[1]宁丽华,何晓兰,张大勇.大豆耐盐相关基因GmNcl1功能标记的开发及验证[J].江苏农业学报,2017,(06):1227-1234.[doi:doi:10.3969/j.issn.1000-4440.2017.06.005]
　NING Li-hua,HE Xiao-lan,ZHANG Da-yong.Development and validation of the function marker of soybean salt tolerance gene GmNcl1[J].,2017,(06):1227-1234.[doi:doi:10.3969/j.issn.1000-4440.2017.06.005]
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大豆耐盐相关基因GmNcl1功能标记的开发及验证()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年06期
页码:
1227-1234
栏目:
遗传育种·生理生化
出版日期:
2017-12-30

文章信息/Info

Title:
Development and validation of the function marker of soybean salt tolerance gene GmNcl1
作者:
宁丽华; 何晓兰; 张大勇
（江苏省农业科学院种质资源与生物技术研究所/江苏省农业生物学重点实验室，江苏南京210014）
Author(s):
NING Li-hua; HE Xiao-lan; ZHANG Da-yong
(Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences/ Provincial Key Laboratory of Agrobiology, Nanjing 210014, China)
关键词:
大豆; 耐盐性; GmNcl1; 酶切扩增多态性序列（CAPS）标记
Keywords:
soybean; salt tolerance; GmNcl1; cleaved amplified polymorphic sequences(CAPS) mark
分类号:
S565.1
DOI:
doi:10.3969/j.issn.1000-4440.2017.06.005
文献标志码:
A
摘要:
土壤盐渍化严重影响大豆生产，因而鉴定大豆耐盐种质的分子标记对大豆耐盐新品种的培育具有重要意义。本研究分析了大豆耐盐相关基因GmNcl1等位变异位点的限制性酶切位点，通过酶切PCR产物，琼脂糖凝胶电泳和酶切片段分析，开发建立了分子标记CAPS/Xba I。利用该标记对10份不同耐盐大豆种质进行酶切分型鉴定，然后通过测序试验进行验证。结果表明，用所开发的共显性标记CAPS/Xba I对10份大豆种质进行耐盐性鉴定，鉴定结果与依据表型进行鉴定的结果一致。由此可见，CAPS/Xba I可用于大豆品种的耐盐性鉴定。
Abstract:
Soil salinization will lead to serious reduction of yield and affect the quality of soybean. Therefore, identifying molecular markers linked to the function gene of salt tolerance will be helpful to soybean breeding. On the basis of the allelic variations of GmNcl1, which was a salt tolerance gene, a pair of primers was designed, and the method of restriction analysis was used to develop function marker. Finally, a cleaved amplified polymorphic sequences(CAPS) marker named CAPS/Xba I was developed. Ten different salt tolerance characteristic soybean cultivars have been screened by this marker. The result of salt tolerance soybean identified by the codominant marker was consisted with the result of that identified according to phenotype. So, CAPS/Xba I could be used in the identification of soybean salt tolerance.

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备注/Memo

备注/Memo:
收稿日期：2017-08-31 基金项目：国家自然科学基金项目（31701453）；江苏省自然科学基金项目（BK20160584） 作者简介：宁丽华（1985-），女，山东泰安人，博士，助理研究员，主要从事农学研究。(Tel) 025-84391957；(E-mail)NLH_2015@126.com 通讯作者：张大勇，(E-mail)cotton.z@j126.com
更新日期/Last Update: 2018-01-03