[1]刘丽,杨静,李成云.玉米-大豆间作对玉米根际氨氧化微生物的影响[J].江苏农业学报,2017,(06):1278-1287.[doi:doi:10.3969/j.issn.1000-4440.2017.06.012]
　LIU Li,YANG Jing,LI Cheng-yun.Effects of maize-soybean intercropping on the ammonia oxidizing microbes in the rhizosphere of maize[J].,2017,(06):1278-1287.[doi:doi:10.3969/j.issn.1000-4440.2017.06.012]
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玉米-大豆间作对玉米根际氨氧化微生物的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年06期
页码:
1278-1287
栏目:
耕作栽培·资源环境
出版日期:
2017-12-30

文章信息/Info

Title:
Effects of maize-soybean intercropping on the ammonia oxidizing microbes in the rhizosphere of maize
作者:
刘丽1; 2; 杨静1; 李成云1
(1.云南生物资源保护与利用国家重点实验室，云南昆明650201；2.西南林业大学生物多样性保护与利用学院，云南省森林灾害预警与控制重点实验室，云南昆明650224)
Author(s):
LIU Li1; 2; YANG Jing1; LI Cheng-yun1
(1. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, China;2.College of Biodiversity Conservation and Utilization, Southwest Forestry University, Key Laboratory of Forest Disater Warning and Control of Yunnan Province, Kunming 650224, China)
关键词:
间作; 玉米根际; 氨氧化微生物; 丰度; 多样性
Keywords:
intercropping; maize rhizosphere; ammonia-oxidation microorganism; abundance; diversity
分类号:
S344.2
DOI:
doi:10.3969/j.issn.1000-4440.2017.06.012
文献标志码:
A
摘要:
以氨单加氧酶基因（amoA）为分子标记，采用real time PCR和克隆文库的方法，研究玉米-大豆间作对玉米根际氨氧化古菌(AOA)和氨氧化细菌(AOB)的丰度、群落多样性及系统发育的影响。结果表明，间作玉米根际总细菌和AOA的数量均显著高于单作玉米根际。概念种或操作分类单元（OTUs）分析结果表明，间作玉米根际AOA和AOB的多样性均高于单作玉米根际。间作玉米根际AOA共获得23个OTU，AOB共获得16个OTU，单作玉米根际AOA和AOB分别获得13个OTU和20个OTU。系统发育分析结果表明，间作抑制了玉米根际AOB的Nitrosomonadaceae类群生长，促进了Nitrosospira cluster 3a类群和1个新类群的生长。AOA则在间作的玉米根际下分散到不同分支中，并在一定程度上促进了cluster water类群和cluster 1类群的生长，抑制了cluster 6类群的生长。
Abstract:
The abundance and composition of maize rhizosphere ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) were investigated by using quantitative real-time polymerase chain reaction, cloning and sequencing approaches based on amoA genes. Intercropping maize rhizosphere had a higher copy numbers of 16 S rRNA and AOA amoA genes than the monoculture maize rhizosphere. Operational taxonomic unit（OTU）analysis results showed that the diversity of AOA and AOB was improved by intercropping. The number of OTU was 23 of AOA and 16 of AOB in intercropping maize rhizosphere. In monoculture maize rhizosphere, the number of OTU was 13 of AOA and 20 of AOB. Phylogenetic analyses of the amoA gene fragments showed that the intercropping inhibited the Nitrosomonadaceae group of AOB and cluster 6 of AOA, but promoted the Nitrosospira cluster 3a and a new lineage of AOB and the cluster water and cluster 1 of AOA.

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备注/Memo

备注/Memo:
收稿日期：2017-06-07 基金项目：国家重点研发计划项目（2017YFD0200400）；云南省林业一级学科项目（No.51600625） 作者简介：刘丽（1976-），女，黑龙江鹤岗人，硕士，高级实验师，主要从事植物相关微生物研究。（Tel）13987126283；（E-mail）cathly@126.com 通讯作者：李成云，（Tel）13708705575；（E-mail）licheng_yun@163.com
更新日期/Last Update: 2018-01-03