[1]赵慧,徐迎春,张翔,等.外源NO对铜、镉胁迫下狭叶香蒲生理反应的影响[J].江苏农业学报,2015,(02):260-266.[doi:10.3969/j.issn.1000-4440.2015.02.006]
　ZHAO Hui,XU Ying-chun,ZHANG Xiang,et al.Physiological response of Typha angustifolia to exogenous nitric oxide under copper and cadmium stress[J].,2015,(02):260-266.[doi:10.3969/j.issn.1000-4440.2015.02.006]
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外源NO对铜、镉胁迫下狭叶香蒲生理反应的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年02期
页码:
260-266
栏目:
遗传育种·生理生化
出版日期:
2015-04-30

文章信息/Info

Title:
Physiological response of Typha angustifolia to exogenous nitric oxide under copper and cadmium stress
作者:
赵慧; 徐迎春; 张翔; 董春兰
（南京农业大学园艺学院，江苏南京210095）
Author(s):
ZHAO Hui; XU Ying-chun; ZHANG Xiang; DONG Chun-lan
(College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China)
关键词:
狭叶香蒲; 硝普钠; 抗氧化酶; 丙二醛 
Keywords:
Typha angustifolia; sodium nitroprusside; antioxidase; malondialdehyde
分类号:
S68
DOI:
10.3969/j.issn.1000-4440.2015.02.006
文献标志码:
A
摘要:
为研究外源一氧化氮(NO)对狭叶香蒲耐Cu、Cd胁迫的影响，进而揭示其缓解Cu、Cd胁迫的生理机制，以狭叶香蒲幼苗为试材，在Cu或Cd的胁迫下，分别添加0 μmol/L、50 μmol/L、75 μmol/L和100 μmol/L SNP（硝普钠，为NO供体），检测叶片和根系中抗氧化酶活性以及丙二醛（MDA）、抗坏血酸、蛋白质和叶绿素含量。结果表明：在25 mg/L Cu2+或1.5 mg/L Cd2+处理下施加不同浓度的SNP引起反应的生理指标有所不同，总体表现为叶片和根中超氧化物歧化酶（SOD）、过氧化物酶（POD）活性和蛋白质、抗坏血酸含量升高，MDA含量下降，叶绿素含量不受影响。说明外源NO能在一定程度上缓解Cu及Cd胁迫对狭叶香蒲幼苗造成的伤害。
Abstract:
Studies on the effects of exogenous nitric oxide(NO) on the physiological reaction of Typha angustifolia in response to copper and cadmium stress will uncover the underlying mechanism of stress relief. In this study, the physiological responses of the leaves and roots of T. angustifolia seedlings applied with 0 μmol/L, 50 μmol/L, 75 μmol/L and 100 μmol/L SNP (sodium nitroprusside, NO donor) under Cu or Cd stress were detected. Different concentrations of SNP led to various physiological responses of leaves and roots subjected to the stress of 25 mg/L Cu2+ or 1.5 mg/L Cd2+. The overall performance was that the activities of superoxide dismutase(SOD), peroxidase (POD) and the contents of proteins and ascorbic acid were increased in the leaves and roots of T. angustifolia, while MDA content was decreased. The chlorophyll content had no significant change. These results indicated that the damage caused by Cu and Cd stress was alleviated by SNP to a certain degree.〖JP〗

参考文献/References:


[1]陈桂珠,李柳川. 香蒲植物净化塘复合系统的结构和功能[J].有色金属环保, 1990（4）: 91-97.
[2]吴晓丽,罗玉明,徐迎春,等. 镉胁迫对狭叶香蒲某些生理指标的影响[J].植物资源与环境学报, 2007, 16(1): 74-76.
[3]赵艳,徐迎春,柴翠翠,等. 铜胁迫对狭叶香蒲生长及生理特性的影响[J].广西植物, 2010, 30(3): 367-372.
[4]赵艳,徐迎春,吴晓丽,等. Cu胁迫对狭叶香蒲体内元素吸收分配的影响[J].生态学杂志, 2009, 28(4): 665-670.
[5]连建军,许士国,韩成伟. 芦苇和香蒲对重金属钼的吸收特性研究[J]. 环境科学, 2011, 32(11): 3335-3340.
[6]DURNER J, KLESSIG D F. Nitric oxide as a signal in plants[J]. Current Opinion in Plant Biology, 1999, 2: 369-374.
[7]WENDEHENNE D, DURNER J, KLESSIG D F. Nitric oxide: A new player in plant signaling and defence responses[J]. Current Opinion in Plant Biology, 2004, 7: 449-455.
[8]HU K D, HU L Y, LI Y H, et al. Protective roles of nitric oxide on germination and antioxidant metabolism in wheat seeds under copper stress[J]. Journal of Plant Growth Regulation, 2007, 53: 173-183.
[9]李海燕,郭永成,李刘洋,等. 外源一氧化氮对镉胁迫下玉米幼苗根生长及氧化伤害的影响[J]. 西北植物学报, 2012, 32(8): 1599-1605.
[10]LASPINA N V, GROPPA M D, TOMARO M L, et al. Nitric oxide protects sunflower leaves against Cd-induced oxidative stress[J]. Plant Science, 2005, 169: 323-330.
[11]ZHANG Z Y, WANG H H, WANG X M, et al. Nitric oxide enhances aluminum tolerance by affecting cell wall polysaccharides in rice roots[J]. Plant Cell Reports, 2011, 30: 1701-1711. 
[12]段凯旋,杨洪刚,冉坤,等. 一氧化氮对铜、镉胁迫下平邑甜茶幼苗活性氧代谢的影响[J]. 中国农学通报, 2007, 23(10): 104-109.
[13]李德文,李美兰,于景华,等. 外源NO对UV-B胁迫下红豆杉抗氧化系统的影响[J]. 生态学杂志, 2012, 31(9): 2203-2208.
[14]KUMAR G N M, KNOWLES N R. Change in lipid peroxidation and lipolytic and free-radical scavenging enzyme activities during aging and sprouting of potato (Solanum tuberosum)seed-tubers[J]. Plant Physiology, 1993, 102: 115-124.
[15]李和生. 植物生理生化实验原理和技术[M].北京:高等教育出版社, 2000:134-137.
[16]陈建勋.植物生理实验指导[M].广州：华南理工大学出版社, 2002:69-70.
[17]ZHOU W, ZHAO D, LIN X. Effects of water logging on nitrogen accumulation and alleviation of waterlogging damage by application of nitrogen fertilizer and mixtalol in winter rape(Brassica napes L.)[J]. Journal of Plant Growth Regulation, 1997, 16: 47-53.
[18]QUINTANILLA-GUERREO F, DUARTE-VAZQUEZ M A, GARCIA-ALMENDAREZ B E, et al. Polyethylene glycol improves phenol removal by immobilized turnip peroxidase[J]. Bioresource Technology, 2008, 99(18): 8605-8611.
[19]黄文敏,邢伟,李敦海,等. 外源抗坏血酸对烟草细胞生长及衰老的影响[J]. 农业环境科学学报, 2006, 25(5): 1157-1161.
[20]张力思，王洁，艾呈祥，等，外源NO对干旱胁迫下君迁子幼苗光合作用的影响[J]．江苏农业学报，2014，30(3)：623-628.
[21]方淑梅，梁喜龙，纪伟波，等．外源NO对盐碱胁迫下水稻幼苗生长抑制的缓解作用[J]．江苏农业科学，2013，41(8)：67-69.
[22]尹丹丹，薛泽云，徐迎春，等．外源一氧化氮对黄长筒石蒜子球发育的影响[J]．江苏农业科学，2013，41(9)：153-156.
[23]MATA C G，LAMATTINA L. Nitric oxide induces stomatal closure and enhances the adaptive plant responses against drought stress[J]．Plant Physiology, 2001, 126: 1196-1204．
[24]吴业飞,吴鲁阳,张振文. 紫外线-B 辐射增强对葡萄叶片抗氧化系统的影响[J]. 西北农林科技大学学报:自然科学版, 2008, 36(12): 161-166. 
[25]徐严,魏小红,李兵兵. 外源NO对重金属Cd胁迫下亚麻幼苗叶片抗氧化能力的影响[J]. 甘肃农业大学学报, 2012, 47(5): 45-49.
[26]赵宝泉,万宇,杨世湖,等. 外源NO供体硝普钠( SNP)对重金属Cd胁迫下水稻幼苗膜脂过氧化及抗氧化酶的影响[J]. 江苏农业学报, 2010, 26(3): 468-475.
[27]曹仪植,宋占午. 植物生理学[M]. 兰州: 兰州大学出版社, 1998:102-105.
[28]樊怀福,郭世荣,焦彦生,等. 外源一氧化氮对NaCl胁迫下黄瓜幼苗生长、活性氧代谢和光合特性的影响[J]. 生态学杂志, 2007, 27(2): 546-553.
[29]AUGUST C F, SHIZUE M, BIRGIT O．Photoinhibition carotenoid composition and the co-regulation of photochemical and nonphotochemical quenching in neotropical savanna trees[J]．Tree Physiology , 2007, 7(5):717-725．
[30]MCNULTY H P，BYUN J，LOCKWOOD S F，et al． Differential effects of carotenoids on lipid peroxidation due to membrane interactions: X-ray diffraction analysis[J]．Biochimica et Biophysica Acta-biomembranes, 2007,1768(1) : 167-174．〖ZK）〗〖FL）〗

备注/Memo

备注/Memo:
收稿日期：2014-07-09 基金项目：江苏省自然科学基金项目（BK2011640）  作者简介：赵慧（1988-），女，江苏淮安人，硕士研究生，研究方向为观赏植物生理生态。（E-mail）zhaohui5951@126.com 通讯作者：徐迎春, （E-mail）xyc@njau.edu.cn
更新日期/Last Update: 2015-04-30