[1]潘孝青,王杏龙,杨杰,等.不同LED光色对家兔繁殖性能的影响及其分子调控机制[J].江苏农业学报,2020,(01):136-146.[doi:doi:10.3969/j.issn.1000-4440.2020.01.019]
　PAN Xiao-qing,WANG Xing-long,YANG Jie,et al.Effects of different LED light colors on reproductive performance of rabbits and their molecular regulation mechanism[J].,2020,(01):136-146.[doi:doi:10.3969/j.issn.1000-4440.2020.01.019]
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不同LED光色对家兔繁殖性能的影响及其分子调控机制()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年01期
页码:
136-146
栏目:
畜牧兽医·水产养殖
出版日期:
2020-02-29

文章信息/Info

Title:
Effects of different LED light colors on reproductive performance of rabbits and their molecular regulation mechanism
作者:
潘孝青1; 2; 王杏龙1; 杨杰2; 秦枫2; 邵乐2; 李健2; 张霞2; 翟频2
(1.扬州大学动物科学与技术学院，江苏扬州212400；2.江苏省农业科学院畜牧研究所，江苏南京210014)
Author(s):
PAN Xiao-qing1; 2; WANG Xing-long1; YANG Jie2; QIN Feng2; SHAO Le2; LI Jian2; ZHANG Xia2; ZHAI Pin2
(1.College of Animal Science and Technology, Yangzhou University, Yangzhou 212400, China;2.Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
LED光; 兔; 繁殖性能; 分子调控
Keywords:
LED light color; rabbit; reproductive performance; molecular regulation
分类号:
S829.14+6
DOI:
doi:10.3969/j.issn.1000-4440.2020.01.019
文献标志码:
A
摘要:
为探讨不同LED光色影响母兔繁殖效率的分子机制，将单笼饲养的300只后备母兔随机分成5组进行不同LED光色处理，每组3个重复，每个重复20只。在4个不同LED光色处理组兔笼上方60 cm处向下分别投射LED红光（660 nm）、LED绿光（540 nm）、LED蓝光（480 nm）和LED白光（400～760 nm），对照组投射白炽灯光（400～1 050 nm）。母兔每周接受3次补光（LED光处理），即每周的周五至周日下午18∶00至次日上午6∶00，时长为12 h，预试验7 d，正试期90 d。LED灯及白炽灯的光照度均设置为100 lx。结果显示：LED红光组母兔同期发情率与白炽灯光对照组及LED白光组相比，分别提高20.89个百分点和10.51个百分点，差异显著（P<0.05）；与绿光组及蓝光组相比，分别提高4.66个百分点和7.33个百分点，差异不显著（P>0.05）。LED红光组母兔卵巢红色大卵泡数量有增加趋势，但各试验组间卵巢红色大卵泡数量差异不显著（P>0.05）。与白炽灯光、LED白光、绿光、蓝光相比，LED红光能促进母兔垂体GnRHR mRNA表达，但差异不显著（P>0.05）；与LED白光、绿光、蓝光相比，LED红光显著提高了卵巢组织中FSHR、LHR 基因mRNA的表达（P<0.05）；与白炽灯光对照相比，LED红光有提高卵巢组织中FSHR、LHR 基因mRNA表达水平的趋势，但差异不显著（P>0.05）。在原始卵泡（PrF）阶段与初级卵泡（PF）阶段，不同LED光色处理的卵泡数量差异不显著（P>0.05）；与原始卵泡（PrF）及初级卵泡（PF）相比，由于卵泡逐级闭锁，次级卵泡（SF）数量显著降低，不同组间开始出现差异，与LED绿光组及LED蓝光组相比，LED红光组、LED白光组及白炽灯光对照组次级卵泡（SF）数量显著增加（P<0.05）；由于卵泡进一步闭锁，与原始卵泡（PrF）、初级卵泡（PF）以及次级卵泡（SF）相比，三级卵泡（TF）数量显著减少，不同组间三级卵泡（TF）数量也呈现不同差异，与LED绿光组、LED蓝光组及白炽灯光对照组相比，LED红光组三级卵泡（TF）数量显著增加（P<0.05），但与LED白光组相比，差异不显著（P>0.05）。与LED绿光相比，LED红光及白炽灯光能显著抑制母兔卵巢FoxO1 mRNA表达（P<0.05）；与LED白光及LED蓝光相比，LED红光及白炽灯光有抑制母兔卵巢FoxO1 mRNA表达的趋势，但差异不显著（P>0.05）。Bcl-2/Bax mRNA两个凋亡相关基因在兔卵巢中均有表达，但两者的相对表达量显著差异（P<0.05），Bax mRNA表达量显著高于Bcl-2 mRNA表达量；不同LED光色对兔卵巢Bcl-2/Bax mRNA表达的影响也存在差异，与LED绿光、蓝光及白炽灯光相比，LED红光显著提高Bcl-2 mRNA的表达量（P<0.05），但与LED白光相比差异不显著（P>0.05），Bcl-2基因表达量受到不同光色的影响呈现红光>白光>白炽灯光>绿光>蓝光；不同LED光色对Bax mRNA表达的影响呈现绿光>蓝光>白光>白炽灯光>红光，但各组间差异不显著（P>0.05）。总之，规模兔场短日照季节周期化繁殖生产中，每周连续3 d于晚间18∶00至次日凌晨6∶00之间，采用100 lx的LED红光进行补光，可提高母兔同期发情率；HPG轴上繁殖调控关键受体基因转录水平、卵巢次级卵泡和三级卵泡数量以及与卵泡闭锁和氧化应激相关基因转录水平均受到LED不同光色的影响。
Abstract:
To explore the molecular mechanism of different LED light colors affecting the reproductive efficiency of female rabbits, three hundred female rabbits reared in a single cage were randomly divided into five groups for different LED light colors treatments. LED red light (660 nm), LED green light (540 nm), LED blue light (480 nm) and LED white light (400-760 nm) were projected downwards at 60 cm above the rabbit cage. The control group was incandescent lamp (400-1 050 nm). Female rabbits received the treatment with supplementary light three times a week, the duration was 12 hours (12D∶12L), the pre-experiment was seven days, and the standard test was 90 days. The light intensity of LED lamp and incandescent lamp was set as 100 lx. Compared with incandescent lamp group and LED white light group, the estrus rate of female rabbits in LED red light group increased by 20.89 percentage points and 10.51 percentage points（P<0.05）. Compared with LED green light group and LED blue light group, the estrus rate of female rabbits in LED red light group increased by 4.66 percentage points and 7.33 percentage points（P>0.05）. The number of large red follicles in the ovaries of the female rabbits in the LED red light group increased, but there was no significant difference between the experimental groups （P>0.05）. Compared with incondescent light, LED white light, LED green light and LED blue light, LED red light could promote the mRNA expression of GnRHR in the pituitary gland of female rabbits, but the difference was not significant （P>0.05）. Compared with LED white light, LED green light and LED blue light, LED red light significantly increased the expression of FSHR and LHR in ovarian tissue （P<0.05）. Compared with the incandescent light, LED red light could increase mRNA expression of FSHR and LHR gene in ovarian tissue, but the difference was not significant （P>0.05）. In the primordial follicles (PrF) stage and the primary follicles (PF) stage, the effect of different LED light colors on the number of follicles was not significant（P>0.05）. Compared with PrF and PF, the number of secondary follicles (SF) was significantly reduced due to the stepwise blocking of follicles, and differences began to appear between different groups. Compared with that in LED green light group and the LED blue light group, the number of SF in LED red light group, LED white light group and incandescent light group increased significantly （P<0.05）. Due to further atresia of follicles, the number of tertiary follicles (TF) decreased significantly. Compared with that in the LED green light group, LED blue light group and incandescent light group, the number of TF in the LED red light group was significantly increased （P<0.05）, but there was no significant difference in the number of TF between the LED red light group and the LED white light group（P>0.05）. Compared with the LED green light, LED red light and incandescent light could significantly inhibit mRNA expression of FoxO1 in female rabbit ovaries （P<0.05）. Compared with LED white light and LED blue light, LED red light and incandescent light could inhibit mRNA expression of FoxO1 in female rabbit ovary, but the difference was not significant （P>0.05）. Both apoptosis-related genes of Bcl-2/Bax mRNA were expressed in rabbit ovary, but the relative expression levels of them were significantly different （P<0.05）. The expression level of Bax mRNA was significantly higher than that of Bcl-2 mRNA. The effects of different LED light colors on Bcl-2/Bax mRNA expression in rabbit ovaries were also different. Compared with the LED green light, LED blue light and incandescent light, LED red light significantly increased the expression of Bcl-2 mRNA （P<0.05）, but there was no significant difference in the expression of Bcl-2 mRNA between the LED red light group and LED white light group（P>0.05）. The expression of Bcl-2 gene was affected by different light colors, showing a trend of red light> white light> incandescent light> green light> blue light. The effect of LED light color on Bax mRNA expression showed a trend of green light> blue light> white light> incandescent light> red light, but there was no significant difference （P>0.05）. In conclusion, during the periodical breeding and production of large-scale rabbit farms in short-day season, the estrus rate of female rabbits can be improved by using 100 lx LED red light to supplement light from 18∶00 in the evening to 6∶00 in the morning of the next day for three days in a week. The transcription levels of key receptor genes on the HPG axis for regulation of reproduction, the number of secondary follicles, the number of tertiary follicles, and the transcription levels of genes related to follicular atresia and oxidative stress were affected by different LED light colors.

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备注/Memo

备注/Memo:
收稿日期：2019-10-10 基金项目：江苏省农业科学院探索性颠覆性创新计划项目[（17）2012]；国家兔产业技术体系-南京综合试验站项目（CARS-43-G-2）；国家科技基础条件平台-特种经济动物种质资源平台项目（TZDWZYPT2019-02） 作者简介：潘孝青（1983-），男，江苏句容人，硕士，副研究员，从事畜禽生态健康养殖技术研究。（E-mail）176169314@qq.com 通讯作者：王杏龙，（E-mail）xinglongwang@yzu.edu.cn
更新日期/Last Update: 2020-03-13