[1]刘军,杨艳,周晓慧,等.应用于茄子的病毒诱导的基因沉默体系的建立与优化[J].江苏农业学报,2018,(04):880-886.[doi:doi:10.3969/j.issn.1000-4440.2018.04.024]
 LIU Jun,YANG Yan,ZHOU Xiao-hui,et al.Establishment and optimization of virus-induced gene silencing in eggplants[J].,2018,(04):880-886.[doi:doi:10.3969/j.issn.1000-4440.2018.04.024]
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应用于茄子的病毒诱导的基因沉默体系的建立与优化()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年04期
页码:
880-886
栏目:
园艺
出版日期:
2018-08-25

文章信息/Info

Title:
Establishment and optimization of virus-induced gene silencing in eggplants
作者:
刘军杨艳周晓慧姜悬云鲍生有庄勇
(江苏省农业科学院蔬菜研究所/江苏省高效园艺作物遗传改良重点实验室,江苏南京210014)
Author(s):
LIU JunYANG YanZHOU Xiao-huiJIANG Xuan-yunBAO Sheng-youZHUANG Yong
(Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory of Horticultural Crop Genetic Improvement, Nanjing 210014,China)
关键词:
茄子VIGS真空侵染萌芽期种子
Keywords:
eggplantvirus-induced gene silencing(VIGS)vacuum-infiltrationgerminated seeds
分类号:
S641.1
DOI:
doi:10.3969/j.issn.1000-4440.2018.04.024
文献标志码:
A
摘要:
本研究以八氢番茄红素脱氢酶(PDS)基因作为指示基因,探讨不同接种方式、培养环境、菌液数量浓度和接种时间对烟草脆裂病毒(TRV)诱导茄子植物内源PDS mRNA沉默效率的影响,进而测试该沉默体系在不同基因型茄子上的沉默效率。结果表明,在昼夜温度25 ℃/20 ℃,16 h光周期条件下, -25 kPa压强下真空抽气2 min渗透侵染露白后 2~3 d的种子能够获得最佳沉默效率;与传统注射压迫法相比真空渗透萌芽种子能够更快、更明显出现沉默表型,缩短试验周期,且该方法在不同基因型茄子中都获得较高沉默效率,说明基于真空侵染萌芽种子法的VIGS体系可应用于茄子基因功能研究。
Abstract:
In this study, phytoene desaturase (PDS) was used as an indicator to explore effects of different inoculation methods, temperatures, microbial concentrations, inoculation time on silencing efficiency of eggplant endogenous PDS mRNA induced by tobacco rattle virus (TRV). Moreover, silencing efficiency of PDS in eggplants with different phenotypes was tested. The results showed that higher silencing efficiency was induced under the conditions of -25 kPa vacuum infiltration for two minutes, the seeds germinated for two to three days, and plant growth temperature at 25 ℃ day and 20 ℃ night. Compared to other techniques such as leaf infiltration, the new inoculation method could obtain phenotype earlier and more obviously and shorten assay period. In addition, the phytoene desaturase gene in different genotypes of eggplants were successfully silenced using the optimized virus-induced gene silencing(VIGS) system, indicating that the VIGS system based on vacuum infiltration of germinated seeds could be used for investigation of gene function in eggplants.

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备注/Memo

备注/Memo:
收稿日期:2017-12-29 基金项目:国家自然科学基金项目(31672145);国家自然科学青年基金项目(31701932);江苏省重点研发计划项目(BE2016329) 作者简介:刘军(1985-),男,硕士,助理研究员,主要从事茄子遗传育种研究。(E-mail)kehl_lau@foxmail.com 通讯作者:庄勇,(E-mail)jaaszy@163.com
更新日期/Last Update: 2018-09-04