[1]李素梅,施卫明.RNAi沉默水稻铵转运体OsAMT1表达的生长效应[J].江苏农业学报,2017,(06):1201-1206.[doi:doi:10.3969/j.issn.1000-4440.2017.06.001]
 LI Su-mei,SHI Wei-ming.The growth effect of rice by RNAi silencing ammonium transporter OsAMT1[J].,2017,(06):1201-1206.[doi:doi:10.3969/j.issn.1000-4440.2017.06.001]
点击复制

RNAi沉默水稻铵转运体OsAMT1表达的生长效应()
分享到:

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年06期
页码:
1201-1206
栏目:
遗传育种·生理生化
出版日期:
2017-12-30

文章信息/Info

Title:
The growth effect of rice by RNAi silencing ammonium transporter OsAMT1
作者:
李素梅1施卫明2
(1.南京理工大学化工学院,江苏 南京210094;2.土壤与农业可持续发展国家重点实验室,中国科学院南京土壤研究所,江苏 南京 210008)
Author(s):
LI Su-mei1SHI Wei-ming2
(1.School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing, 210094, China;2.State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China)
关键词:
OsAMT1RNAi表达水平生物量氮累积量
Keywords:
OsAMT1RNAiexpression levelbiomassnitrogen accumulation
分类号:
Q786;Q945.12
DOI:
doi:10.3969/j.issn.1000-4440.2017.06.001
文献标志码:
A
摘要:
OsAMT1是水稻中负责铵态氮高亲和吸收的基因家族,包括OsAMT1;1~OsAMT1;3 3个基因,已有的研究对其基因表达特征以及调控进行了大量的分析。OsAMT1;1表达水平最高,OsAMT1;2受氮水平诱导最为显著。因此,本研究选取OsAMT1序列上高度同源的区域,利用反向遗传学的RNAi技术构建再生OsAMT1家族3个基因共干扰沉默的转基因植株,分析OsAMT1基因表达水平降低引起的植株生长和氮累积量的变化。结果表明,依据OsAMT1同源序列构建的转基因水稻幼苗在低氮条件下基因的表达水平下降了 28%~60%,幼苗生长受到明显的抑制,氮累积量也受到显著抑制,说明OsAMT1在水稻的高亲和吸收铵态氮中占有重要地位。
Abstract:
OsAMT1 is a gene family responsible for high affinity ammonium uptake of rice, including OsAMT1;1, OsAMT1;2 and OsAMT1;3, which has been extensively studied for its function, expression and regulation. OsAMT1;1 was the biggest abundance gene in OsAMT1 family and OsAMT1; 2 was the most significant induction gene to nitrogen starvation. Therefore, in this study, we selected the homology sequence of OsAMT1 to construct the co-interfered transgenic plants through reverse genetics RNAi technology, subsequently analyzed the growth of transgenic plants caused by gene silencing. The results showed that the expression level of transgenic rice seedlings was decreased by 28%-60% under low nitrogen condition, the growth of seedlings was inhibited by OsAMT1 co-interfering, and the nitrogen accumulation was significantly inhibited, which indicated that OsAMT1 played an important role in rice high-affinity ammonium uptake.

参考文献/References:

[1]SONODA Y, IKEDA A, SAIKI S, et al. Distinct expression and function of three ammonium transporter genes (OsAMT1;1-1;3) in rice[J].Plant and Cell Physiology, 2003, 44: 726-734.
[2]YANG S, HAO D, CONG Y, et al. The rice OsAMT1;1 is a proton-independent feedback regulated ammonium transporter[J]. Plant Cell Rep, 2015, 34(2):321-330.
[3]LOQU D, YUAN L, KOJIMA S, et al. Additive contribution of AMT1;1 and AMT1;3 to high-affinity ammonium uptake across the plasma membrane of nitrogen-deficient Arabidopsis roots[J]. The Plant Journal, 2006, 48:522-534.
[4]PASTOR V, GAMIR J, CAMANES G, et al. Disruption of the ammonium transporter AMT1.1 alters basal defenses generating resistance against Pseudomonas syringae and Plectosphaerella cucumerina[J]. Frontiers in Plant Science, 2014, 5: 231-247.
[5]LI S M, LI B Z, SHI W M. Expression patterns of nine ammonium transporters in rice in response to N status[J]. Pedosphere, 2012, 22(6): 860-869.
[6]赵首萍,赵学强,施卫明.不同铵硝比例对水稻铵吸收代谢基因表达的影响[J].土壤学报,2006,43(3):436-442.
[7]曹玉,李素梅,施卫明,等.水稻吸铵基因OsAMT1;2和OsAMT3;3在不同生育期中的表达量差异及其在酵母细胞中吸铵功能初析[J].土壤,2009,41(4):612-619.
[8]RANATHUNGE K, EL-KEREAMY A, GIDDA S, et al. AMT1;1 transgenic rice plants with enhanced NH4(+) permeability show superior growth and higher yield under optimal and suboptimal NH4(+) conditions[J]. Journal of Experimental Botany, 2014, 65(4):965-979.
[9]杨玛丽,赵统敏,余文贵,等.转基因RNAi技术在番茄研究中的应用[J].江苏农业学报,2015,31(1):217-221.
[10]WANG F, LI W, ZHU J, et al. Hairpin RNA targeting multiple viral genes confers strong resistance to rice black-streaked dwarf virus[J]. International Journal of Moleculor Science, 2016 , 17 (5) :705.
[11]KATOCH R, THAKUR N. Advances in RNA interference technology and its impact on nutritional improvement, disease and insect control in plants[J]. Applied Biochemistry Biotechnology, 2013, 169(5): 1579-1605.
[12]周雅智,姜玲,黄勇. BraSDG2 RNA干扰载体的构建和拟南芥的遗传转化[J].山东农业科学,2016,48(10):7-11.
[13]弓雪,姜敏,齐欣,等.玉米ZmSUT4基因 RNAi 植物表达载体的构建[J].江苏农业科学,2016,44(7):42-44.
[14]MIKI D, ITOH R, SHIMAMOTO K. RNA silencing of single and multiple members in a gene family of rice[J]. Plant Physiology, 2005,138(4):1903-1913.
[15]KAISER B N, RAWAT S R, SIDDIQI M Y, et. Functional analysis of an Arabidopsis T-DNA ‘knockout’ of the high-affinity NH+4 transporter AtAMT1;1[J]. Plant Physiology, 2002, 130:1263-1275.
[16]YUAN L, LOQU D, KOJIMA S, et al. The organization of high-affinity ammonium uptake in Arabidopsis roots depends on the spatial arrangement and biochemical properties of AMT1-type transporters[J]. Plant Cell, 2007, 19(8):2636-2652.
[17]HOQUE M S, MASLE J, UDVARDI M K, et al. Over-expression of the rice OsAMT1-1 gene increases ammonium uptake and content, but impairs growth and development of plants under high ammonium nutrition[J]. Functional Plant Biology, 2006, 33:153-163.
[18]BAO A, LIANG Z, ZHAO Z, et al. Overexpressing of OsAMT1-3, a high affinity ammonium transporter gene, modifies rice growth and carbon-nitrogen metabolic status[J]. International Journal of Molecular Sciences,2015, 16: 9037-9063.

相似文献/References:

[1]杨玛丽,赵统敏,余文贵,等.转基因 RNAi 技术在番茄研究中的应用[J].江苏农业学报,2015,(01):217.[doi:10.3969/j.issn.1000-4440.2015.01.034]
 YANG Ma-li,ZHAO Tong-min,YU Wen-gui,et al.Progress in RNA interference technique applied in tomato[J].,2015,(06):217.[doi:10.3969/j.issn.1000-4440.2015.01.034]
[2]曲绍轩,骆昕,徐平,等.腐食酪螨对阿维菌素的抗性相关基因鉴定与功能分析[J].江苏农业学报,2022,38(03):617.[doi:doi:10.3969/j.issn.1000-4440.2022.03.006]
 QU Shao-xuan,LUO Xin,XU Ping,et al.Identification and functional analysis of resistance genes of Tyrophagus putrescentiae Schrank to abamectin[J].,2022,38(06):617.[doi:doi:10.3969/j.issn.1000-4440.2022.03.006]

备注/Memo

备注/Memo:
收稿日期:2017-05-22 基金项目:国家自然科学青年基金项目(30800702) 作者简介:李素梅(1978-),女,安徽合肥人,博士,研究方向为氮素吸收转运机制。(E-mail)smli321@163.com
更新日期/Last Update: 2018-01-03