[1]曹丽萍,杜金梁,贾睿,等.姜黄素、甘草次酸和香菇多糖联合对CCl4诱导的建鲤肝损伤的保护作用[J].江苏农业学报,2018,(05):1100-1106.[doi:doi:10.3969/j.issn.1000-4440.2018.05.019]
　CAO Li-ping,DU Jin-liang,JIA Rui,et al.The liver protective effect of curcumin combined with glycyrrhizic acid and lentinan on CCl4-induced hepatic damage in common carp (Cyprinus carpio)[J].,2018,(05):1100-1106.[doi:doi:10.3969/j.issn.1000-4440.2018.05.019]
点击复制

姜黄素、甘草次酸和香菇多糖联合对CCl4诱导的建鲤肝损伤的保护作用()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年05期
页码:
1100-1106
栏目:
畜牧兽医·水产养殖
出版日期:
2018-10-25

文章信息/Info

Title:
The liver protective effect of curcumin combined with glycyrrhizic acid and lentinan on CCl4-induced hepatic damage in common carp (Cyprinus carpio)
作者:
曹丽萍1; 2; 3;  杜金梁2; 3; 贾睿2; 3; 殷国俊2; 3; Galina Jeney3; 4; 丁炜东2; 高崧1
（1.扬州大学兽医学院，江苏扬州225009；2.中国水产科学研究院淡水渔业研究中心，农业部淡水渔业和种质资源利用重点实验室，江苏无锡214081；3.中国水产科学研究院淡水渔业研究中心，农业部鱼类免疫药理学国际联合实验室，江苏无锡214081；4.Research Institute for Fisheries, Aquaculture and Irrigation, Ann light 8,Szarvas H-4440,Hungary）
Author(s):
CAO Li-ping1; 2; 3; DU Jin-liang2; 3; JIA Rui2; 3; YIN Guo-jun2; 3; GALINA Jeney3; 4; DING Wei-dong2; GAO Song1
（1.Veterinary Medicine College， Yangzhou University， Yangzhou 225009， China；2.Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization， Ministry of Agriculture， Freshwater Fisheries Research Center， Chinese Academy of Fishery Sciences， Wuxi 214081， China；3.International Joint Research Laboratory for Fish immunopharmacology， Chinese Academy of Fishery Sciences， Wuxi 214081， China；4.Research Institute for Fisheries, Aquaculture and Irrigation， Ann light 8， Szarvas H-4440， Hungary）
关键词:
建鲤; 四氯化碳; 肝损伤; 中草药
Keywords:
Cyprinus carpio Jian; CCl4; hepatocyte damage; Chinese herbal medicine
分类号:
S948
DOI:
doi:10.3969/j.issn.1000-4440.2018.05.019
文献标志码:
A
摘要:
以四氯化碳（CCl4）构建建鲤体内急性肝损伤模型，对姜黄素、甘草次酸和香菇多糖联合护肝作用进行研究。分别采用含有姜黄素、甘草次酸、香菇多糖和低、中、高剂量姜黄素+甘草次酸+香菇多糖联合中草药的饲料投喂60 d后，各组建鲤腹腔注射30% CCl4, 注射剂量为每1 g 鱼体注射0.005 ml，禁食72 h，以诱导建鲤急性肝损伤。通过测定各组建鲤生长指标、血清和肝脏生化指标及肝组织炎性细胞因子mRNA的表达情况，观察姜黄素、甘草次酸和香菇多糖联合用药的保肝效果。结果显示：甘草次酸单味用药及中、高剂量姜黄素+甘草次酸+香菇多糖联合用药能显著提高建鲤的相对增重率和特定生长率，显著降低饵料系数；与模型组相比，各用药组能显著抑制CCl4导致的血清中谷丙转氨酶（GPT）、谷草转氨酶（GOT）和乳酸脱氢酶（LDH）活性的升高，显著恢复肝组织中谷胱甘肽（GSH）和超氧化物歧化酶（SOD）水平，抑制丙二醛（MDA）生成，能显著抑制C-Rel、iNOS、IL-1β、TNF-α、IL-6和IL-8表达量的升高；与姜黄素、甘草次酸或香菇多糖单味用药相比较，姜黄素+甘草次酸+香菇多糖联合用药的保肝效果更明显，随着联合用药剂量的升高，作用愈加显著。说明姜黄素、甘草次酸和香菇多糖联合用药，对CCl4诱导的建鲤肝损伤具有协同保护作用。
Abstract:
The Cyprinus carpio Jian acute hepatocyte damage model was constructed by CCl4 induction to evaluate the combined liver protective effect of curcumin, glycyrrhizic acid （GC）and lentinan (LNT). Feeds containing curcumin, glycyrrhizic acid, lentinan and combined groups containing low, medium and high dose of extracts were used to feed carp for 60 d, then 30% CCl4 mixture at 0.005 ml/g was injected intraperitoneally, and acute hepatic injury of carp was induced after fasting for 72 h. Then blood and liver tissue were collected, and growth indicators, biochemical indicators in serum and liver tissue homogenate were determined, respectively. In addition, the changes in mRNA expression level of NF-κB/C-Rel and cytokine IL-1β, that iNOS, TNF-α, IL-6 and IL-8 in tissue were determined using real-time quantitative PCR. The results showed that GC and medium and high dose combined treatments could significantly increase relative weight rate (RWR) and specific growth rate (SGR ), reduce feed conversion ratio (FCR ). Curcumin, GC and LNT and combined group could significantly inhibit the increase of glutamic oxalacetic transaminase (GOT), glutamic pyruvic transaminase (GPT) and lactate dehydrogenase (LDH) contents in carp serum caused by CCl4, promote the recovery of glutathione (GSH) and superoxide dismutase (SOD) levels in liver tissue homogenates and significantly inhibit malondialdehyde (MDA) synthesis. Meanwhile, curcumin, GC and LNT and combined treatments could significantly inhibit the increase of expression of C-Rel, iNOS, IL-1β, TNF-α,IL-6 and IL-8 induced by CCl4. Compared with single drug group, the preventive effect of combined group was more significant with a dose effect. The combination of curcumin, GC and LNT could synergistically reduce the liver injury induced by CCl4.

参考文献/References:

[1]李立，国大亮，朱晓薇，等. 姜黄素类物的提取、分离及精制[J]. 天津中医药，2010，27（6）:509-511.
[2]刘强，张晓燕，鲁颖. 姜黄素对人结肠癌细胞MMP-2、MMP-9表达的影响[J]. 国际医药卫生导报，2009,15（21）:22-24.
[3]AMMON H P, SAFAYHI H, MACK T, et al. Mechanism of antiinflammatory actions of curcumine and boswellic acids[J]. Journal of ethnopharmacology, 1993,38:113-119.
[4]AMMON H,ANAZODO M. Curcumin: A potent inhibitor of leukotriene B4 formation in rat peritoneal polymorphonuclear neutrophils (PMNL) [J]. Planta Medica,1992,58: 226.
[5]CHINARA G. 香菇多糖的免疫药理作用[J]. 国外医药·植物药分册,1993，8(4):165-167.
[6]MARUYAMA S,SUKEKAWA Y,KANEKO Y,et a1. Anti tumor activities of lentinan and micellapist in tumor-bearing mice [J]. Gan To Kagaku Ryoho，2006,33(12)：1726-1729.
[7]张福明，张淑芹，孙非，等. 香菇多糖对单纯疱疹病毒的抑制作用[J]. 长春中医药大学学报，2006，22(4)：11-12.
[8]张涛，柳朝阳，魏凤香，等. 香菇多糖对小鼠免疫功能的影响[J]. 黑龙江医药科学，2003，26(6)：108-109.
[9]赵勇华，于建武，高洁，等. 复方甘草酸苷联合水飞蓟素治疗药物性肝病疗效观察[J]. 肝脏，2007,12（1）:39-40.
[10]燕菲,张巧艳,张宏,等. 五味子与黄芪多糖协同保护对乙酰氨基酚致小鼠急性肝损伤[J]. 药学实践杂志，2009，27（5）：340-343.
[11]倪艳，苏强，刘霞. 黄芪多糖水煎提取工艺的优化试验研究[J]. 中国中药杂志，1998，23(5)：284-286.
[12]DUBOTLS M, GILLES K A ,HAMILTON J K. Colorimetric method for determination of sugars and related substance [J]. Anal Chem, 1956(28): 350-354.
[13]RECKNAGEL R O, GLENDE J R E, DOLAK J, et al. Mechanisms of carbon tetrachloride toxicity [J]. Pharmacology & Therapeutics, 1989, 43 (1): 139-154.
[14]BERRY M N, HALLS H J, GRIVELL M B. Techniques for pharmacological and toxicological studies with isolated hepatocyte suspensions [J]. Life Sciences, 1992,51:1-16.
[15]许治冲，刘晖，徐奇友，等. 温度和饲料脂肪水平对松浦镜鲤免疫及抗氧化能力的影响[J]. 大连海洋大学学报，2012，27(5):429-435.
[16]唐玲，徐奇友，许红，等. 饲料中添加女贞子对镜鲤生长性能、消化酶活性以及肌肉品质的影响[J]. 大连海洋大学学报，2011，26(6):560. 564.
[17]OHTA Y,SASAKI E,NISHIDA K,et al. Contribution of the antilipid peroxidative action of Dai-saiko-to extract to its preventive effect on carbon tetrachloride -induced acute liver injury in rats [J]. Phytotherapy Research,1998，12:5-8.
[18]MURIEL P. NF-κB in liver diseases: a target for drug therapy [J]. J Appl Toxicol, 2009,29:91-100.
[19]REYES-GORDILLO K, SEGOVIA J, SHIBAYAMA M, et al. Curcumin prevents and reverses cirrhosis induced by bile duct obstruction or CCl4 in rats: role of TGF-β modulation and oxidative stress [J]. Fundam Clin Pharmacol, 2008,22:417-427.
[20]ZHANG L J, YU J P, LI D, et al. Effects of cytokines on carbon t etrachloride-induced hepatic fibrogenesis in rats [J]. World J Gastroenterol, 2004,10:77-81.
[21]于岩岩，斯崇文，田秀兰，等. 多种细胞因子单独及协同作用对肝坏死影响的实验研究[J]. 中华医学杂志，1996,76：258-261.
[22]吕邵娃,段继新,郭玉岩,等. 甘草的复方配伍作用机制的研究进展[J]. 中成药，2015,37（9）:2022-2025.
[23]欧小群,黄勤挽,黄攀,等. 姜黄配伍甘草影响姜黄素类化合物提取效果的研究[J]. 中成药，2014,36（10）:2218-2220.
[24]常明向，吴梅梅，李瀚旻. 姜黄素与甘草次酸联用对肝癌HepG-2 细胞增殖的抑制作用[J]. 药物评价研究，2017,40（1）:42-46.
[25]周京辉，魏晓翠，曾志涛. 姜黄素与甘草次酸联合用药的体外抗肿瘤作用[J]. 北方药学，2011,8（4）:7-8.

相似文献/References:

[1]张春云,曹丽萍,杜金梁,等.Nrf2介导姜黄素对四氯化碳诱导鲫鱼肝损伤的保护作用[J].江苏农业学报,2018,(01):114.[doi:doi:10.3969/j.issn.1000-4440.2018.01.017]
　ZHANG Chun-yun,CAO Li-ping,DU Jin-liang,et al.Nrf2 mediate protective effects of curcumin against carbon tetrachloride(CCl4)-induced liver injury in Carassius auratus[J].,2018,(05):114.[doi:doi:10.3969/j.issn.1000-4440.2018.01.017]

备注/Memo

备注/Memo:
收稿日期：2018-06-28 基金项目：中央级公益性科研院所基本科研业务费专项资金项目 (2017JBFZ01)；国家自然科学基金项目（31702318）；江苏自然科学基金项目（BK20170218） 作者简介：曹丽萍(1977-),女,江苏无锡人，博士, 副研究员，研究方向为鱼类免疫和药理学。 (E-mail)caoliping771004@126.com 通讯作者：高崧,（E-mail）gsong@yzu.edu.cn;丁炜东，（E-mail）dingwd@126.com
更新日期/Last Update: 2018-11-05