[1]周丽慧,张亚东,朱镇,等.利用染色体片段置换系群体检测水稻产量相关性状QTL[J].江苏农业学报,2015,(01):1-9.[doi:10.3969/j.issn.1000-4440.2015.01.001]
　ZHOU Li-hui,ZHANG Ya-dong,ZHU Zhen,et al.Quantitative trait locus (QTL) detection for rice yield-related traits using chromosome segment substitution lines[J].,2015,(01):1-9.[doi:10.3969/j.issn.1000-4440.2015.01.001]
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利用染色体片段置换系群体检测水稻产量相关性状QTL()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年01期
页码:
1-9
栏目:
遗传育种·耕作栽培·生理生化
出版日期:
2015-02-28

文章信息/Info

Title:
Quantitative trait locus (QTL) detection for rice yield-related traits using chromosome segment substitution lines
作者:
周丽慧; 张亚东; 朱镇; 陈涛; 赵庆勇; 姚姝; 赵凌; 赵春芳; 于新; 王才林
（江苏省农业科学院粮食作物研究所/江苏省优质水稻工程技术研究中心/国家水稻改良中心南京分中心，江苏南京210014）
Author(s):
ZHOU Li-hui; ZHANG Ya-dong; ZHU Zhen; CHEN Tao; ZHAO Qing-yong; YAO Shu; ZHAO Ling; ZHAO Chun-fang; YU Xin; WANG Cai-lin
（Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu High Quality Rice Research and Development Center/ Nanjing Branch of Chinese National Center for Rice Improvement, Nanjing 210014, China）
关键词:
水稻; 染色体片段置换系; 数量性状基因座; 产量
Keywords:
rice; chromosome segment substituted line(CSSL); quantitative trait locus(QTL); yield
分类号:
S511.03
DOI:
10.3969/j.issn.1000-4440.2015.01.001
文献标志码:
A
摘要:
为了发掘单株产量及其构成因素相关性状的数量性状基因座（QTL），本研究以9311/日本晴染色体片段置换系群体为材料，调查了单株产量及单株产量构成因素(单株实粒数、单株总颖花数、结实率、每穗实粒数、有效穗数、每穗颖花数、千粒质量等8个性状)。利用IciMapping v3.1软件，将分子标记检测结果与田间性状调查值相结合，定位了与单株产量、单株实粒数、千粒质量、每穗颖花数、结实率、单株总颖花数6个性状有关的QTL，未定位到与有效穗数(EPN)和每穗实粒数(GPP)有关的QTL。共定位到的10个相关QTLs，分别分布于第1条、第2条、第5条、第7条、第8条染色体的7个区间，贡献率为7.52%～44.59%，其中4个QTLs的贡献率大于10.00%。单株产量qGY1，单株实粒数qGN1，结实率qSSR1.2、qSSR2和qSSR8，加性效应值为负值，表明9311的等位基因表现为增效作用；单株总颖花数qSN2，结实率qSSR1.1，千粒质量qTGW5，每穗颖花数qSPP5和qSPP7，加性效应值为正值，表明日本晴的等位基因表现为增效作用。10个QTLs位点中，除qSN2、qTGW5、qSPP5与已克隆的LP、qSW5、OsNADH-GOGAT2可能位于同一区域外，其余7个位点均未被克隆或精细定位。
Abstract:
To identify the quantitative trait locus(QTL) referring to yield and its components for improvement of yield, a population of chromosome segment substitution lines (CSSLs) derived from backcross between indica recipient 9311 and japonica donor Nipponbare were employed, and the yield-related traits, such as grain yield per plant(GY), grain number per plant(GN), spikelet number per plant(SN), seed setting rate(SSR), grain number per panicle(GPP), effective panicles number(EPN),spikelet number per panicle(SPP), and 1 000-grain weight(TGW) were measured. A total of ten QTLs for the yield-related traits except for EPN and GPP were located at seven regions on chromosomes 1, 2, 5, 7 and 8, with explained phenotypic variations (EPV) ranging from 7.52% to 44.59%, four of which with EPV above 10.00%. qGY1, qGN1, qSSR1.2, qSSR2 and qSSR8 derived from 9311 allele showed negative effect, while the others, including qSN2, qSSR1.1, qTGW5, qSPP5 and qSPP7 derived from Nipponbare allele, exhibited positive effect. Three QTLs, qSN2, qTGW5, qSPP5 were located on the same regions as the reported cloned loci LP, qSW5, OsNADH-GOGAT2, respectively, and the rest seven QTLs were not cloned or fine mapped.

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备注/Memo

备注/Memo:
收稿日期：2014-07-28 基金项目：国家水稻产业技术体系项目（CARS-01-47）；江苏省农业科技自主创新基金项目[CX（11）1022] 作者简介：周丽慧（1981-），女，湖南沅江人，硕士，助理研究员，主要从事水稻遗传育种研究。(Tel)13645176538;（E-mail）zhoulihui@jaas.ac.cn 通讯作者：王才林，（E-mail）clwang@jaas.ac.cn
更新日期/Last Update: 2015-02-28