[1]倪先林,赵甘霖,刘天朋,等.SSR 分子标记在糯高粱种质资源遗传多样性分析中的应用[J].江苏农业学报,2015,(01):16-22.[doi:10.3969/j.issn.1000-4440.2015.01.003]
　NI Xian-lin,ZHAO Gan-lin,LIU Tian-peng,et al.Genetic diversity analysis of glutinous sorghum germplasm by simple sequence repeat[J].,2015,(01):16-22.[doi:10.3969/j.issn.1000-4440.2015.01.003]
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SSR 分子标记在糯高粱种质资源遗传多样性分析中的应用()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年01期
页码:
16-22
栏目:
遗传育种·耕作栽培·生理生化
出版日期:
2015-02-28

文章信息/Info

Title:
Genetic diversity analysis of glutinous sorghum germplasm by simple sequence repeat
作者:
倪先林; 赵甘霖; 刘天朋; 胡炯凌; 李元; 陈国民; 汪小楷; 丁国祥
（四川省农业科学院水稻高粱研究所,四川泸州646000）
Author(s):
NI Xian-lin; ZHAO Gan-lin; LIU Tian-peng; HU Jiong-ling; LI Yuan; CHEN Guo-min; WANG Xiao-kai; DING Guo-xiang
（Rice and Sorghum Research Institute, Sichuan Academy of Agricultural Sciences, Luzhou 646000, China）
关键词:
SSR标记; 糯高粱; 遗传多样性
Keywords:
simple sequence repeat(SSR); glutinous sorghum; genetic diversity
分类号:
S514.02
DOI:
10.3969/j.issn.1000-4440.2015.01.003
文献标志码:
A
摘要:
为了阐明糯高粱种质资源之间的亲缘关系，用25对SSR引物评价了29份糯高粱种质资源。结果显示，25对引物共检测出59个等位基因，每对引物检测到2.28个等位基因，平均有效等位基因为1.81个，引物位点的多态信息量变幅为0.17～0.62，平均为0.34。29份糯高粱种质资源的遗传相似系数范围为 0.20～0.95，平均为0.60，UPGMA方法将29份糯高粱品种在遗传相似系数0.48处聚为2大类，其中不同地理来源的品种被聚在同一亚类，农艺性状近似的品种被聚在同一亚类。表明糯高粱品种的亲缘关系与地理来源关系不大，其种质资源具有较为丰富的遗传多样性。
Abstract:
To evaluate the genetic relationship of 29 glutinous sorghum germplasm, 25 pair of simple sequence repeat(SSR) markers were used. A total of 59 alleles were identified with an average of 2.28 alleles per locus and the average effective number of alleles (NE) of 1.81. The average polymorphism information content(PIC) varied widely from 0.17 to 0.62, with an average value of 0.34.The similarity coefficients of 29 glutinous sorghum accessions ranged from 0.20 to 0.95, with an average value of 0.60. Cluster analysis with UPGMA showed that the varieties could be divided into two groups at the genetic similarity coefficient of 0.48. The varieties from different geographic origins were clustered into one subclass, and the varieties with similar agronomic traits were classified into one subclass as well, indicating that the geographical origin were irresponsible for the genetic relationship among materials, and the glutinous sorghum germplasm showed abundant genetic diversities.

参考文献/References:

[1]新楠，罗峰，李贺，等．播期对天津地区甜高粱主要性状的影响[J]．江苏农业科学，2014，42(8)：90-92.
[2]郭晓丽，时丽冉，王广才，等.干旱胁迫对不同高粱品种生理特性的影响[J]．江苏农业科学，2014，42(2)：91-93.
[3]董玉琛. 生物多样性及作物遗传多样性检测[J].作物品种资源，1995（3）：1-5.
[4]MENZ M A, KLEIN R R, UNRUH N C, et al. Genetic diversity of public inbreds of sorghum determined by mapped AFLP and SSR markers[J]. Crop Sci, 2004, 44(4): 1236-1244.
[5]MENZ M A, KLEIN R R, MULLET J E, et al. A high-density genetic map of Sorghum bicolor (L.) Moench based on 2926 AFLP, RFLP, and SSR markers[J]. Plant Mol Biol, 2002, 48(5-6): 483-499.
[6]徐吉臣, WEERASURIYA Y M, BENNETZEN J L. 高梁(Sorghum bicolor)分子图谱的构建及寄生草(Striga asiatica)萌发诱导物基因的定位[J].遗传学报，2001，28（9）: 870-876.
[7]ARUNA C, BHAGWAT V R, MADHUSUDHANA R, et al. Identification and validation of genomic regions that affect shoot fly resistance in sorghum [Sorghum bicolor (L.) Moench][J]. Theor Appl Genet, 2011, 122 (8): 1617-1630.
[8]JORDAN D R, EMMA S, MACE R G, et al. Molecular mapping and candidate gene identification of the Rf2 gene for pollen fertility restoration in sorghum[Sorghum bicolor (L.) Moench][J]. Theor Appl Genet, 2010, 120(1):1279-1287.
[9]王黎明，焦少杰，姜艳喜，等.142份甜高粱品种的分子身份证构建[J].作物学报，2011，7（11）：1975-1983.
[10]ALI M L, RAJEWSKI J F, BAENZIGER P S, et al. Assessment of genetic diversity and relationship among a collection of US sweet sorghum germplasm by SSR markers[J]. Mol Breeding,2008,21: 497-509.
[11]赵香娜，岳美琪，刘洋，等.国内外甜高梁种质遗传多样性的SSR分析[J].植物遗传资源学报，2010，11（4）：407-412.
[12]冯国郡，叶凯，李桂英，等.新疆甜高粱种质资源遗传多样性的SSR分析[J].植物遗传资源学报，2012，13（4）：549-554.
[13]余传涨，翟国伟，邹桂花，等. 41个高粱品种遗传多样性的SSR标记检测[J].江苏农业学报, 2010，26（2）：248-253.
[14]FANG X E, CHEN Q, YIN L P, et al. Application of ISSR in genetic relationship analysis of sorghum species[J]. Acta Agron Sin, 2008,34(8):1480-1483.
[15]ZHANG H, WANG J C, WANG D J, et al.Assessment of genetic diversity in Chinese sorghum landraces using SSR markers as compared with foreign accessions[J]. Acta Agronomica Sinica, 2011,37(2):224-234.
[16]SAMBROOK J, FRITSCH E F, MANIATIS T. Molecular cloning a laboratory manual[M]. Third edition. New York:Cold Spring Habour Laboratory Press, 1989.
[17]SMITH J S C, CHIN E C L, SHU H, et al. An evolution of the utility of SSR loci as molecular markers in maize (Zea mays L.): comparisons with data from RFLPs and pedigree[J].Theoretical and Applied Genetics,1997,95: 163-173.
[18]KIMURA M, OHTA T. Mutation and evolution at the molecular lever[J].Genetics,1973,73(Suppl): 19-35.
[19]ROLHF F J. NTSYS-pc version 1.80[CP/DK]. Setauket, NY:Distribution by Exeter Software, 1993. 
[20]高彦东， 陈悦. 42份高粱亲本的聚类分析[J].杂粮作物，2001，21（3）：14-15.
[21]赵香娜，李桂英，刘洋，等. 国内外甜高粱种质资源主要性状遗传多样性及相关性分析[J].植物遗传资源学报，2008，9（3）：302-307.
[22]徐影，詹秋文，董书军，等. 20份甜高粱遗传多态性的RAPD和SSR分析[J].华北农学报，2013，28（1）：12-18.
[23]岳文娣，魏利斌，张体德. 芝麻种质资源SSR标记遗传多样性与群体结构分析[J].作物学报，2012，38（12）：2286-2296.

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备注/Memo

备注/Memo:
收稿日期：2014-05-14 基金项目：四川省农业科学院青年基金项目(2012QNJJ-023);国家高粱产业技术体系酿酒(糯)高粱新品种选育项目(CARS-06-01-05);四川省财政育种基因工程项目 (2011JYGC11-031);四川省农作物育种攻关项目(2011NZ0098-6) 作者简介：倪先林(1981-)，男，四川乐山人，硕士，助理研究员，主要从事高粱相关研究工作。（Tel）13548366866；（E-mai）xianlinn@163.com 通讯作者：丁国祥，（E-mail）dgx6132@163.com
更新日期/Last Update: 2015-02-28