[1]沈清清,彭谦,赖泳红,等.光照条件对固定化果胶酶抑藻效应的影响[J].江苏农业学报,2018,(04):842-846.[doi:doi:10.3969/j.issn.1000-4440.2018.04.018]
　SHEN Qing-qing,PENG Qian,LAI Yong-hong,et al.Influence of lighting conditions on the algal-inhibition effect of immobilized pectinase[J].,2018,(04):842-846.[doi:doi:10.3969/j.issn.1000-4440.2018.04.018]
点击复制

光照条件对固定化果胶酶抑藻效应的影响()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年04期
页码:
842-846
栏目:
耕作栽培·资源环境
出版日期:
2018-08-25

文章信息/Info

Title:
Influence of lighting conditions on the algal-inhibition effect of immobilized pectinase
作者:
沈清清1; 彭谦2; 赖泳红2; 纪开燕2
（1.文山学院环境与资源学院，云南文山663099；2.云南大学微生物研究所, 云南昆明650091）
Author(s):
SHEN Qing-qing1; PENG Qian2; LAI Yong-hong2; JI Kai-yan2
（1.College of Environment and Resources，Wenshan University，Wenshan 663099，China；2.Yunnan Institute of Microbiology，Yunnan University，Kunming 650091，China）
关键词:
果胶酶; 铜绿微囊藻; 抑制效应
Keywords:
pectinase; Microcystis aeruginosa; inhibition
分类号:
X52
DOI:
doi:10.3969/j.issn.1000-4440.2018.04.018
文献标志码:
A
摘要:
为探讨固定化果胶酶的抑藻效应机理，测定了光培养和暗培养条件下果胶酶作用后藻细胞的生物量和叶绿素a含量，并在扫描电镜下观察果胶酶对藻细胞内部结构和形态的损伤情况。结果表明，光培养条件下果胶酶抑藻效果显著，试验第30 d时抑藻率达96.31%，叶绿素a含量为0，而暗培养条件下果胶酶对藻细胞生长具有缓慢的促进作用。扫描电镜观察结果显示光照下果胶酶对藻细胞存在不同等级的损伤作用，损伤最严重的藻细胞细胞壁已完全降解，细胞结构完全解体，胞质流出。说明光照条件是果胶酶对铜绿微囊藻起抑制作用的一个敏感条件，光照条件下果胶酶能破坏藻细胞结构、促进叶绿素a降解并抑制藻细胞光合作用，而黑暗条件下果胶酶对藻细胞不表现抑制效应。
Abstract:
To study the inhibition effect and mechanism of immobilized pectinase on Microcystis aeruginosa cells, the biomass of algae cells and the content of chlorophyll a were determined under the conditions of light culture and dark culture after the action of pectinase. The damage of pectinase on the internal structure and morphology of algae cells was observed under scanning electron microscope. The results showed that the algal-inhibition effect of pectinase was remarkable under the condition of light culture. The algal-inhibition rate reached 96.31% on the 30th day of the experiment, and the content of chlorophyll a was 0. In addition, pectinase had slow acceleration on the growth of algae cell under the condition of dark culture. The observed result of scanning electron microscope showed that pectinase had different grades of damage on algae cells under light. The cell wall of the worst damaged algae cells was totally dissolved, and cell structure was completely disintegrated with cytoplasm discharge. In conclusion, light condition is a sensitive condition for pectinase to have inhibiting action on Microcystis aeruginosa. Pectinase can destroy the structure of algae cells, promote the degradation of chlorophyll a and inhibit the photosynthesis of algae cells under light condition. However, pectinase does not show any inhibiting action on algae cells under dark condition.

参考文献/References:

[1]李琦,李国高,刘绍,等. 果胶酶应用的研究进展[J]. 中国农学通报, 2014,30 (21):258-262.
[2]黄俊丽，李常军，王贵学. 微生物果胶酶的分子生物学及其应用研究进展[J]. 生物技术通讯, 2006, 17 (6):992-994.
[3]王海玉,孙珮石,毕晓伊,等. 5株溶藻抑藻细菌的筛选及果胶酶活性的初步研究[J]. 农学学报, 2012, 2(10):62-67.
[4]沈清清，彭谦，赖泳红，等. 固定化果胶酶抑制铜绿微囊藻生长研究[J]. 环境科学, 2012, 33(12): 4316-4321.
[5]刘玉生，韩梅，梁占彬. 光照、温度和营养盐对滇池微囊藻生长的影响[J]. 环境科学研究，1995, 8（6）：7-16.
[6]王蕾,王万贵,蔡禄,等. 果胶酶在制浆中的应用[J]. 天津造纸, 2011 (1):20-23.
[7]FORNI C, TELO F R, CAIOLA M G .Comparative analysis of the polysaccharides produced by different species of Microcystis (Chroococcales, Cyanophyta) [J]. Phycologia, 1997, 36(3):181-185.
[8]郭莉莎,章军,吴娟,等. 黑暗限气条件下铜绿微囊藻细胞死亡的形态结构和生理生化变化[J]. 微生物学报, 2012, 52(2):228-235.
[9]MATSUBARA K, KUBOTA M, ADACHI S, et al. Different mode of cell death induced by calcium ionophore in human Ieukemia cell lines: possible role of constitutive endonuclease[J]. Experimental Cell Research, 1994, 210(1):19-25.
[10]LIU Y S, HAN M, LIANG Z B. Influence of light intensity, temperature and nutrients on the growth of microcystis in water of Dianchi lake[J]. Research of Environmental Sciences, 1995, 8(6):7-11.
[11]SHUNMUGAM S, JOKELA J, WAHLSTEN M, et al. Secondary metabolite from Nostoc XPORK14A inhibits photosynthesis and growth of Synechocystis PCC 6803[J]. Plant, Cell & Environment, 2014, 37(6):1371-1381.
[12]黄思明,尹平河,赵玲. 一株芽孢杆菌对球形棕囊藻的溶藻效果[J]. 暨南大学学报 (自然科学版)，2013，23(3):337-342.
[13]林必桂,杨柳燕,肖琳,等. 赖氨酸对铜绿微囊藻细胞的抑制机理[J]. 生态与农村环境学报,2008,24 (4):68-72.
[14]魏静,王小冬. 南极衣藻对黑暗和低温的适应与恢复[J]. 中国海洋大学学报 (自然科学版), 2013, 43(6):81-86.
[15]MONTECHIARO F, GIORDANO M. Effect of prolonged dark incubation on pigments and photosynthesis of the cave-dwellingCyanobacteria) [J]. Phycologia,2006, 45(6):704-710.

相似文献/References:

[1]李汉全,张炳火,杨建远,等.Streptomyces eurocidicus JXJ 0089对铜绿微囊藻的抑制[J].江苏农业学报,2015,(05):1037.[doi:doi:10.3969/j.issn.1000-4440.2015.05.015]
　LI Han-quan,ZHANG Bing-huo,YANG Jian-yuan,et al.Cyanobacteria-lytic activity of Streptomyces eurocidicus JXJ 0089 against Microcystis aeruginosa[J].,2015,(04):1037.[doi:doi:10.3969/j.issn.1000-4440.2015.05.015]
[2]张海涛,郭西亚,张杰,等.铜绿微囊藻对锌、镉胁迫的生理响应[J].江苏农业学报,2019,(01):33.[doi:doi:10.3969/j.issn.1000-4440.2019.01.005]
　ZHANG Hai-tao,GUO Xi-ya,ZHANG Jie,et al.Physiological response of Microcystis aeruginosa to Zn2+ and Cd2+ stresses[J].,2019,(04):33.[doi:doi:10.3969/j.issn.1000-4440.2019.01.005]

备注/Memo

备注/Memo:
收稿日期：2017-09-22 基金项目：国家自然科学基金项目（30760006）；云南省教育厅项目（2014Y473） 作者简介：沈清清（1980-），女，云南文山人，硕士，副教授，主要从事微生物资源开发与生物信息学应用研究。(E-mail)391291180@qq.com
更新日期/Last Update: 2018-09-04