动物遗传育种与繁殖。（Tel）18761867090；(E-mail) 2012105038@njau.edu.cn 通讯作者：徐银学，(E-mail) xuyinxue@njau.edu.cn
更新日期/Last Update: 2015-04-30