[1]侯超文,王志辉,李晓玲,等.基于农艺性状和SSR分子标记的百合遗传多样性及关联分析[J].江苏农业学报,2026,42(06):1135-1147.[doi:doi:10.3969/j.issn.1000-4440.2026.06.006]
　HOU Chaowen,WANG Zhihui,LI Xiaoling,et al.Genetic diversity and association analysis of lily based on agronomic traits and SSR molecular markers[J].,2026,42(06):1135-1147.[doi:doi:10.3969/j.issn.1000-4440.2026.06.006]
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基于农艺性状和SSR分子标记的百合遗传多样性及关联分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年06期
页码:
1135-1147
栏目:
遗传育种·生理生化
出版日期:
2026-06-30

文章信息/Info

Title:
Genetic diversity and association analysis of lily based on agronomic traits and SSR molecular markers
作者:
侯超文; 王志辉; 李晓玲; 龚秀娟; 刘泽湘; 蒋林芳; 杨康怡; 姜晓; 童巧珍
(湖南中医药大学药学院/湘产大宗道地药材种质资源及规范化种植重点研究室/湖南省普通高等学校中药现代化研究重点实验室,湖南长沙410208)
Author(s):
HOU Chaowen; WANG Zhihui; LI Xiaoling; GONG Xiujuan; LIU Zexiang; JIANG Linfang; YANG Kangyi; JIANG Xiao; TONG Qiaozhen
(School of Pharmacy, Hunan University of Chinese Medicine/Key Laboratory for Germplasm Resources and Standardized Planting of Genuine Medicinal Materials Produced in Hunan Province/Key Laboratory of Modern Research of Traditional Chinese Medicine, Education Department of Hunan Province, Changsha 410208, China)
关键词:
百合; 简单序列重复(SSR)分子标记; 农艺性状; 遗传多样性; 关联分析
Keywords:
lily; simple sequence repeat (SSR) molecular markers; agronomic traits; genetic diversity; association analysis
分类号:
S644.1
DOI:
doi:10.3969/j.issn.1000-4440.2026.06.006
文献标志码:
A
摘要:
为深入了解百合种质资源的遗传背景,挖掘与农艺性状相关联的分子标记,本研究以20个百合种群的200份百合样品为研究对象,分析其14个农艺性状和简单序列重复(SSR)分子标记的遗传多样性,并采用t检验进行二者的关联性分析。结果表明,14个农艺性状变异系数介于19.48%～87.23%,均值为41.89%,表现出丰富的表型遗传多样性；20个百合种群的观测等位基因数、有效等位基因数、观测杂合度、期望杂合度及香农指数分别为1.706～1.882、1.612～1.859、0.447～0.605、0.594～0.835和0.317～0.435,种群间遗传分化系数为0.579 6,基因流为0.362 7,种群间变异占比(56%)高于种群内(44%),遗传距离与相似度均值分别为0.070 4和0.932 4,表明种群间存在显著遗传分化且基因交流较少。聚类分析结果与Structure分析结果相互验证,可有效区分龙牙百合、金百合、卷丹3个百合类型,并将同一种群的10个样品聚为一类,但部分地理位置相近的种群未聚为一类。关联分析筛选出7个SSR分子标记(SSR-16、SSR-40、SSR-59、SSR-71、SSR-77、SSR-84、SSR-87)均与株高、茎粗等5个农艺性状显著关联。综上,供试20个百合种群均具有较高遗传丰富度,筛选出的SSR分子标记可为百合遗传改良提供重要参考依据。
Abstract:
To gain a deeper understanding of the genetic background of lily germplasm resources and to identify molecular markers associated with agronomic traits, this study analyzed 200 lily samples from 20 lily populations. The genetic diversity of 14 agronomic traits and simple sequence repeat (SSR) molecular markers was assessed, and the association between them was examined using the t-test. The results showed that the coefficients of variation for the 14 agronomic traits ranged from 19.48% to 87.23%, with a mean of 41.89%, indicating abundant phenotypic genetic diversity. For the 20 lily populations, the observed number of alleles, effective number of alleles, observed heterozygosity, expected heterozygosity, and Shannon’s information index were 1.706-1.882, 1.612-1.859, 0.447-0.605, 0.594-0.835, and 0.317-0.435, respectively. The genetic differentiation coefficient of 20 lily populations was 0.579 6, and the gene flow was 0.362 7. The proportion of variation among populations (56%) was higher than that within populations (44%). The mean genetic distance and genetic similarity were 0.070 4 and 0.932 4, respectively, indicating significant genetic differentiation among populations and limited gene flow. The results of cluster analysis and Structure analysis validated each other. The three lily types, namely Longya lily, Jin lily, and Juandan (Lilium lancifolium), could be effectively distinguished, and the ten samples from the same population were clustered into one group. However, some populations with close geographical locations were not clustered together. Seven SSR markers (SSR-16, SSR-40, SSR-59, SSR-71, SSR-77, SSR-84, and SSR-87) were identified by association analysis, and all were significantly associated with five agronomic traits, including plant height and stem diameter. In conclusion, all 20 tested lily populations exhibited high genetic richness, and the identified SSR markers can provide important references for genetic improvement of lily.

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备注/Memo

备注/Memo:
收稿日期：2025-08-14基金项目：国家中药材产业技术体系食用百合龙山综合试验站资助项目(CARS-21)；龙山百合生态种植及品质提升关键技术研究与示范项目(2023NCKJTPY0276)作者简介：侯超文(1998-),男,山东烟台人,硕士研究生,研究方向为中药资源与开发。(E-mail)2813487381@qq.com通讯作者：童巧珍,(E-mail)410849649@qq.com
更新日期/Last Update: 2026-07-15