[1]李成,尹莉莉,朱电锋,等.党参多糖对仔猪小肠黏膜免疫功能的影响[J].江苏农业学报,2018,(02):347-355.[doi:doi:10.3969/j.issn.1000-4440.2018.02.018]
　LI Cheng,YIN Li-li,ZHU Dian-feng,et al.Effect of Codonopsis pilosula polysaccharide on small intestinal mucosa immunity of piglets[J].,2018,(02):347-355.[doi:doi:10.3969/j.issn.1000-4440.2018.02.018]
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党参多糖对仔猪小肠黏膜免疫功能的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年02期
页码:
347-355
栏目:
畜牧兽医·水产养殖
出版日期:
2018-04-25

文章信息/Info

Title:
Effect of Codonopsis pilosula polysaccharide on small intestinal mucosa immunity of piglets
作者:
李成; 尹莉莉; 朱电锋; 何嫣婷; 李曼曼; 丁雪东; 李玉; 王希春; 吴金节
（安徽农业大学动物科技学院，安徽合肥230036）
Author(s):
LI Cheng; YIN Li-li; ZHU Dian-feng; HE Yan-ting; LI Man-man; DING Xue-dong; LI Yu; WANG Xi-chun; WU Jin-jie
(College of Animal Science ＆ Technology, Anhui Agricultural University, Hefei 230036，China）
关键词:
党参多糖; 仔猪; 小肠黏膜; 免疫
Keywords:
Codonopsis pilosula polysaccharide; piglet; intestinal mucosa; immune
分类号:
S816.75
DOI:
doi:10.3969/j.issn.1000-4440.2018.02.018
文献标志码:
A
摘要:
为探讨党参多糖（CPP）对仔猪小肠黏膜免疫功能的影响，选取60头1日龄健康“杜×长×大”三元杂交仔猪，随机分为3组，从14 日龄开始，3组仔猪分别饲喂代乳料（对照组）、代乳料＋1%（以生药计）CPP（低剂量组）、代乳料＋2% CPP（高剂量组）。21 日龄断奶，试验期为14 d。试验结束时，每组随机选择6头仔猪进行剖杀，取小肠各段分别放置于4%多聚甲醛和液氮中保存，免疫组化法（IHC）检测分泌型免疫球蛋白A（SIgA）、免疫球蛋白G（IgG）和免疫球蛋白M（IgM）阳性表达水平，逆转录聚合酶链式反应（RT-PCR）测定IgA、IgG和IgM的 mRNA相对表达量。结果表明党参多糖显著或极显著提高仔猪小肠SIgA、IgG和IgM蛋白表达水平以及IgA、IgG和IgM mRNA相对表达量（P<0.01或P<0.05），其中添加2%党参多糖的效果优于添加1%党参多糖的效果。表明在仔猪饲粮中添加党参多糖能够促进仔猪小肠IgA、IgG、IgM mRNA表达，提高仔猪机体免疫应答能力。
Abstract:
This experiment was conducted to analyze the effect of supplementary Codonopsis pilosula polysaccharide (CPP) on small intestinal mucosal immunity of piglets. Sixty crossbred piglets “Duroc × Landrace × Yorkshire” (1-day-old) were randomly divided into three groups. Piglets were fed with milk replacer, milk replacer plus 1%（dry herbs）CPP and milk replacer plus 2% CPP from 14-day-old in control group, low dosage group and high dosage group, respectively. All piglets were weaned at 21 d. The trial was conducted for 14 d. At the end of trial, six piglets from each group were slaughtered. Each segment of the small intestine was collected and placed in 4% paraformaldehyde and liquid nitrogen. The protein positive expression levels of secretory immunoglobulin A (SIgA), immunoglobulin G (IgG) and immunoglobulin M (IgM) were measured by immunohistochemistry (IHC), and the relative expression of IgA, IgG and IgM mRNA were measured by RT-PCR. The results showed that supplementary CPP could elevate significantly or very significantly (P<0.01 or P<0.05) the protein expression levels of SIgA, IgG and IgM and the relative expression of IgA, IgG and IgM mRNA in the small intestine. The adding effect of 2% supplementary CPP was greater than that of 1% supplementary CPP. Supplementary CPP could promote the IgA, IgM, IgG mRNA transcription of small intestine, and enhance immune response in piglets.

参考文献/References:

[1]国家药典委员会.中华人民共和国药典[M].北京:化学工业出版社,2005:199.
[2]王洁,邓长泉,石磊,等.党参的现代研究进展[J].中国医药指南,2011,9(31):279-281.
[3]郭琼琼.党参挥发性成分分析及特殊香气研究[D].太原:山西医科大学,2016.
[4]孙政华,邵晶,郭玫,等.党参化学成分及药理作用研究进展[J].安徽农业科学,2015,43(33): 174-176.
[5]刘合军,印遇龙,孔祥峰,等.中药复方对早期断奶仔猪肠道微生态的影响[J].江苏农业报,2008,24(1):53-58.
[6]张晓君,祝晨,胡黎,等.党参多糖对小鼠免疫和造血功能的影响[J].中药新药与临床药理,2003,14(3):174-176.
[7]徐小波,胡荣,翟永前.中草药添加剂对猪育肥性能和肉质的影响[J].江苏农业学报,2012,28(3):571-574.
[8]石轶男,杨绒娟,扆妍妍,等.党参多糖可溶性粉对肉仔鸡血清ND抗体水平、Ig G及肠道SIgA含量的影响[J].中国兽医杂志,2016,50(9):47-52.
[9]贾宇,王汉,郑晶.复方党参提取物对环磷酰胺处理小鼠免疫功能的调节作用[J].中国实验方剂学杂志, 2011, 17(17): 206-209.
[10]TAN B, LI X G, KONG X F,et al.Dietary L-arginine supplementation enhances the immune status in early-weaned piglets[J].Process in Functional Amino Acids & Carbohydrates for AnimalProduction,2009, 37(2):323-331.
[11]LIN D, SUN S, DU L,et al. Natural and experimental infection of dogs with pandemic H1N1/2009 influenza virus[J].Journal of General Virology,2012,93(1):119.
[12]孙丽华,蒋宗勇,林映才,等.早期断奶仔猪肠黏膜免疫功能研究进展[J].东北农业大学学报,2009,40(1):138-144.
[13]JR HWS, CAVACINI L. Structure and function of immunoglobulins[J].Journal of Allergy & Clinical Immunology,2010,125(2):41-52.
[14]张绍祖.免疫学知识介绍(四)[J].中国实用医刊, 1986(1):36-38.
[15]SEKIROV I, RUSSELL S L, ANTUNES L C M, et al. Gut microbiota in health and disease[J]. Physiological Reviews,2010,90(3):859-904.
[16]李云锋,邓军,张锦华,等.枯草芽孢杆菌对仔猪小肠局部天然免疫及TLR表达的影响[J].畜牧兽医学报,2011,42(4):562-566.
[17]呙于明.动物免疫营养[M].北京:科学出版社, 2011:21-23.
[18]WALKER R I.Considerations for development of whole cell bacterial vaccines to prevent diarrheal diseases in children in developing countries[J].Vaccine,2005,23(26):3369-3385.
[19]PULIMOOD A B, MATHAN M M, MATHAN V I. Quantitative and ultrastructural analysis of rectal mucosal mast cells in acute infectious diarrhea[J].Digestive Diseases & Sciences,1998,43(9):2111-2116.
[20]KEREN D F, BROWN J E, MC DONALD R A, et al. Secretory immunoglobulin A response to Shiga toxin in rabbits:kinetics of the initial mucosal immune response and inhibition of toxicity in vitro and in vivo[J].Infection Immunity,1989,57:1885-1889.
[21]MACPHERSON A J, HUNZIKER L, MCCOY K, et al. IgA responses in the intestinal mucosa against pathogenic and non-pathogenic microorganisms[J].Microbes & Infection, 2001,3(12):1021-1035.
[22]SAINI M S, LIBERATI D M, DIEBEL L N. Sequential changes in mucosal immunity after hemorrhagic shock[J].The American Surgeon,2001,67(8):797-801.
[23]WITTIG B M, ZEITZ M.The gut as an organ of immunology[J].International Journal of Colorectal Disease,2003,18(3):181-187.
[24]杨光,李发胜,刘辉,等.党参多糖对小鼠免疫功能的影响[J].中药药理与临床,2005,21(4):39-39.
[25]刘文生.党参多糖、皂甙提取及对小鼠抗氧化和免疫功能影响的研究[D].晋中:山西农业大学,2004.

备注/Memo

备注/Memo:
收稿日期：2017-08-29 基金项目：科技部科技富民强县专项行动计划项目[国科发农(2012)745号]；安徽省现代农业产业技术体系项目（2016-2020） 作者简介：李成(1992-)，男，安徽阜阳人，硕士研究生，主要从事兽医临床诊疗技术研究。(E-mail)603326759@qq.com。尹莉莉为共同第一作者。 通讯作者：吴金节，(E-mail)wjj@ahau.edu.cn
更新日期/Last Update: 2018-05-04