[1]高弢,史建荣.基于高通量测序技术分析麝香草酚处理禾谷镰孢菌后转录组学的变化[J].江苏农业学报,2017,(06):1257-1265.[doi:doi:10.3969/j.issn.1000-4440.2017.06.009]
　GAO Tao,SHI Jian-rong.Transcriptome analysis of Fusarium graminearum treated with thymol based on high-throughput sequencing technology[J].,2017,(06):1257-1265.[doi:doi:10.3969/j.issn.1000-4440.2017.06.009]
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基于高通量测序技术分析麝香草酚处理禾谷镰孢菌后转录组学的变化()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年06期
页码:
1257-1265
栏目:
植物保护
出版日期:
2017-12-30

文章信息/Info

Title:
Transcriptome analysis of Fusarium graminearum treated with thymol based on high-throughput sequencing technology
作者:
高弢1; 2; 3; 史建荣 1; 2; 3
(1.江苏省农业科学院农产品质量安全与营养研究所， 江苏南京210014;2.省部共建国家重点实验室培育基地——江苏省食品质量安全重点实验室， 江苏南京210014;3.农业部农产品质量安全控制技术与标准重点实验室， 江苏南京210014)
Author(s):
GAO Tao1; 2; 3; SHI Jian-rong1; 2; 3
(1.Institute of Food Safety and Nutrition， Jiangsu Academy of Agriculture Science， Nanjing 210014， China;2.Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base， Nanjing 210014， China;3.Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality， Ministry of Agriculture， Nanjing 210014， China)
关键词:
禾谷镰孢菌; 麝香草酚; 转录组; 高通量测序
Keywords:
Fusarium graminearum; thymol; transcriptome; high-throughput sequencing technology
分类号:
S435.121.4
DOI:
doi:10.3969/j.issn.1000-4440.2017.06.009
文献标志码:
A
摘要:
植物源化合物麝香草酚能够破坏禾谷镰孢菌细胞膜的完整性来抑制病原菌的生长，但其具体的作用靶标尚不明确。为发掘禾谷镰孢菌中麝香草酚潜在作用靶标，本研究以禾谷镰孢菌标准菌株（PH-1）为材料，采用新一代高通量测序技术 Illumina Hiseq 2000对麝香草酚处理及未处理的禾谷镰孢菌进行转录组测序分析，利用生物信息学方法对差异表达基因的功能进行研究，同时利用实时荧光定量PCR（qRT-PCR）技术对部分关键基因进行验证。结果表明，禾谷镰孢菌经过50 μg/ml麝香草酚处理12 h后，共检测到1 477个基因的表达量发生了变化，其中下调基因数目为772个，上调基因数目为705个。这些差异表达基因参与多种代谢途径，表明麝香草酚对禾谷镰孢菌的作用是一个多基因参与的协同调控过程。qRT-PCR验证部分基因上调下调结果与转录组分析结果一致。本研究通过二代高通量转录组测序技术分析麝香草酚处理后禾谷镰孢菌的差异表达基因，为进一步揭示麝香草酚作用的分子机制，挖掘作用靶标提供基础资料。
Abstract:
Thymol is a kind of phytochemicals, which has antifungal activity against Fusarium graminearum by destroying the integrity of cell membrane. However, the specific targets of thymol are not known. In order to define the potential targets of thymol, a new generation of high-throughput sequencing technology Illumina Hiseq 2000 was used for transcriptome sequencing of F. graminearum treated with thymol, and bioinformatic methods were used for gene function prediction, then the expression of some important genes was monitored by quantitative RT-PCR (qRT-PCR). By transcriptome sequencing, it was found that there were 1 477 differentially expressed genes including 705 up-regulated and 772 down-regulated genes when F. graminearum strain PH-1 was treated with thymol. These differentially expressed genes were involved in multiple metabolic pathways, indicating that the effect of thymol on F. graminearum was a coordination process. Expression of some genes validated by qRT- PCR was consistent with transcriptome analysis. In this study, the second generation of high-throughput transcriptome sequencing technology was used to research the related genes of F. graminearum treated with thymol, which provided theoretical foundation for revealing the molecular mechanism of thymol.

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备注/Memo

备注/Memo:
收稿日期：2017-06-12 基金项目：国家自然科学基金项目(21501692) 作者简介：高弢 (1988-)，女，江苏盐城人，博士，助理研究员，主要从事植物病理学研究。（E-mail）18551694895@163.com 通讯作者：史建荣，(Tel)025-84391613；（E-mail) jianrong63@126.com
更新日期/Last Update: 2018-01-03