[1]徐健,李传明,韩光杰,等.颗粒体病毒(CnmeGV)对稻纵卷叶螟的感染及害虫种群增长的影响[J].江苏农业学报,2018,(01):29-33.[doi:doi:10.3969/j.issn.1000-4440.2018.01.004]
 XU Jian,LI Chuan-ming,HAN Guang-jie,et al.The effect of Cnaphalocrocis medinalis granulovirus (CnmeGV) on larva infection and population regulation of rice leaffolder[J].,2018,(01):29-33.[doi:doi:10.3969/j.issn.1000-4440.2018.01.004]
点击复制

颗粒体病毒(CnmeGV)对稻纵卷叶螟的感染及害虫种群增长的影响()
分享到:

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年01期
页码:
29-33
栏目:
植物保护
出版日期:
2018-02-25

文章信息/Info

Title:
The effect of Cnaphalocrocis medinalis granulovirus (CnmeGV) on larva infection and population regulation of rice leaffolder
作者:
徐健1李传明1韩光杰1徐彬1祁建杭2孙俊2刘琴1
(1.江苏里下河地区农业科学研究所,江苏扬州225007;2.扬州绿源生物化工有限公司,江苏扬州225008)
Author(s):
XU Jian1LI Chuan-ming1HAN Guang-jie1XU Bin1QI Jian-hang2SUN Jun2LIU Qin1
(1.Institute of Agricultural Science of the LiXiahe District in Jiangsu Province, Yangzhou 225007, China; 2. Yangzhou Luyuan Bio-chemical Co. Ltd., Yangzhou 225008, China)
关键词:
稻纵卷叶螟颗粒体病毒病毒侵染种群调控
Keywords:
Cnaphalocrocis medinalisgranulovirusvirus infectionpopulation regulation
分类号:
S476+.13
DOI:
doi:10.3969/j.issn.1000-4440.2018.01.004
文献标志码:
A
摘要:
CnmeGV是侵染稻纵卷叶螟的专性杆状病毒。本研究旨在明确田间条件下CnmeGV对稻纵卷叶螟的侵染及种群增长的影响。结果表明,在田间条件下,CnmeGV对稻纵卷叶螟具有较强的致病性,7.500×105OB/m2和1.125×106OB/m2田间喷洒24 d后,害虫感病显症幼虫比例达 69.16%~70.77%。感染病毒幼虫威布尔频数分布拟合存活率曲线表现为死亡率是年龄的增函数,死亡主要发生在幼虫中后期的感病个体中,不同浓度致死中时间为 20.16~21.98 d。种群生命表参数表明CnmeGV对稻纵卷叶螟种群具有明显的干扰控制作用,不同浓度病毒处理区的种群控制指数(IPC)为 0.31~0.32。CnmeGV对稻纵卷叶螟具有致病力强,感病幼虫死亡周期长的特点,田间应用CnmeGV可以显著降低害虫种群增长趋势指数,有效抑制稻纵卷叶螟种群增长。
Abstract:
CnmeGV is a specialized baculovirus and can infect the larvae of rice leaffolder [Cnaphalocrocis medinalis (Guenée)]. The purpose of this paper was to explore the effect of CnmeGV on larvae infection and population regulation of rice leaffolder in rice paddy. CnmeGV had great pathogenicity to the larvae of rice leaffolder in the field. After spraying CnmeGV for 24 days at the concentrations of 7.500×105OB/m2 and 1.125×106OB/m2, larva infective rates ranged from 69.16% to 70.77%. Survival rate curve of infected larva fitted with the model of Weibull frequency was an increasing function of mortality-age, larvae mortality mainly happened in middle and late period. Median lethal time ranged from 20.16 d to 21.98 d at different concentrations.Parameters from life table of natural population indicated that CnmeGV had significant interference effect on the population of rice leaffolder. Index of population control under different concentrations ranged from 0.31 to 0.32. CnmeGV had strong pathogenicity to the rice leaffolder, and the larvae had long death cycle. Field application of CnmeGV reduced the population trend index remarkably and restrained the population growth of rice leaffolder.

参考文献/References:

[1]ROHRMANN G F. Baculovirus molecular biology (3rd edition) [M]. Corvallis: Department of Microbiology Oregon State University, 2014.
[2]POPHAM H J, NUSAWARDANI T, BONNING B C. Introduction to the use of baculoviruses as biological insecticides [J]. Methods in Molecular Biology, 2007,1350(388): 359-366.
[3]SZEWCZYK B, HOYOS-CARVAJAL L, PALUSZEK M, et al. Baculoviruses—re-emerging biopesticides [J]. Biotechnology Advances, 2006, 24(2): 143-160.
[4]秦启联,程清泉,张继红,等.昆虫病毒生物杀虫剂产业化及其展望[J]. 中国生物防治学报, 2012, 28(2): 157-164.
[5]SHEPARD B M, BARRION A T, LISTINGER J A. Rice feeding insects of tropical Asia[M]. Philippines:IRRI,1995.
[6]程家安. 水稻害虫 [M]. 北京: 中国农业出版社,1996.
[7]周奋启,康晓霞,陈银凤,等.扬州市邗江区稻纵卷叶螟发生特点及防治技术[J].江苏农业科学,2016,44(3):140-145.
[8]王琳,包云轩,谢晓金,等.基于地统计法的稻纵卷叶螟时空变化特征[J].江苏农业学报,2017,33(1):50-55.
[9]庞义,濑涌流,刘炬,等.稻纵卷叶螟颗粒体病毒[J]. 微生物学通报,1981, 8(3): 103-104.
[10]张珊,贾茜雯,孙士锋,等.一株稻纵卷叶螟颗粒体病毒的系统发育和流行病学调查 [J]. 环境昆虫学报, 2014,36(5): 756-776.
[11]XU J, LI C M, HAN G J, et al. Cnaphalocrocis medinalis (Guenée) Granulovirus, a potential microbial agent against the rice leaffolder Cnaphalocrocis medinalis Güenée (Lepidoptera: Pyralidae) [J]. Egyptian Journal Biological Pest Control, 2016, 26(4): 675-681.
[12]HAN G J, XU J, LIU Q, et al. Genome of Cnaphalocrocis medinalis granulovirus, the first crambidae-infecting Betabaculovirus isolated from rice leaffolder to sequenced [J]. PLoS ONE, 2016, 11(2): e0147882.
[13]刘琴,徐健,王艳,等. CmGV与Bt对稻纵卷叶螟幼虫的协同作用研究 [J]. 扬州大学学报(农业与自然科学版), 2013,34(4): 89-93.
[14]XU J, LI C M, YANG Y J, et al. Growth and reproduction of artificially fed Cnaphalocrocis medinalis [J]. Rice Science, 2012, 19(3): 247-251.
[15]PINDER J E, WIENER J G, SMITH M H. The weibull distribution: A new method of summarizing survivorship data [J]. Ecology, 1978, 59(5):175-179.
[16]周郁斌,袁中文,李海刚,等. 用Origin软件计算药物溶出度Weibul1分布参数[J].医药导报, 2011, 30(6): 721-723.
[17]SOKAL R R, ROHLF F J. Biometry(3rd edition) [M]. New York: W H Freeman and Company, 1998.
[18]庞雄飞. 害虫种群的生态控制 [M]. 北京: 高等教育出版社, 2002.
[19]LACEY L A, THOMSON D, VINCENT C, et al. Codling moth granulovirus: a comprehensive review [J].BiocontrolScience and Technology, 2008, 18: 639-663.
[20]CORY J S, FRANKLIN M T. Evolution and the microbial control of insects [J]. Evolutionary Applications, 2012, 5(5): 455-469.
[21]HAN G J, LI C M, LIU Q, et al. Synergistic effect of combining Plutella xylostella granulovirus and bacillus thuringiensis at sublethal dosages on controlling of diamondback Moth (Lepidoptera:Plutellidae) [J]. Journal of Economic Entomology, 2015, 108(5):2184-2195.
[22]冼继东,卢传全,庞雄飞. 颗粒体病毒对小菜蛾种群控制作用的评价 [J]. 海南大学学报(自然科学版), 1997, 15(2): 138-140.
[23]FUXA J R. Ecology of insect nucleopolyhedroviruses [J]. Agriculture, Ecosystems and Environment,2004, 103(1): 27-43.
[24]CORY J S, MYERS J. The ecology and evolution of insect baculoviruses [J]. Annual Review of Ecology Evolution and Systematics, 2003, 34(1): 239-272.

相似文献/References:

[1]王琳,包云轩,谢晓金,等.基于地统计法的稻纵卷叶螟时空变化特征[J].江苏农业学报,2017,(01):50.[doi:10.3969/j.issn.1000-4440.2017.01.008 ]
 WANG Lin,BAO Yun-xuan,XIE Xiao-jin,et al.Spatiotemporal characteristics of rice leaf rollers (Cnaphalocrocis medinalis) based on geostatistics method[J].,2017,(01):50.[doi:10.3969/j.issn.1000-4440.2017.01.008 ]
[2]郭铭淇,包云轩,黄璐,等.无人机多光谱影像在稻纵卷叶螟危害监测中的应用[J].江苏农业学报,2023,(07):1530.[doi:doi:10.3969/j.issn.1000-4440.2023.07.010]
 GUO Ming-qi,BAO Yun-xuan,HUANG Lu,et al.Application of multispectral image taken by unmanned aerial vehicle in monitoring Cnaphalocrocis medinalis Güenée damage on rice growth[J].,2023,(01):1530.[doi:doi:10.3969/j.issn.1000-4440.2023.07.010]

备注/Memo

备注/Memo:
收稿日期:2017-07-28 基金项目:江苏省“六大人才高峰”项目(2015-NY-048);江苏省农业三新工程项目[SXGC(2016)224];江苏省农科院颠覆性计划项目[ZX(17)2002];江苏省农业自主创新基金项目[CX(15)1057];扬州市科技支撑计划项目(YZ2015027) 作者简介:徐健(1968-),男,江苏兴化人,博士,研究员,主要从事农业病虫害生物防治研究。(Tel)13901458077;(E-mail)bio-xj@163.com 通讯作者:刘琴,(Tel)0514-87637599;(E-mail)bio-lq@126.com
更新日期/Last Update: 2018-03-06