[1]钟希娜,何涛,葛展霞,等.一种对mcr-1阳性大肠杆菌具有裂解作用的噬菌体生物学特性[J].江苏农业学报,2018,(02):452-458.[doi:doi:10.3969/j.issn.1000-4440.2018.02.033]
　ZHONG Xi-na,HE Tao,GE Zhan-xia,et al.Characterization of a lytic bacteriophage infecting mcr-1-positive Escherichia coli[J].,2018,(02):452-458.[doi:doi:10.3969/j.issn.1000-4440.2018.02.033]
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一种对mcr-1阳性大肠杆菌具有裂解作用的噬菌体生物学特性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年02期
页码:
452-458
栏目:
加工贮藏·质量安全
出版日期:
2018-04-25

文章信息/Info

Title:
Characterization of a lytic bacteriophage infecting mcr-1-positive Escherichia coli
作者:
钟希娜1; 2; 何涛1; 葛展霞1; 王冉1
（1.江苏省农业科学院农业部食品质量安全监控重点开放实验室/江苏省禽产品安全性研究重点实验室，江苏南京210014；2.南京农业大学动物科技学院，江苏南京210095）
Author(s):
ZHONG Xi-na1; 2; HE Tao1; GE Zhan-xia1; WANG Ran1
(1.Key Lab of Food Safety Monitoring and Management, Jiangsu Academy of Agriculture Sciences/Jiangsu Key Laboratory for Animal-derived Food Safety, Ministry of Agriculture, Nanjing 210014,China;2.College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China)
关键词:
mcr-1阳性; 大肠杆菌; 噬菌体; 生物学特性
Keywords:
mcr-1-positive; Escherichia coli; bacteriophage; biological characteristics
分类号:
S852.61+2
DOI:
doi:10.3969/j.issn.1000-4440.2018.02.033
文献标志码:
A
摘要:
本研究以携带多黏菌素耐药基因mcr-1的大肠杆菌SQ-P-E32为宿主菌，分析其对应的裂解性噬菌体的生物学特性。利用双层平板法，从江苏宿迁某猪场污水中分离对该菌具有裂解性的噬菌体，通过透射电镜和基因组酶切分析对噬菌体进行鉴定并命名为vB-EcoP-32，同时测定了该噬菌体的最佳感染复数、一步生长曲线、酸碱和温度耐受性、杀菌效力和裂解谱。结果表明，该噬菌体为短尾噬菌体科，其噬菌斑呈透明的圆形，外周无晕环；该噬菌体的最佳感染复数为100；潜伏期约为5 min，爆发期为55 min，裂解量为32；可耐受温度范围为 30～60 ℃；当pH值为 5～10时其效价稳定；当MOI=100.00时，其对大肠杆菌SQ-P-E32的裂解效果最好；vB-EcoP-32裂解谱较广，对猪源大肠杆菌（包括mcr-1阳性菌株）具有较好的杀灭作用。表明，噬菌体vB-EcoP-32在控制mcr-1阳性大肠杆菌感染方面具有较好的应用前景。
Abstract:
In this study a lytic bacteriophage which could infect Escherichia coli carrying the colistin resistance gene mcr-1 was analyzed for its biological characteristics. The bacteriophage was isolated on host strain E. coli SQ-P-E32 from the sewage of a swine farm in Jiangsu province. By observation of transmission electron microscope and genome digestion analysis, the phage was identified and designated vB-EcoP-32. Meanwhile, the optimal multiplicity of infection (MOI), one-step growth curve, temperature and pH tolerance, lytic effect in vitro of the isolated phage were investigated. Analyzation of the biological characteristics of phage vB-EcoP-32 revealed that it belonged to Podoviridae family, which could form transparent plaques on host strain. The optimal multiplicity of infection (MOI) of the phage was 100. Its latent period was five min while the burst period and burst size were 55 min and 32 PFU/cell, respectively. Phage vB-EcoP-32 was able to survive in a temperature range from 30 ℃ to 60 ℃ and was stable in a pH range from five to ten. Besides, it showed the best efficiency for reducing bacterial growth of E. coli SQ-P-E32 when at the MOI of 100.00. Moreover, the phage showed broad host ranges and had positive effect on E. coli isolates of swine origin(including the mcr-1-positive strains). These results indicated that phage vB-EcoP-32 could be considered as a potential antimicrobial candidate against mcr-1-positive E. coli infection.

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备注/Memo

备注/Memo:
收稿日期：2017-08-17 基金项目：江苏省农业科技自主创新基金项目[CX(16)1060]；江苏省农业科学院基本科研业务专项[ZX(15)6005] 作者简介：钟希娜（1991-），女，山东潍坊人，硕士研究生，主要从事畜产品安全和健康养殖研究。（E-mail）xinazhong@163.com 通讯作者：王冉，（E-mail）wangran2001@126.com
更新日期/Last Update: 2018-05-04