[1]黄欣梅,李银,刘宇卓,等.华东地区H9N2亚型禽流感病毒HA基因的遗传演化分析[J].江苏农业学报,2015,(02):382-388.[doi:10.3969/j.issn.1000-4440.2015.02.025]
　HUANG Xin-mei,LI Yin,LIU Yu-zhuo,et al.Phylogenetic analysis of hemagglutinin genes of H9N2 subtype avian influenza viruse in Eastern China[J].,2015,(02):382-388.[doi:10.3969/j.issn.1000-4440.2015.02.025]
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华东地区H9N2亚型禽流感病毒HA基因的遗传演化分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年02期
页码:
382-388
栏目:
畜牧兽医·水产养殖
出版日期:
2015-04-30

文章信息/Info

Title:
Phylogenetic analysis of hemagglutinin genes of H9N2 subtype avian influenza viruse in Eastern China
作者:
黄欣梅; 李银; 刘宇卓; 赵冬敏; 杨婧; 韩凯凯
（江苏省农业科学院兽医研究所，农业部兽用生物制品工程技术重点实验室，国家兽用生物制品工程技术研究中心，江苏南京210014）
Author(s):
HUANG Xin-mei; LI Yin; LIU Yu-zhuo; ZHAO Dong-min; YANG Jing; HAN Kai-kai
（Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture/National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China）
关键词:
H9N2亚型禽流感病毒; HA基因; 进化分析
Keywords:
H9N2 subtype avian influenza virus; HA gene; phylogenetic analysis
分类号:
S852.65
DOI:
10.3969/j.issn.1000-4440.2015.02.025
文献标志码:
A
摘要:
为了解华东地区H9N2亚型禽流感病毒（AIV）的遗传特点和流行规律，利用RT-PCR方法扩增华东地区2008~2013年分离的17株H9N2亚型AIV血凝素（HA）基因片段，进行序列测定和遗传进化分析，并对HA蛋白质的裂解位点、受体结合位点和潜在的糖基化位点进行分析。结果显示，17株H9N2亚型AIV HA基因核苷酸和推导的氨基酸同源性分别为84.6%~99.3%和86.0%~99.5%，均属于欧亚分支。其中15株鸡源病毒属于Y280-like亚系，2株鸭源病毒属于G1-like亚系。HA裂解位点都是非连续碱性氨基酸，属于低致病力毒株。HA受体结合位点149、150、191、198和234位氨基酸存在变异，尤其是234位氨基酸有14株毒株变为L，表现出人流感病毒受体结合特性。有2株毒株在145位氨基酸出现新的糖基化位点，5个毒株在218位氨基酸缺失1个糖基化位点，13个毒株在313位氨基酸增加了一个新的糖基化位点。研究结果表明华东地区近年来H9N2亚型AIV存在一定的变异，应加强对该类病毒的分子流行病学监测。
Abstract:
To study the genetic characteristics and epidemiology of H9N2 subtype avian influenza virus (AIV) isolated from avian in Eastern China, the hemagglutinin (HA) gene of 17 H9N2 AIVs isolated from 2008 to 2013 were amplified by RT-PCR. Sequencing and Phylogenetic analysis were performed, and the molecular characteristics of the motif of HA protein cleavage site, the receptor binding sites and the potential glycosylation sites were analyzed. The HA genes of these strains shared nucleotide and deduced amino acid homologies ranging from 84.6% to 99.3% and 86.0% to 99.5 %, respectively. All the 17 strains belonged to Eurasian lineage, among which, 15 chicken-derived isolates belonged to Y280-like sublineage, and 2 duck-derived isolates belonged to G1-like sublineage. The cleavage site motif of all isolates lacked multiple basic amino acids, which made them low pathogenic strains. The amino acids variants resided at the sites 149, 150, 191, 198 and 234 of the receptor binding sites. 14 out of the 17 isolates had a leucine residue at site 234, indicative of the characteristic of human influenza virus-like receptor. Due to gene mutation, an additional potential glycosylation site were found at amino acid site 145 of 2 isolates and site 313 of 13 isolates, and a glycosylation site deletion took place at amino acid site 218 of 5 isolates. The continuing evolution of H9N2 subtype AIVs in Eastern China emphasizes the importance of H9N2 influenza virus surveillance throughout this region.

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备注/Memo

备注/Memo:
收稿日期：2014-08-03 基金项目：江苏省农业科技自主创新基金项目[CX（14）2091] 作者简介：黄欣梅(1981-), 女，福建南安人，硕士，助理研究员，主要从事家禽疫病流行病学和致病分子机制研究。（E-mail）hxmrene@126.com 通讯作者：李银，（E-mail）muziyin08@163.com
更新日期/Last Update: 2015-04-30