[1]李学龙,刘国丽,李超,等.蜡样芽胞杆菌菌体及其胞外聚合物对Cd2+的吸附作用[J].江苏农业学报,2018,(02):327-332.[doi:doi:10.3969/j.issn.1000-4440.2018.02.015]
　LI Xue-long,LIU Guo-li,LI Chao,et al.Cd2+ biosorption by Bacillus cereus and its extracellular polymeric substances[J].,2018,(02):327-332.[doi:doi:10.3969/j.issn.1000-4440.2018.02.015]
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蜡样芽胞杆菌菌体及其胞外聚合物对Cd2+的吸附作用()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年02期
页码:
327-332
栏目:
耕作栽培·资源环境
出版日期:
2018-04-25

文章信息/Info

Title:
Cd2+ biosorption by Bacillus cereus and its extracellular polymeric substances
作者:
李学龙; 刘国丽; 李超; 李跃; 杨镇
（辽宁省农业科学院食用菌研究所/辽宁省农业科学院微生物工程中心，辽宁沈阳110161）
Author(s):
LI Xue-long; LIU Guo-li; LI Chao; LI Yue; YANG Zhen
（Institute of Edible Fungi/Research Center of Microbial Engineering, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China）
关键词:
蜡样芽孢杆菌; 胞外聚合物; Cd2+; 生物吸附
Keywords:
Bacillus cereus; extracellular polymeric substances(EPS); Cd2+; biosorption
分类号:
X172
DOI:
doi:10.3969/j.issn.1000-4440.2018.02.015
文献标志码:
A
摘要:
通过液体培养及吸附试验，研究了Cd2+胁迫下蜡样芽胞杆菌生长及其胞外聚合物（EPS）的产生规律、产量和组成，以及菌体和EPS对Cd2+的吸附作用。结果表明，蜡样芽胞杆菌在不同Cd2+浓度胁迫下均可生长，随着Cd2+浓度的增加，各生长时期均有所延后，EPS的生成量与菌体生长曲线具有一定耦合关系，EPS最大生成量为1.707 g/L，多糖与蛋白质含量之和占EPS干质量的 68%～87%。对Cd2+的吸附能力大小顺序为细菌菌体-EPS复合物>EPS>细菌菌体（不含EPS）。透射电镜-X射线光散射能谱（SEM-EDX）分析结果显示，吸附Cd2+后蜡样芽胞杆菌细胞形态及表面结构发生改变，细胞表面形成Cd2+吸收峰，EPS吸附Cd2+后表面形成不规则的含有Cd2+的颗粒状物质。
Abstract:
In this research, the adsorption of Cd2+ by Bacillus cereus and its extracellular polymeric substances (EPS) was studied. The growth of Bacillus cereus and the production rules, yield and composition of its EPS under Cd2+ stress were also investigated through the liquid culture and adsorption experiment. The results showed that Bacillus cereus could grow under different Cd2+ concentrations stress. As the Cd2+ concentration increased, each growth period had been delayed, and the EPS yield had a certain coupling relationship with growth curve of the Bacillus cereus. The maximum yield of EPS was 1.707 g/L. The sugar and protein content accounted for 68 to 87 percent of the dry weight of EPS. The order based on Cd2+ adsorption capacity was as follows: Bacillus cereus> EPS >Bacillus cereus (excluding EPS). The results of SEM-EDX analysis showed that the cell morphology and surface structure of Bacillus cereus had changed after adsorption of Cd2+. The cell surface formed Cd2+ absorption peak after adsorption of Cd2+. EPS surface formed irregular granular material containing Cd2+ after adsorption.

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备注/Memo

备注/Memo:
收稿日期：2017-07-18 基金项目：沈阳市重点科技研发计划项目（17-163-3-00） 作者简介：李学龙(1984-)，男，山东梁山人，硕士，助理研究员，主要从事农田重金属微生物修复技术研究。（E-mail）lixuelong2008@126.com 通讯作者：杨镇，（E-mail）yangzhen@126.com
更新日期/Last Update: 2018-05-04