[1]马欣雨,宋雅萍,焦若普,等.牛bta-miR-15a靶基因的预测及其在脂肪细胞分化过程中的表达模式鉴定[J].江苏农业学报,2026,42(06):1202-1210.[doi:doi:10.3969/j.issn.1000-4440.2026.06.012]
　MA Xinyu,SONG Yaping,JIAO Ruopu,et al.Prediction of bovine bta-miR-15a target gene and identification of its expression pattern during adipocyte differentiation[J].,2026,42(06):1202-1210.[doi:doi:10.3969/j.issn.1000-4440.2026.06.012]
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牛bta-miR-15a靶基因的预测及其在脂肪细胞分化过程中的表达模式鉴定()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年06期
页码:
1202-1210
栏目:
畜牧兽医·水产养殖
出版日期:
2026-06-30

文章信息/Info

Title:
Prediction of bovine bta-miR-15a target gene and identification of its expression pattern during adipocyte differentiation
作者:
马欣雨1; 2; 宋雅萍1; 2; 焦若普1; 2; 马海楠1; 2; 姜超1; 2; 宋小雨1; 2; 杨东梅1; 2; 龚红芳1; 2; 马艺伦1; 2; 魏大为1; 2
(1.宁夏大学动物科技学院,宁夏银川750021；2.宁夏反刍动物分子细胞育种重点实验室,宁夏银川750021)
Author(s):
MA Xinyu1; 2; SONG Yaping1; 2; JIAO Ruopu1; 2; MA Hainan1; 2; JIANG Chao1; 2; SONG Xiaoyu1; 2; YANG Dongmei1; 2; GONG Hongfang1; 2; MA Yilun1; 2; WEI Dawei1; 2
(1.School of Animal Science and Technology, Ningxia University, Yinchuan 750021, China;2.Key Laboratory of Ruminant Molecular Cell Breeding, Ningxia Hui Autonomous Region, Yinchuan 750021, China)
关键词:
牛bta-miR-15a; 生物信息学分析; 脂肪细胞分化; 表达模式
Keywords:
bovine bta-miR-15a; bioinformatics analysis; adipocyte differentiation; expression pattern
分类号:
S823.2
DOI:
doi:10.3969/j.issn.1000-4440.2026.06.012
文献标志码:
A
摘要:
为了探究bta-miR-15a的生物学特性，鉴定其在脂肪分化过程中的表达模式,应用生物信息学分析方法,研究miR-15a在不同物种中的保守性,预测其潜在靶基因,并对其进行功能富集分析。应用实时荧光定量PCR(Quantitative real-time polymerase chain reaction,RT-qPCR)技术鉴定miR-15a在牛背最长肌、皮下脂肪中的相对表达量,探究其在肌内前体脂肪细胞不同分化阶段的表达模式。结果表明,miR-15a在不同物种间具有高度保守性；借助TargetScan、miRWalk软件共预测出7 582个候选靶基因,整合后得到462个候选的共有靶基因；靶基因主要在对生长因子的应答、细胞分化的负调控及Wnt信号通路等基因本体(GO)条目上富集。基于京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)数据库的信号通路富集分析结果显示,其靶基因主要富集在Notch、丝裂原活化蛋白质激酶(MAPK)、鞘脂等信号通路中。进一步通过RT-qPCR分析发现,bta-miR-15a在安格斯牛、西门塔尔牛皮下脂肪中的相对表达量极显著高于背最长肌中的相对表达量(P<0.01),并且其在西门塔尔牛皮下脂肪中的相对表达量显著高于安格斯牛皮下脂肪中的相对表达量(P<0.05),在安格斯牛中，成年牛背最长肌组织中的相对表达量极显著高于犊牛背最长肌组织(P<0.01)。研究还发现,bta-miR-15a在牛肌内脂肪分化阶段的时序表达过程中呈现先上升后下降的变化。上述结果表明,bta-miR-15a在牛脂肪的沉积过程中扮演重要角色,为探究其在脂肪细胞成脂分化过程中的调控作用奠定了基础。
Abstract:
To investigate the biological characteristics of bta-miR-15a and identify its expression pattern during adipogenic differentiation, bioinformatics analysis was employed to examine the conservation of miR-15a across different species, predict its potential target genes, and perform functional enrichment analysis. Quantitative real-time polymerase chain reaction (RT-qPCR) was applied to determine the relative expression of miR-15a in bovine longissimus dorsi muscle and subcutaneous adipose tissue, and to investigate its expression patterns at different stages of intramuscular preadipocyte differentiation. The results showed that miR-15a was highly conserved across different species. A total of 7 582 candidate target genes were predicted using TargetScan and miRWalk software, and 462 common candidate target genes were obtained after integration. The target genes were mainly enriched in Gene Ontology (GO) terms including response to growth factors, negative regulation of cell differentiation, and Wnt signaling pathway. Pathway enrichment analysis based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) database revealed that the target genes were mainly enriched in Notch, mitogen-activated protein kinase (MAPK), and sphingolipid signaling pathways. Further RT-qPCR analysis revealed that the relative expression of bta-miR-15a was significantly higher in subcutaneous adipose tissue than in longissimus dorsi muscle in both Angus and Simmental cattle (P<0.01), and its expression was significantly higher in subcutaneous adipose tissue of Simmental cattle compared with that of Angus cattle (P<0.05), while the relative expression in longissimus dorsi muscle of adult Angus cattle was significantly higher than that of Angus calves (P<0.01). Additionally, the temporal expression of bta-miR-15a during bovine intramuscular adipocyte differentiation exhibited a pattern of first increasing and then decreasing. These results indicate that bta-miR-15a plays an important role in bovine fat deposition, laying a foundation for further investigation into its regulatory mechanism during adipogenic differentiation.

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备注/Memo

备注/Memo:
收稿日期：2025-06-11基金项目：宁夏重点研发计划项目(2023BCF01006、2024BBF01007)；宁夏自然科学基金项目(2025AAC040003、2025AAC030558)；宁夏高等教育本科人才培养提升计划项目作者简介：马欣雨(2002-),女,宁夏吴忠人,本科生,研究方向为牛的重要经济性状。(E-mail)13995035323@163.com通讯作者：魏大为,(E-mail)weidawei@nxu.edu.cn
更新日期/Last Update: 2026-07-15