[1]齐中强,杜艳,沈乐融,等.转录因子MoOaf22对稻瘟病菌营养生长、细胞壁完整性和胁迫应答的调控[J].江苏农业学报,2017,(06):1242-1248.[doi:doi:10.3969/j.issn.1000-4440.2017.06.007]
 QI Zhong-qiang,DU Yan,SHEN Le-rong,et al.Regulation of transcription factor MoOaf22 in vegetative growth, cell wall integrity and stress response in Magnaporthe oryzae[J].,2017,(06):1242-1248.[doi:doi:10.3969/j.issn.1000-4440.2017.06.007]
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转录因子MoOaf22对稻瘟病菌营养生长、细胞壁完整性和胁迫应答的调控()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年06期
页码:
1242-1248
栏目:
植物保护
出版日期:
2017-12-30

文章信息/Info

Title:
Regulation of transcription factor MoOaf22 in vegetative growth, cell wall integrity and stress response in Magnaporthe oryzae
作者:
齐中强杜艳沈乐融余振仙于俊杰俞咪娜宋天巧张荣胜刘永锋 齐中强杜艳沈乐融余振仙于俊杰俞咪娜宋天巧张荣胜刘永锋 齐中强杜艳沈乐融余振仙于俊杰俞咪娜宋天巧张荣胜刘永锋
(江苏省农业科学院植物保护研究所,江苏南京210014)
Author(s):
QI Zhong-qiangDU YanSHEN Le-rongYU Zhen-xianYU Jun-jieYU Mi-naSONG Tian-qiaoZHANG Rong-shengLIU Yong-feng
(Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
稻瘟病菌转录因子MoOaf22细胞壁完整性胁迫
Keywords:
Magnaporthe oryzaetranscription factor MoOaf22cell wall integritystress
分类号:
S435.111.4+1
DOI:
doi:10.3969/j.issn.1000-4440.2017.06.007
文献标志码:
A
摘要:
为了研究油酸激活型转录因子MoOaf22编码基因MoOAF22(MGG_03413)在稻瘟病菌生长发育及致病过程中的功能,利用基因敲除方法将其进行了缺失突变并对突变体进行了一系列的生物学表型分析。结果表明该基因缺失突变体在完全培养基(CM)上营养生长加快,但在基本培养基(MM)上生长变慢,同时该突变体原生质体释放速度变慢,对荧光增白剂(CFW)和刚果红(Congo Red)耐受性增强,对十二烷基磺酸钠(SDS)、氯化钠(NaCl)和山梨醇(Sorbitol)敏感,但该突变体在产孢量、附着胞形成及致病性等方面较野生型没有明显变化。说明MoOaf22在稻瘟病菌的营养生长、细胞壁完整性和对外界的胁迫应答过程中具有重要的调控作用。
Abstract:
To study the function of oleate-activated transcription factor MoOaf22 coding gene MoOAF22 (MGG_03413) on growth and pathogenicity, the deletion mutant was identified using the knock out method in the rice blast fungus Magnaporthe oryzae. The results indicated that the deletion mutant showed increased growth and reduced growth in complete medium (CM) and minimal medium (MM), respectively. Meanwhile, the rate of protoplast release of the mutant was slower than that of the wild type, and the mutant was more resistant to calcofluor white (CFW) and congo red, in contrast, the mutant was more sensitive to sodium dodecyl sulfate (SDS), sodium chloride (NaCl) and sorbitol. However, the mutant showed no difference in conidiation, appressorium formation and pathogenicity compared with wild type. In summary, MoOaf22 is involved in vegetative growth, cell wall integrity and response to stress of M. oryzae.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2017-05-18 基金项目:国家自然科学基金项目(31401697);江苏省自然科学基金项目(BK20140749);江苏省农业科技自主创新基金项目(CX15-1054) 作者简介:齐中强(1985-),男,河南驻马店人,博士,副研究员,主要从事稻瘟病菌功能基因研究。(Tel)025-84391810;(E-mail)qizhongqiang2006@126.com 通讯作者:刘永锋,(Tel)025-84391002;(E-mail)liuyf@jaas.ac.cn
更新日期/Last Update: 2018-01-03