[1]杨家大,彭舒.贵州地方山羊品种肌肉生长抑制素基因的表达差异[J].江苏农业学报,2015,(02):369-375.[doi:10.3969/j.issn.1000-4440.2015.02.023]
　YANG Jia-da,PENG Shu.Differential expression of myostatin gene in Guizhou landrace goat[J].,2015,(02):369-375.[doi:10.3969/j.issn.1000-4440.2015.02.023]
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贵州地方山羊品种肌肉生长抑制素基因的表达差异()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年02期
页码:
369-375
栏目:
畜牧兽医·水产养殖
出版日期:
2015-04-30

文章信息/Info

Title:
Differential expression of myostatin gene in Guizhou landrace goat
作者:
杨家大; 彭舒
（凯里学院环境与生命科学学院，贵州凯里556011）
Author(s):
YANG Jia-da; PENG Shu
(College of Environmental and Life Sciences，Kaili University，Kaili 556011，China)
关键词:
肌肉生长抑制素; 实时荧光定量; 贵州地方山羊; 基因表达
Keywords:
myostatin; real-time fluorescence quantitation; Guizhou landrace goat; gene expression
分类号:
S827
DOI:
10.3969/j.issn.1000-4440.2015.02.023
文献标志码:
A
摘要:
为了解贵州地方山羊肌肉生长抑制素基因（Myostatin）的表达部位及组织分布模式，比较各组织中Myostatin基因表达的品种间差异，以β-actin作为内参基因，应用TaqMan实时荧光定量方法检测了黔东南小香羊、贵州白山羊、贵州黑山羊、黔北麻羊等贵州地方山羊品种以及南江黄羊的肝、肾、心、肺、背最长肌、半膜肌、皮下脂肪组织中Myostatin基因mRNA的表达水平。结果显示：肝和皮下脂肪中Myostatin基因的表达水平较高，肾和肺次之，半膜肌、背最长肌和心等肌肉组织中的表达水平相对较低；而半膜肌和背最长肌中Myostatin基因的表达水平高于心肌。肝和皮下脂肪Myostatin基因的表达在各品种间差异不显著；心、肺、肾、背最长肌和半膜肌中Myostatin基因的表达，贵州白山羊显著高于黔东南小香羊和黔北麻羊；肺、背最长肌和半膜肌中，Myostatin基因的表达贵州白山羊显著高于贵州黑山羊和南江黄羊。可见，贵州地方山羊心、肝、肺、肾、背最长肌、半膜肌和皮下脂肪中均有Myostatin基因表达，其组织表达水平总体为肝和皮下脂肪＞肾和肺＞半膜肌、背最长肌和心（肌），且多数组织中Myostatin基因的表达存在品种差异。
Abstract:
In order to figure out the locations and tissue expression patterns of myostatin gene in Guizhou landrace goats, and the varietal expression difference, the expression levels of myostatin mRNA in the liver，kidney，heart，lung，longissimus muscle，semimembranosus muscle and subcutaneous fat of Qiandongnan small Xiang goats, Guizhou white goats, Guizhou black goats, Qianbei Ma goats and Nanjiang yellow goats, were detected by real time fluorescence quantitative TaqMan method and normalized by internal β-actin. The liver and subcutaneous fat exhibited the the highest level of myostatin mRNA expression, follwed by kidney and lung, semimembranosus muscle, longissimus muscle and heart (myocardium) were the lowest. The expression levels in semimembranosus muscle and longissimus muscle were higher than that in liver (myocardium). The myostatin mRNA level in livers and subcutaneous fat showed no difference among breeds. The gene expression level in the hearts, lungs, kidneys, longissimus muscle and semimembranosus muscle of Guizhou white goat were significantly higher than those in Qiandongnan small Xiang goat and Qianbei Ma goat. Guizhou white goat also presented higher levels of myostatin mRNA in the lungs, longissimus muscle and semimembranosus muscle than Guizhou black goat and Nanjiang yellow goat. The results suggested that myostatin gene were expressed in all the tested tissues and organs of Guizhou landrace goats, the level being listed in the order of subcutaneous fat＞kidney and lung＞semimembranosus muscle, longissimus muscle and heart (myocardium). For most tissues, there were differential expressions among breeds.

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备注/Memo

备注/Memo:
收稿日期：2015-02-05 基金项目：凯里学院规划课题（Z1304）；凯里学院获得博士学位（引进教授）教师专项（BS201303）；贵州省科技联合基金项目[黔科合J字LKK(2013)14号]；贵州省普通本科高校自然科学研究项目[黔教合KY字（2014）236]；国家自然科学基金项目（81460572） 作者简介：杨家大（1975-），男，贵州天柱人，博士，副教授，主要从事动物分子遗传学研究。（Tel）0855-8511625；（E-mail）yangjiada2@163.com〖ＨＴ〗〖ＦＱ）〗
更新日期/Last Update: 2015-04-30