[1]李灵慧,吴然然,陈景斌,等.基于PARMS技术的绿豆抗叶斑病基因VrTAF5分子标记的开发[J].江苏农业学报,2021,(06):1386-1392.[doi:doi:10.3969/j.issn.1000-4440.2021.05.004]
　LI Ling-hui,WU Ran-ran,CHEN Jing-bin,et al.Development of molecular markers of mung bean leaf spot disease resistance gene VrTAF5 based on PARMS technology[J].,2021,(06):1386-1392.[doi:doi:10.3969/j.issn.1000-4440.2021.05.004]
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基于PARMS技术的绿豆抗叶斑病基因VrTAF5分子标记的开发()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年06期
页码:
1386-1392
栏目:
遗传育种·生理生化
出版日期:
2021-12-30

文章信息/Info

Title:
Development of molecular markers of mung bean leaf spot disease resistance gene VrTAF5 based on PARMS technology
作者:
李灵慧1; 2; 吴然然2; 陈景斌2; 崔晓艳2; 袁星星2; 朱月林1; 陈新2
（1.南京农业大学园艺学院，江苏南京210095；2.江苏省农业科学院经济作物研究所，江苏南京210014）
Author(s):
LI Ling-hui1; 2; WU Ran-ran2; CHEN Jing-bin2; CUI Xiao-yan2; YUAN Xing-xing2; ZHU Yue-lin1; CHEN Xin2
(1.College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;2.Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
绿豆; 叶斑病; VrTAF5基因; SNP分子标记; PARMS技术
Keywords:
mung bean; Cercospora leaf spot disease; VrTAF5 gene; SNP molecular marker; PARMS technology
分类号:
S522.035.3
DOI:
doi:10.3969/j.issn.1000-4440.2021.05.004
文献标志码:
A
摘要:
叶斑病是绿豆生产上主要的真菌性病害，利用抗性品种是防治该病害最经济、安全、有效的途径。为筛选抗病资源，根据抗性基因VrTAF5的突变位点,利用五引物扩增受阻突变体系（Penta-primer amplification refractory mutation system，PARMS）开发分子标记。并利用抗病性具有差异的164份绿豆资源对所开发的分子标记的可靠性进行检验和评价。结果表明，VrTAF5-1517分子标记在7个分子标记中的检出率最高，基因分型为AA、AC、CC，可以有效区分基因碱基序列的SNP差异。根据叶斑病抗性评价结果，该标记对抗病材料的选择效率为75.00%、感病材料的选择效率为93.42%。VrTAF5-1517分子标记对抗病资源中检出抗病基因型的比例、对高感和感病资源中检出感病基因型比例均为100%，说明本研究开发的分子标记可用于绿豆抗叶斑病分子标记辅助育种。
Abstract:
Cercospora leaf spot (CLS) disease is the main fungal disease in mung bean production. Using resistant varieties is the most economical, safest and effective way to control CLS disease. For selection of disease-resistant resources, molecular markers were developed based on the mutation sites of the resistance gene VrTAF5 by penta-primer amplification refractory mutation system (PARMS) technology. In addition, 164 mung bean resources with different disease resistance were used to test and evaluate the reliability of the developed molecular markers. The results showed that the detection rate of VrTAF5-1517 was the highest among the seven molecular markers, and the genotypes were AA, AC and CC. Moreover, SNP differences in gene base sequences could be effectively distonguished. According to the evaluation of leaf spot resistance, the selection efficiency of VrTAF5-1517 for resistant and susceptible accessions was 75% and 93.42%, respectively. The proportions of resistant genotypes detected in resistant resources and susceptoble genotypes detected in highly susceptible and susceptible resources by VrTAF5-1517 were 100%, suggestingt that the molecular markers developed in this study could be used in molecular marker-assisted breeding for resistance to CLS disease of mung bean.

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备注/Memo

备注/Memo:
收稿日期：2020-03-25基金项目：国家重点研发计划项目（2019YFD1001301、2019YFD1001300）；国家食用豆产业技术体系生物防治与综合防控岗位科学家项目（CARS-08-G15）；江苏特粮特经产业技术体系集成创新中心项目[JATS(2019)399]；江苏高校优势学科建设工程资助项目作者简介：李灵慧（1995-），男，山东德州人，硕士研究生，从事植物分子育种研究。（E-mail）2582355776@qq.com通讯作者：朱月林，（E-mail）ylzhu@njau.edu.cn；陈新，（E-mail）cx@jaas.ac.cn
更新日期/Last Update: 2022-01-07