[1]卢松茂,陈振东,林秀香,等.基于 rDNA-ITS 序列的天门冬拟茎点霉与相似种的系统发育关系[J].江苏农业学报,2015,(01):62-67.[doi:10.3969/j.issn.1000-4440.2015.01.009]
　LU Song-mao,CHEN Zhen-dong,LIN Xiu-xiang,et al.Phylogenetic relationships of Phomopsis asparagi and related speices based on internal transcribed space of ribosomal DNA(rDAN-ITS) sequences[J].,2015,(01):62-67.[doi:10.3969/j.issn.1000-4440.2015.01.009]
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基于 rDNA-ITS 序列的天门冬拟茎点霉与相似种的系统发育关系()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年01期
页码:
62-67
栏目:
植物保护
出版日期:
2015-02-28

文章信息/Info

Title:
Phylogenetic relationships of Phomopsis asparagi and related speices based on internal transcribed space of ribosomal DNA(rDAN-ITS) sequences
作者:
卢松茂; 陈振东; 林秀香; 郑少缘; 余智城
（福建省热带作物科学研究所，福建漳州363001）
Author(s):
LU Song-mao; CHEN Zhen-dong; LIN Xiu-xiang; ZHENG Shao-yuan; YU Zhi-cheng
（Fujian Institute of Tropical Crops, Zhangzhou 363001, China）
关键词:
天门冬拟茎点霉; 拟茎点霉属; 核糖体DNA内转录间隔区; 分子系统学
Keywords:
Phomopsis asparagi; Phomopsis; internal transcribed space (ITS) of ribosomal DNA; molecular phylogenetics
分类号:
S644.6
DOI:
10.3969/j.issn.1000-4440.2015.01.009
文献标志码:
A
摘要:
芦笋茎枯病由天门冬拟茎点霉（Phomopsis asparagi）引起，为明确P. asparagi与寄生在其他蔬菜、水果和经济林木等植物上的拟茎点霉不同种之间的系统发育关系，对分离至福建漳州的P. asparagi菌株及从GenBank下载的相关菌株的35条ITS序列进行多序列比对分析，并用Neighbor-Joining法构建系统发育树。系统发育分析结果显示：35个菌株被划分为Ⅰ、Ⅱ和Ⅲ组群；P. asparagi与葡萄拟茎点霉（P. viticola）、叶下朱生拟茎点霉（P. phyllanthicola）、杨桃拟茎点霉（P. averrhoae）、大豆茎溃疡病菌（Diaporthe phaseolorum）聚在I组群的A亚群，它们的系统学关系最近；大豆拟茎点霉(P. longicolla)、大豆间座壳菌(Diaporthe sojae)、褐纹拟茎点霉(P. vexans)、光叶子花拟茎点霉(P. glabrae)、柑橘间座壳菌(Diaporthe citri)、黄瓜间座壳菌(Diaporthe sclerotioides)聚在Ⅱ组群，它们与P. asparagi的系统学关系较远；昏暗拟茎点霉(P. obscurans)单独聚在最远的分支上（Ⅲ组群），其与P. asparagi的系统学关系最远。
Abstract:
To define the phylogenetic relationships of Phomopsis asparagi and related speices that parasitized other vegetables, fruits and economic trees, 35 internal transcribed space (ITS) of ribosomal DNA (rDNA-ITS) sequences of P. asparagi isolated from Zhangzhou city of Fujian province and the related speices downloaded from GenBank were together processed by multiple sequences alignment analysis, and the phylogenetic tree was constructed by Neighbor-Joining method. The Phomopsis strains were clustered into group I, group II and group III. P. asparagi, P. viticola, P. phyllanthicola, P. averrhoae and Diaporthe phaseolorum were clustered together in subgroup A of group I. They were closely related, but were relatively differentiated to some extent. P. longicolla, Diaporthe sojae, P. vexans, P. glabrae, Diaporthe citri and Diaporthe sclerotioides were clustered in group II. They had a distant relationship with P.asparagi. P. obscurans was clustered in the farthest distance clade(group III), indicative of the farthest phylogenetic relationship with P.asparagi.

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备注/Memo

备注/Memo:
收稿日期：2014-07-28 基金项目：福建省公益类科研院所专项（2010R1030-1） 作者简介：卢松茂（1983-），男，福建永定人，本科，助理研究员，主要从事植物病理学研究。（Tel）13860897573；（E-mail） songmaolu@163.com 通讯作者：林秀香，（Tel）0596-6101151
更新日期/Last Update: 2015-02-28