[1]徐颖超,张思程,薛舒丹,等.南瓜叶黄素基因紧密连锁的InDel分子标记开发及应用[J].江苏农业学报,2024,(02):348-358.[doi:doi:10.3969/j.issn.1000-4440.2024.02.017]
 XU Ying-chao,ZHANG Si-cheng,XUE Shu-dan,et al.Development and application of closely linked InDel molecular markers of lutein gene in Cucurbita moschata Duch.[J].,2024,(02):348-358.[doi:doi:10.3969/j.issn.1000-4440.2024.02.017]
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南瓜叶黄素基因紧密连锁的InDel分子标记开发及应用()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年02期
页码:
348-358
栏目:
园艺
出版日期:
2024-02-25

文章信息/Info

Title:
Development and application of closely linked InDel molecular markers of lutein gene in Cucurbita moschata Duch.
作者:
徐颖超1张思程12薛舒丹1刘佳黎1朱吉童1孟琦涛1林惠境1聂呈荣2钟玉娟1
(1.广东省农业科学院蔬菜研究所/广东省蔬菜新技术研究重点实验室,广东广州510640;2.佛山科学技术学院园艺学院,广东佛山528231)
Author(s):
XU Ying-chao1ZHANG Si-cheng12XUE Shu-dan1LIU Jia-li1ZHU Ji-tong1MENG Qi-tao1LIN Hui-jing1NIE Cheng-rong2ZHONG Yu-juan1
(1.Vegetable Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou 510640, China;2.School of Horticulture, Foshan University of Science and Technology, Foshan 528231, China)
关键词:
南瓜叶黄素QTL定位HPLC测定InDel分子标记
Keywords:
pumpkinluteinQTL localizationHPLC determinationInDel molecular marker
分类号:
S642.1
DOI:
doi:10.3969/j.issn.1000-4440.2024.02.017
摘要:
本研究旨在开发与中国南瓜(Cucurbita moschata Duch.)叶黄素含量的数量性状基因座(Quantitative trait locus,QTL)紧密连锁且实用性强的分子标记,以加速南瓜的育种进程。前期在F2代群体中定位到与叶黄素含量紧密连锁的主效QTL位点qlut11-a,在其两侧翼分子标记R1_38695和R2_55819之间开发了8对InDel分子标记。通过对F2代群体及部分高代(F8代)重组自交系株系进行基因型和叶黄素表型分析,明确了InDel分子标记G005310和G005670可有效筛选高叶黄素含量和低叶黄素含量材料,并在近等基因系构建中证实了其能用于创制高叶黄素含量的南瓜种质,BC5F1果实中的最高叶黄素含量约是低叶黄素含量亲本果实含量的2.8倍,且占高叶黄素含量亲本果实的96%。本研究结果为加速南瓜高叶黄素含量种质育种进程提供了更实用的分子标记。
Abstract:
To accelerate the breeding progress of Cucurbita moschata Duch. (C. moschata), the study aimed to develop molecular markers which are tightly linked with quantitative trait locus (QTL) of lutein content in C. moschata and are highly practicable. Eight pairs of insertion-deletion (InDel) molecular markers were developed between the two flanking molecular markers R1_38695 and R2_58819, based on the previous localization of the primary QTL locus qlut11-a which was tightly linked to lutein content in F2 population. Genotype analysis and phenotypic analysis of lutein content for the F2 population and recombinant inbreed lines (RILs) in part of the high generation (F8) revealed that, InDel molecular markers G005310 and G005670 could be used to effectively screen materials with high and low lutein contents. Furthermore, the two InDel molecular markers were proved to be able to culture pumpkin germplasms with high lutein content in the construction of near isogenic line (NIL). The highest lutein content in BC5. F1 fruit was about 2.8 times as high as the lutein content in parent fruit with low lutein content, and the lutein content in BC5F1 fruit accounted for about 96% of the lutein content in parent fruit with high lutein content. The research results can provide more practical molecular markers for accelerating breeding process of pumpkin germplasms with high lutein content.

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备注/Memo

备注/Memo:
收稿日期:2023-04-17基金项目:广东省农业科学院食品营养与健康研究中心建设运行经费项目(XTXM202205);广西重点研发计划项目(桂科AB23026076);广东省农业科学院科技人才引进专项(R2022YJ-YB3012);广东省蔬菜新技术重点实验室开放基金项目(202201);国家自然科学基金项目(32172604);广东省现代农业产业技术体系创新团队建设项目(2022KJ117)作者简介:徐颖超(1994-),女,山东烟台人,博士,助理研究员,研究方向为蔬菜品质遗传改良及代谢机理。(E-mail)xuyingchao@gdaas.cn通讯作者:钟玉娟,(E-mail)zhongyujuan@gdaas.cn;聂呈荣,(E-mail)niecr@126.com
更新日期/Last Update: 2024-03-17