[1]辛松林,秦文,孙传红,等.腐皮镰孢霉菌侵染及保鲜剂处理对秋葵相关抗性酶的影响[J].江苏农业学报,2018,(05):1161-1168.[doi:doi:10.3969/j.issn.1000-4440.2018.05.027]
 XIN Song-lin,QIN Wen,SUN Chuan-hong,et al.Effects of Fusarium solani infection and preservative treatment on resistance associated enzyme from okra[J].,2018,(05):1161-1168.[doi:doi:10.3969/j.issn.1000-4440.2018.05.027]
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腐皮镰孢霉菌侵染及保鲜剂处理对秋葵相关抗性酶的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年05期
页码:
1161-1168
栏目:
加工贮藏·质量安全
出版日期:
2018-10-25

文章信息/Info

Title:
Effects of Fusarium solani infection and preservative treatment on resistance associated enzyme from okra
作者:
辛松林12秦文1孙传红2徐丹2黄倩2毛美丹2方子豪2
(1.四川农业大学食品学院,四川雅安625014;2.四川旅游学院食品学院,四川成都610100)
Author(s):
XIN Song-lin12QIN Wen11SUN Chuan-hong2XU Dan2HUANG Qian2MAO Mei-dan2FANG Zi-hao2
(1. College of Food Science, Sichuan Agricultural University, Ya’an 625014, China;2.College of Food Science, Sichuan Tourism College, Chengdu 610100, China)
关键词:
川秋葵腐皮链孢霉菌保鲜剂抗性酶
Keywords:
Sichuan okraFusarium solanipreservativesresistant enzyme
分类号:
S649
DOI:
doi:10.3969/j.issn.1000-4440.2018.05.027
文献标志码:
A
摘要:
为研究秋葵果实响应外界胁迫反应及合理利用果实抗性酶的保护功能,以川秋葵为试验材料,采用人工接种腐皮链孢霉菌和保鲜剂处理的方法,研究秋葵果实抗性酶的变化。结果表明,POD、PPO、PAL、C4H、4CL、CAD和TAL参与了秋葵果实响应腐皮链孢霉菌侵染和成熟衰老的反应过程。4CL、POD、PPO在整个腐皮链孢霉菌侵染阶段活性持续增高起着重要的防御作用;PAL、CAD在腐皮链孢霉菌侵染的4 d内活性增高,且在整个侵染阶段都保持了较高的活性;C4H在腐皮链孢霉菌侵染的3 d内起着重要的防御作用;TAL在侵染24 h迅速启动响应反应。不同保鲜剂对抗性酶活性表达的诱导作用不同,1-MCP与壳聚糖复合处理对维持果实抗病性和采后品质的效果最好。
Abstract:
In order to study the response of okra fruits to the external stress and the protection of fruit resistant enzyme, the changes of resistant enzymes in okra fruits were studied by artificially inoculating Fusarium solani and preservative treatment methods. The results showed that POD, PPO, PAL, C4H, 4CL, CAD and TAL were involved in the response of okra fruits to the ripening and senescence and infection of Fusarium solani. 4CL, POD and PPO played an important role in the defense of the constantly increasing activity during the infection stage of Fusarium solani. The activities of PAL and CAD increased within four days of the inoculation of Fusarium solani and maintained the relatively high activity throughout the infection stage. C4H played an important defense role within three days of the infection by Fusarium solani. The response of TAL was activated rapidly within 24 hours of infection. However, different preservatives had different induction effects on the active expression of resistant enzyme. The combination of 1-MCP and chitosan was best for maintaining the disease resistance of fruits and postharvest quality.

参考文献/References:

[1]刘招龙,张绍铃,孙益林.梨叶片感染轮纹病菌后的生理变化[J].植物保护,2007,32(6):78-80.
[2]闫媛媛,胡文忠,姜爱丽,等.茉莉酸甲酯和乙烯利处理对鲜切富士苹果抗氧化酶活力和苯丙烷代谢的影响[J].食品工业科技,2015,36(16):324-327,332.
[3]林晓姿,何志刚,李维新,等.外源乙烯胁迫对枇杷果实若干品质及PPO活性的影响[J].福建农业学报,2009,24(5): 467- 470.
[4]辛松林,焦露,徐晓雪,等.川秋葵1号采后菌核病病原菌鉴定及其生物学特性研究[J].食品工业科技,2017,12(38):186-190
[5]郑建秋,师迎春,胡铁军. 蔬菜菌核病的识别与防治[J]. 中国蔬菜,2005,1(2):53-54.
[6]曹福祥,戚佩坤. 腐皮镰孢霉的现代分类学[J]. 华南农业大学学报,1991, 2:66-73.
[7]张慧琴,毛雪琴,肖金平,等.猕猴桃溃疡病病原菌分子鉴定与抗性材料初选[J].核农学报,2014,28(7):1181-1187.
[8]LIU H X, JIANG W B, BI Y, et al. Postharvest BTH treatment induces resistance of peach (Prunus persica L. cv. Jiubao) fruit to infection by Penicillium expansum and enhances activity of fruit defense mechanisms[J]. Postharvest Biology and Technology, 2005, 35(3):263-269.
[9]朱明华,欧阳光察,薛应龙. 黄瓜免疫诱导过程中 G6PD、PAL、4CL、PO 活性和木质素含量的变化[J]. 上海农业学报,1990,6(2): 21-26.
[10]LAMB C J, RUBERY P H. A spectrophotometric assay for trans-cinnamic acid 4-hydroxylase activity[J]. Analytical Biochemistry, 1975, 68(2):554-561.
[11]MORRISON T A, KESSLER J R, HATFIELD R D, et al. Activity of two lignin biosynthesis enzymes during development of a maize internode[J]. Journal of the Science of Food and Agriculture, 1994, 65(2): 133-139.
[12]WAJAHATULLAH K, B PRITHIVIRAJ, DL SMITH. Photosynthetic response of corn and soybean, to foliar application of Salicyates[J]. Journal of Plant Physiology, 2003, 160(5): 485-92.
[13]JIANG A L, TIAN S P, XU Y. Effect of CA with high-O2 or high-CO2 concentrations on postharvest physiology and storability of sweet cherry[J]. Acta Botanic Sinica, 2002, 44 (8): 925-930.
[14]CLAIRBONE A. CaTALase activity. In: Greenwald W.A. (ed.) Handbook of methods of oxygen radical research[M]. Boca Raton:CRC press, 1985:283-284.
[15]MAUCH-MANI B, SLUSARENKO A J. Production of salicylic acid precursors is a major function of phenylalanine ammonia-lyase in the resistance of Arabidopsis to Peronosporaparasitica[J]. The Plant Cell, 1996, 8(2): 203-212.
[16]WHETTEN R, SEDEROFF R. Lignin biosynthesis[J]. The Plant Cell, 1995, 7(7): 1001-1013.
[17]李美芹. 壳聚糖抑制番茄叶霉病菌的活性与诱导抗性及其机理研究[D].青岛:中国海洋大学,2007.
[18]曾永三,王振中.苯丙氨酸解氨酶在植物抗病反应中的作用[J].仲恺农业技术学院学报,1999,12(3): 56-65.
[19]YOU Y L, JIANG Y M, SUN J, et al. Effects of short-term anoxia treatment on browning of fresh-cut Chinese water chestnut in relation to antioxidant activity[J]. Food Chemistry, 2012,132(3):1191-1196.
[20]魏敏,周会玲,陈小利,等.低温贮藏对鲜切富士苹果褐变的影响[J].西北农林学院学报, 2011, 26(5) : 131-134 .
[21]REIMERS P J, LEACH J E. Race-specific resistance to Xanthomonas oryzae pv. Oryzae conferred by bacterial blight resistance gene Xa-10 in rice (Orzya sativa) involves accumulation of a lignin-like substance in host tissues[J]. Molecular Plant Pathology, 1991, 38(1): 39-55.
[22]李莉,赵越,马君兰.苯丙氨酸代谢途径关键酶: PAL、C4H、4CL 研究新进展[J].生物信息学, 2007, 5(4): 187-189.
[23]张树武,徐秉良,刘佳等.白粉病菌侵染对美洲南瓜不同抗性品种PAL基因表达量的影响[J].核农学报,2015,29(10):1876-1883.
[24]张峰,曾化伟,廖祥儒,等. 微生物酪氨酸解氨酶的研究进展[J]. 食品与生物技术学报,2010,29(4):496-499.
[25]DIXON R A,ACHNINE L,KOTA P,et al. The phenylpropanoidpathway and plant defence: a genomics perspective[J]. MolecularPlant Pathology,2002,3(5):371-390.
[26]欧阳光察,薛应龙.植物苯丙烷类代谢的生理意义及其调控[J].植物生理学通讯,2012,24(3):9-16.
[27]贺立红,张进标,宾金华,等.苯丙氨酸解氨酶的研究进展[J].食品科技,2006,31(7):31-34.
[28]刘筱,潘静宇,李永才,等. 寡雄蛋白(Oligandrin)处理对马铃薯块茎干腐病及苯丙烷代谢的影响[J]. 食品工业科技,2017,38(6):339-344,369.
[29]郭宇欢,张丽媛,何玲,等. 银杏叶粗提物对猕猴桃灰葡萄孢霉的抑制[J]. 现代食品科技,2017(6):111-117.
[30]赵亚婷,朱璇,马玄,等. 采前水杨酸处理对杏果实抗病性及苯丙烷代谢的诱导[J]. 食品科学,2015,36(2):216-220.
[31]范存斐,毕阳,王云飞,等. 水杨酸对厚皮甜瓜采后病害及苯丙烷代谢的影响[J]. 中国农业科学,2012,45(3):584-589.

备注/Memo

备注/Memo:
收稿日期:2017-11-04 基金项目:四川省科技厅应用基础计划项目(2016JY0119);四川省教育厅自然科学重点项目(18ZA0367);四川省大学生创新创业计划项目(201611552053) 作者简介:辛松林(1981-),男,辽宁大连人,博士研究生,副研究员,从事农产品加工与贮藏方面的研究。(E-mail) 23016024@ qq.com 通讯作者:秦文,(E-mail) qinwen1967@ yahoo.com
更新日期/Last Update: 2018-11-05