[1]钱瑜,察倩倩,孔敏,等.植物 NRT2 家族的分子进化[J].江苏农业学报,2015,(01):45-54.[doi:10.3969/j.issn.1000-4440.2015.01.007]
　QIAN Yu,CHA Qian-qian,KONG Min,et al.Molecular evolution of NRT2 gene family in plant[J].,2015,(01):45-54.[doi:10.3969/j.issn.1000-4440.2015.01.007]
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植物 NRT2 家族的分子进化()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年01期
页码:
45-54
栏目:
遗传育种·耕作栽培·生理生化
出版日期:
2015-02-28

文章信息/Info

Title:
Molecular evolution of NRT2 gene family in plant
作者:
钱瑜1; 察倩倩2; 孔敏1; 刘照坤3; 李英1; 侯喜林1; 刘同坤1
（1.南京农业大学园艺学院/作物遗传与种质创新国家重点实验室,江苏南京210095；2.南京农业大学食品科技学院,江苏南京210095；3.苏州市蔬菜研究所，江苏苏州215008）
Author(s):
QIAN Yu1; CHA Qian-qian2; KONG Min1; LIU Zhao-kun3; LI Ying1; HOU Xi-lin1; LIU Tong-kun1
（1.College of Horticulture/State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China;2.College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;3.Suzhou Institute of Vegetable Science, Suzhou 215008, China）
关键词:
NRT2; 共线性; 序列分析; 进化
Keywords:
NRT2; synteny; sequence analysis; evolution
分类号:
S601
DOI:
10.3969/j.issn.1000-4440.2015.01.007
文献标志码:
A
摘要:
为了研究硝酸盐转运子NRT2家族在植物中的进化关系，综合利用共线性信息和序列相似性信息，从8种已经进行过全基因组测序的物种（大白菜、大豆、杨树、葡萄、玉米、水稻、高粱、二穗短柄草）中，搜索拟南芥NRT2的同源基因。通过分析发现，这些基因序列基本都具有MFS超家族的NNP家族基因的典型特征。但单、双子叶植物之间，NRT2的基因结构存在着较大的差异。系统发育重建结果表明,NRT2基因家族的成员主要是在单、双子叶植物分歧后进化产生的；同时，不同植物的NRT2基因又具有不同的进化模式。大白菜NRT2家族经历了基因组三倍化事件及基因片段丢失。因此，拟南芥和大白菜的NRT2家族有较为密切的进化关系，进化分析为利用已有的拟南芥NRT2研究结果进一步在大白菜中开展NRT2的功能研究提供了参考线索。
Abstract:
In order to study the evolutionary relationship of NRT2 family of nitrate transporters in plants, synteny analysis approach and sequence similarity were used to identify putative orthologues of Arabidopsis NRT2 genes in other eight fully sequenced genomes (Chinese cabbage, soybean, western poplar, grape vine, maize, rice, sorghum, and purple false brome). Sequence analysis reveals that these sequences belong to the nitrate/nitrite poter (NNP) family of the major facilitator superfamily (MFS). However, there is significant difference in the NRT2 gene structure between monocots and dicots. Phylogentic tree analysis showed that, most members of the NRT2 family developed primarily following the divergence of the monocots and dicots. Simultaneously, the evolution models of NRT2 in different kinds of plants varied. NRT2 family of Chinese cabbage (Brassica rapa) generated the genome triplication and gene loss. Therefore, the NRT2 family in Chinese cabbage has a relatively close evolutionary relationship with that of Arabidopsis.

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备注/Memo

备注/Memo:
收稿日期：2014-06-27 基金项目：国家自然科学基金项目（31301782）；江苏省自然科学基金项目（BK20130673）；中国博士后基金项目（2014M550294）；南京农业大学青年科技创新基金项目（KJ2013015） 作者简介：钱瑜(1989-)，女，江苏苏州人，硕士研究生，主要从事不结球白菜相关研究。（E-mail）pei_1224@126.com 通讯作者：刘同坤，（Tel）025-84395332；（E-mail）liutk@njau.edu.cn
更新日期/Last Update: 2015-02-28