[1]张荣,奚永兰,叶小梅,等.棘孢木霉1285对麦秸的降解及厌氧发酵的影响[J].江苏农业学报,2015,(02):328-333.[doi:10.3969/j.issn.1000-4440.2015.02.017]
　ZHANG Rong,XI Yong-lan,YE Xiao-mei,et al.Degradation and anaerobic fermentation of wheat straw affected by Trichoderma asperellum 1285[J].,2015,(02):328-333.[doi:10.3969/j.issn.1000-4440.2015.02.017]
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棘孢木霉1285对麦秸的降解及厌氧发酵的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年02期
页码:
328-333
栏目:
耕作栽培·资源环境
出版日期:
2015-04-30

文章信息/Info

Title:
Degradation and anaerobic fermentation of wheat straw affected by Trichoderma asperellum 1285
作者:
张荣12; 奚永兰2; 叶小梅12; 徐蓉2; 常志州2; 李加友3; 洪青1; 徐跃定2
（1.南京农业大学生命科学学院，江苏南京210095；2.江苏省农业科学院农业资源与环境研究所，江苏南京210014；3.嘉兴学院生物与化学工程学院，浙江嘉兴314001）
Author(s):
ZHANG Rong12; XI Yong-lan2; YE Xiao-mei12; XU Rong2; CHANG Zhi-zhou2; LI Jia-you3; HONG Qing1; XU Yue-ding2〖JP〗
(1.College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China；2.Institute of Agricultural Resource and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China；3.College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing 314001, China)
关键词:
棘孢木霉; 生物预处理; 厌氧发酵; 甲烷
Keywords:
Trichoderma asperellum; biological pretreatment; anaerobic fermentation; methane
分类号:
X172
DOI:
10.3969/j.issn.1000-4440.2015.02.017
文献标志码:
A
摘要:
为了提高秸秆厌氧发酵产气效率，本研究以1株新分离的秸秆降解菌棘孢木霉（Trichoderma asperellum 1285）对麦秸木质纤维进行生物预处理，研究T. asperellum 1285对麦秸木质纤维的降解效果，并确定最佳的生物预处理时间；随后将生物预处理的秸秆原料用于中温 [（37±1） ℃]厌氧发酵产沼气研究。结果显示：T. asperellum 1285对麦秸的木质纤维具有较好的降解能力，可有效降解木质素，保留纤维素并降低干物质损失率,降解预处理8 d时，木质素损失率比对照组提高了53.09 %，而干物质损失率、半纤维素损失率、纤维素损失率分别比对照组降低了55.89 %、16.08 %、50.91 %；扫描电镜、红外光谱结果进一步证明麦秸经T. asperellum 1285预处理，木质素可被有效降解而纤维素保留并暴露在外。将经过T. asperellum1285生物预处理8 d后的麦秸用于中温厌氧消化产沼气，总产气量和总产甲烷量分别为(14 774.30±216.56 ) ml、(7 638.90±165.36) ml，较未经过生物预处理的对照组分别提高14.05 %、16.01 %。表明麦秸经T. asperellum 1285预处理可有效提高麦秸厌氧发酵产气效率。
Abstract:
To improve the biogas production efficiency of straw in anaerobic fermentation, wheat straw was pretreated with Trichoderma asperellum 1285， a newly isolated strain degiading wheat straw. The time of pretreatment for wheat straw by the strain was determined, and the effect of pretreated wheat straw on anaerobic fermentation at mesospheric temperature [（37±1） ℃] was investigated. T. asperellum 1285 showed strong degradation abilities for the lignocelluloses of wheat straw，especially for lignin. Compared with control, the degradation rate of lignin was improved by 53.09%， while the degradation rates of dry matter, hemicelluloses and cellulose were reduced by 55.89%, 16.08% and 50.91%, respectively 8 d after degradation. Scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR) confirmed the effective degradation of lignin and intactly maintained cellulose. Total gas production and methane production of pretreated wheat straw for eight day in anaerobic fermentation were (14 774.30±216.56) ml and (7 638.90±165.36) ml, 14.05 % and 16.01 % higher than those of control. The results indicated that T. asperellum 1285 could improve the anaerobic fermentation efficiency of wheat straw.

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备注/Memo

备注/Memo:
收稿日期：2014-11-12 基金项目：江苏省农业科技自主创新基金项目[CX(12)1002] 作者简介：张荣（1989-），女，山东临沂人，硕士，主要从事农业固废物资源化利用研究。(Tel)18761869663；(E-mail)0309linlin@163.com 通讯作者：叶小梅， (E-mail)yexiaomei610@126.com
更新日期/Last Update: 2015-04-30