[1]伍贤军,杨红,盛怡,等.3种蓝细菌藻蓝蛋白β亚基脱辅基蛋白基因的克隆、表达及重组蛋白的抗氧化活性[J].江苏农业学报,2018,(05):998-1004.[doi:doi:10.3969/j.issn.1000-4440.2018.05.005]
 WU Xian-jun,YANG Hong,SHENG Yi,et al.Cloning, expression and antioxidant activities of apo-c-phycocyanin β-subunits from three cyanobacteria species[J].,2018,(05):998-1004.[doi:doi:10.3969/j.issn.1000-4440.2018.05.005]
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3种蓝细菌藻蓝蛋白β亚基脱辅基蛋白基因的克隆、表达及重组蛋白的抗氧化活性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年05期
页码:
998-1004
栏目:
遗传育种·生理生化
出版日期:
2018-10-25

文章信息/Info

Title:
Cloning, expression and antioxidant activities of apo-c-phycocyanin β-subunits from three cyanobacteria species
作者:
伍贤军杨红盛怡冷倩男李萍萍
(南京林业大学生物与环境学院,江苏南京210037)
Author(s):
WU Xian-junYANG HongSHENG YiLENG Qian-nanLI Ping-ping
(College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China)
关键词:
藻蓝蛋白抗氧化活性重组蛋白自由基
Keywords:
phycocyaninantioxidant activityrecombinant proteinfree radical
分类号:
TS202.3
DOI:
doi:10.3969/j.issn.1000-4440.2018.05.005
文献标志码:
A
摘要:
为研究蓝细菌藻蓝蛋白脱辅基蛋白的抗氧化活性,分别从3种蓝细菌Spirulina subsalsa、Mastigocladus laminosus和Nostoc PCC 7120中克隆C-藻蓝蛋白β亚基编码基因cpcBSp、 cpcBMa和cpcBNo,在大肠杆菌中表达,获得了重组蛋白CpcBSp、 CpcBMa和CpcBNo。通过测定其对羟基自由基和DPPH自由基的清除率,比较它们的抗氧化活性。结果显示:3种重组蛋白对羟基自由基和DPPH自由基的清除率都随着蛋白质质量浓度的增加而升高。当蛋白质浓度达到250 mg/L时,CpcBSp、CpcBMa和CpcBNo对羟基自由基的清除率分别为82%、89%和93%,对DPPH自由基的清除率分别为43%、49%和58%。以上结果表明,3种重组藻蓝蛋白脱辅基蛋白都具有较高的抗氧化活性,有潜力作为医疗上使用的抗氧化试剂,不同蓝细菌的同源藻蓝蛋白的抗氧化能力存在一定差异,选择不同蓝细菌的藻蓝蛋白是提高重组藻蓝蛋白抗氧化能力的有效途径。
Abstract:
In order to study the antioxidant activity of apo-phycocyanin, the genes (cpcBSp, cpcBMa and cpcBNo) encoding phycocyanin (C-PC) were cloned from three cyanobacteria species, Spirulina subsalsa, Mastigocladus laminosus and Nostoc PCC 7120. The recombinant proteins, CpcBSp, CpcBMa and CpcBNo, were obtained from Escherichia coli, where the cpcBSp, cpcBMa and cpcBNo genes were expressed respectively. The antioxidant capacities of recombinant CpcBSp, CpcBMa and CpcBNo were evaluated by measuring their hydroxyl radical scavenging activity and DPPH radical scavenging activity. The results showed that the hydroxyl radical and DPPH radical scavenging activity of recombinant proteins increased with the increasing of protein concentration. At a concentration of 250 mg/L, the removal rate of hydroxyl radicals by CpcBSp, CpcBMa and CpcBNo were 82%, 89% and 93%, respectively, whereas CpcBSp, CpcBMa and CpcBNo exhibited DPPH radicals scavenging activity of 43%, 49% and 58%. In conclusion, all three recombinant phycocyanins have high antioxidant activity, and can be used as potent antioxidants in medical treatments. Phycocyanin from different cyanobacteria has some differences in the antioxidant abilities. The choice of C-PC from different algae is an effective way to increase the antioxidant capacities of recombinant phycocyanin.

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备注/Memo

备注/Memo:
收稿日期:2018-03-14 基金项目:中国博士后科学基金项目(14ZR1401500);江苏省自然科学基金青年基金项目(BK20150884);江苏省高等学校大学生创新创业训练计划项目(201610298103X);江苏省高校优势学科建设工程项目(PAPD) 作者简介:伍贤军(1981-),男,湖南新宁人,博士,讲师,主要从事环境生物学研究。(E-mail)xianjunwu2006@163.com
更新日期/Last Update: 2018-11-05