[1]牛丽影,沈凌雁,刘春菊,等.鲜食糯玉米己醛和己醇的生成与LOX活性及其基因表达的相关性[J].江苏农业学报,2018,(05):1138-1143.[doi:doi:10.3969/j.issn.1000-4440.2018.05.024]
　NIU Li-ying,SHEN Ling-yan,LIU Chun-ju,et al.Correlation of formation of hexanal and hexanol, LOX activity and gene expression in fresh waxy corn[J].,2018,(05):1138-1143.[doi:doi:10.3969/j.issn.1000-4440.2018.05.024]
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鲜食糯玉米己醛和己醇的生成与LOX活性及其基因表达的相关性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年05期
页码:
1138-1143
栏目:
加工贮藏·质量安全
出版日期:
2018-10-25

文章信息/Info

Title:
Correlation of formation of hexanal and hexanol, LOX activity and gene expression in fresh waxy corn
作者:
牛丽影1; 沈凌雁1; 2; 刘春菊1; 李大婧1; 刘春泉1; 肖丽霞2
(1.江苏省农业科学院农产品加工研究所，江苏南京210014；2.扬州大学食品科学与工程学院，江苏扬州225000)
Author(s):
NIU Li-ying1; SHEN Ling-yan1; 2; LIU Chun-ju1; LI Da-jing1; LIU Chun-quan1; XIAO Li-Xia2
(1.Institute of Farm Product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2.College of Food Science and Engineering, Yangzhou University, Yangzhou 225000, China)
关键词:
鲜食糯玉米; 己醛; 脂氧合酶（LOX）; 基因表达量
Keywords:
fresh waxy corn; hexanal; lipoxygenase(LOX); gene expression
分类号:
S513.01
DOI:
doi:10.3969/j.issn.1000-4440.2018.05.024
文献标志码:
A
摘要:
为探讨生鲜糯玉米汁主要挥发性成分己醛与己醇的生成与脂氧合酶（LOX）的关系，对4个籽粒发育期的鲜食糯玉米京甜紫花糯2号和苏玉糯11号生鲜玉米汁中己醛与己醇含量、脂氧合酶活性以及ZmLOX1、ZmLOX2、ZmLOX3、ZmLOX10基因的相对表达量进行了测定和比较。结果显示己醛与己醇含量显著正相关（P<0.05)，4种LOX基因的表达量之间显著正相关（P<0.05)，但LOX活性与其他指标之间未表现出显著的相关关系。主成分分析（PCA）结果显示所有指标聚为3类：4种LOX基因表达量、己醛与己醇含量、LOX活性，三者之间未表现出显著的相关性关系。
Abstract:
To explore the role of lipoxygenase(LOX) in the formation of hexanal and hexanol, the main volatile components in two fresh waxy corn cultivars, Jingtianzihuannuo-2 and Suyunuo-11, at 4 developmental stages were collected. The content of hexanal and hexanol, the activity of LOX, and the relative gene expression of ZmLOX1, ZmLOX2, ZmLOX3, ZmLOX10 were determined. Correlation analysis were performed and the results showed that hexanal content and hexanol content were significantly positive correlated (P<0.05), and there were significantly positive relationships among the expression of four LOX genes (P<0.05)，however, LOX activity didn’t show significant correlation with other parameters. Principal component analysis (PCA) plots showed that all the parameters were clustered into three groups: the expression of four LOX genes, hexanal and hexanol contents, and LOX activity. No significant relationships were found among the three groups.

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备注/Memo

备注/Memo:
收稿日期：2018-01-10 基金项目：江苏省农业科技自主创新基金项目 [CX(17)2013] 作者简介：牛丽影（1977-），女，河北安国人，博士，副研究员，研究方向为果蔬加工与综合利用。(Tel)025-84391570;(E-mail)liying.niu@hotmail.com 通讯作者：肖丽霞，(E-mail)lxxiao@yzu.edu.cn
更新日期/Last Update: 2018-11-05