[1]李雨欣,李静,赵洁,等.水稻低温发芽和穗发芽特性相关分子标记qLTG3-1的开发与应用[J].江苏农业学报,2026,42(06):1125-1134.[doi:doi:10.3969/j.issn.1000-4440.2026.06.005]
　LI Yuxin,LI Jing,ZHAO Jie,et al.Development and application of the molecular marker qLTG3-1 associated with low-temperature germination and pre-harvest sprouting traits in rice[J].,2026,42(06):1125-1134.[doi:doi:10.3969/j.issn.1000-4440.2026.06.005]
点击复制

水稻低温发芽和穗发芽特性相关分子标记qLTG3-1的开发与应用()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年06期
页码:
1125-1134
栏目:
遗传育种·生理生化
出版日期:
2026-06-30

文章信息/Info

Title:
Development and application of the molecular marker qLTG3-1 associated with low-temperature germination and pre-harvest sprouting traits in rice
作者:
李雨欣1; 2; 李静2; 3; 赵洁2; 3; 黄奕2; 3; 吴莹莹2; 4; 王芳权2; 5; 李霞1; 2; 3; 5; 杨杰2; 5; LPEZ LZARO A Maqueira6
(1.南京农业大学生命科学学院,江苏南京210095;2.江苏省农业科学院粮食作物研究所/生物育种钟山实验室/农业农村部淮河下游种质创新重点实验室,江苏南京210014;3.南京林业大学生命科学院,江苏南京210037；4.江苏大学生物科学与技术学院,江苏镇江212013；5.江苏省粮食作物现代产业技术协同创新中心,江苏扬州225009;6.古巴国家农业科学研究所,圣何塞·德拉拉斯CG32700)
Author(s):
LI Yuxin1; 2; LI Jing2; 3; ZHAO Jie2; 3; HUANG Yi2; 3; WU Yingying2; 4; WANG Fangquan2; 5; LI Xia1; 2; 3; 5; YANG Jie2; 5; LPEZ LZARO A Maqueira6
(1.College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China;2.Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Zhongshan Biological Breeding Laboratory/Key Laboratory of Germplasm Innovation in Downstream of Huaihe River (Nanjing), Ministry of Agriculture and Rural Affairs, Nanjing 210014, China;3.College of Life Sciences, Nanjing Forestry University, Nanjing 210037, China；4.School of Biological Science and Technology, Jiangsu University, Zhenjiang 212013, China;5.Collaborative Innovation Center for Modern Production Technology of Grain Crops of Jiangsu Province, Yangzhou 225009, China;6.Instituto Nacional de Ciencias Agrícolas (INCA), San José de las Lajas CG32700, Cuba)
关键词:
水稻; 低温发芽; 穗发芽; qLTG3-1
Keywords:
rice; low-temperature germination; pre-harvest sprouting; qLTG3-1
分类号:
S511.032
DOI:
doi:10.3969/j.issn.1000-4440.2026.06.005
文献标志码:
A
摘要:
为了筛选水稻(Oryza sativa L.)对低温发芽(Low temperature germination,LTG)和穗发芽(Pre-harvest sprouting, PHS)的抗性,本研究对29份水稻品种(品系)的低温发芽特性和穗发芽特性表型进行分析,并用K-均值聚类法对各水稻品种(品系)进行分类；基于水稻低温发芽相关主效基因qLTG3-1编码区的71 bp缺失变异,开发特异性分子标记,通过表型-基因型关联分析验证标记的准确性；应用开发的特异性分子标记对646份水稻品种(品系)[含557份亲本品种(品系)和89份江苏迟熟中粳品种(品系)]进行抗性筛选。聚类分析结果显示,48%的水稻品种(品系)属于耐LTG、易PHS型(聚类1),52%的水稻品种(品系)属于不耐LTG、耐PHS型(聚类2)。经表型-基因型关联验证,发现标记qLTG3-1的区分准确率达75%～86%。646份水稻品种(品系)的筛选结果显示,85.5%的水稻品种(品系)为耐LTG、易PHS型,14.5%的水稻品种(品系)为不耐LTG、耐PHS型。本研究开发的分子标记可同步筛选水稻的LTG、PHS特性,从而为抗性水稻品种(品系)的选育提供技术参考。
Abstract:
To screen rice (Oryza sativa L.) for resistance to low-temperature germination (LTG) and pre-harvest sprouting (PHS), this study analyzed the phenotypic characteristics of LTG and PHS in 29 rice varieties (lines), and classified these varieties (lines) using K-means clustering analysis. Based on a 71 bp deletion in the coding region of the major LTG-related gene qLTG3-1, a specific molecular marker was developed, and the accuracy of the marker was validated through phenotype-genotype association analysis. The developed specific molecular marker was applied to screen 646 rice varieties (lines), including 557 parental varieties (lines) and 89 late-maturing medium japonica varieties (lines) from Jiangsu province, for resistance. The clustering analysis results showed that 48% of the rice varieties (lines) belonged to the LTG-tolerant/PHS-susceptible type (Cluster 1), and 52% belonged to the LTG-susceptible/PHS-tolerant type (Cluster 2). After phenotype-genotype association validation, it was found that the discrimination accuracy of marker qLTG3-1 reached 75%-86%. The screening results of 646 rice varieties (lines) showed that 85.5% were LTG-tolerant/PHS-susceptible, and 14.5% were LTG-susceptible/PHS-tolerant. The molecular marker developed in this study can simultaneously screen for LTG and PHS characteristics in rice, thus providing a technical reference for breeding resistant rice varieties (lines).

参考文献/References:

[1]FUJINO K, SEKIGUCHI H. Origins of functional nucleotide polymorphisms in a major quantitative trait locus,qLTG3-1,controlling low-temperature germinability in rice[J]. Plant Molecular Biology,2011,75(1/2):1-10.
[2]周旭,杨梯丰,刘祖培,等. 水稻低温发芽力的研究进展与展望[J]. 中国稻米,2024,30(5):41-48.
[3]余木兰,唐宁,夏玉梅,等. 水稻穗萌机制及调控的研究进展[J]. 杂交水稻,2020,35(5):1-6.
[4]卢心明,牛百晓. 水稻种子休眠遗传调控机制研究进展[J]. 扬州大学学报(农业与生命科学版),2024,45(1):1-9.
[5]XU F, TANG J Y, GAO S P, et al. Control of rice pre-harvest sprouting by glutaredoxin-mediated abscisic acid signaling[J]. The Plant Journal:for Cell and Molecular Biology,2019,100(5):1036-1051.
[6]周述波,林伟,贺立静,等. 水稻穗上芽发生的生理变化初步研究[J]. 杂交水稻,2011,26(4):68-71.
[7]赵庆勇,陈涛,赵春芳,等. 粳稻穗发芽的品种(系)间差异及其影响因素分析[J]. 江苏农业学报,2017,33(3):481-486.
[8]裘实,卫平洋,魏海燕,等. 穗发芽程度对粳稻稻米品质和蛋白质组分的影响[J]. 江苏农业学报,2019,35(3):523-530.
[9]SASAKI T, KINOSHITA T, TAKAHASHI M E. Estimation of the numbar of genes in the germination ability at low temperature in rice:genetical studies in rice plant,LVII[J]. Journal of the Faculty of Agriculture,1974,57:301-312.
[10]FUJINO K, SEKIGUCHI H, SATO T, et al. Mapping of quantitative trait loci controlling low-temperature germinability in rice (Oryza sativa L.)[J]. Theoretical and Applied Genetics,2004,108(5):794-799.
[11]侯名语,王春明,江玲,等.水稻低温发芽力QTL定位和遗传分析[J]. 遗传学报,2004(7):701-706.
[12]陈亮,楼巧君,孙宗修,等. 水稻低温发芽力的QTL定位[J]. 中国水稻科学,2006,20(2):159-164.
[13]杨梯丰,张子怡,董景芳,等. 水稻低温发芽力QTL qLTG3-1基因内分子标记的开发及其在华南籼稻中的应用评价[J]. 广东农业科学,2021,48(10):32-41.
[14]FUJINO K, SEKIGUCHI H, MATSUDA Y, et al. Molecular identification of a major quantitative trait locus,qLTG3-1,controlling low-temperature germinability in rice[J]. Proceedings of the National Academy of Sciences of the United States of America,2008,105(34):12623-12628.
[15]SHIM K C, KIM S H, LEE H S, et al. Characterization of a new qLTG3-1 allele for low-temperature germinability in rice from the wild species Oryza rufipogon[J]. Rice,2020,13(1):10.
[16]YANG T F, ZHOU L, ZHAO J L, et al. The candidate genes underlying a stably expressed QTL for low temperature germinability in rice (Oryza sativa L.)[J]. Rice,2020,13(1):74.
[17]SUH J P, AHN S N, MOON H P, et al. QTL analysis of low temperature geminability in a Korean weedy rice (Oryza sativa L.)[J]. Rice Research Newsletter,1999,16:53-55.
[18]HORI K, SUGIMOTO K, NONOUE Y, et al. Detection of quantitative trait loci controlling pre-harvest sprouting resistance by using backcrossed populations of Japonica rice cultivars[J]. Theoretical and Applied Genetics,2010,120(8):1547-1557.
[19]LI L Y, CHEN H P, MAO D H. Pyramiding of rapid germination loci from Oryza sativa cultivar ‘Xieqingzao B’ and cold tolerance loci from Dongxiang wild rice to increase climate resilience of cultivated rice[J]. Molecular Breeding,2019,39(6):85.
[20]滕胜, 曾大力, 钱前, 等. 低温条件下水稻发芽力 QTL 的定位分析[J]. 科学通报,2001,13:1104-1108.
[21]杨梯丰, 张少红, 赵均良, 等. 水稻耐冷QTL定位的比较分析[J]. 分子植物育种,2015,13(1):1-15.
[22]FUJINO K, SEKIGUCHI H, SATO T, et al. Mapping of quantitative trait loci controlling low-temperature germinability in rice (Oryza sativa L.)[J]. Theoretical and Applied Genetics,2004,108(5):794-799.
[23]李静,李雨欣,赵洁,等. 水稻穗发芽抗性鉴定方法优化及批量筛选[J]. 江西农业大学学报,2025,47(3):569-586.
[24]FINCH-SAVAGE W E, LEUBNER-METZGER G. Seed dormancy and the control of germination[J]. New Phytologist,2006,171(3):501-523.
[25]曹微, 王燕, 谭斌, 等. 水稻品种直播相关的种子低温和低氧萌发活力评价[J]. 分子植物育种,2018,16(10):3259-3268.
[26]景德道,刁立平,钱华飞,等. 水稻直播与移栽的比较及相应育种策略[J]. 江西农业学报,2008,20(7):17-20.
[27]汪向东,陈再高. 水稻直播生产现状及发展对策[J]. 现代农业科技,2019,15:49-51.
[28]BENTSINK L, KOORNNEEF M. Seed dormancy and germination[J]. The Arabidopsis Book,2008,6:e0119.
[29]LI C D, NI P X, FRANCKI M, et al. Genes controlling seed dormancy and pre-harvest sprouting in a rice-wheat-barley comparison[J]. Functional & Integrative Genomics,2004,4(2):84-93.
[30]CHEN Y, XIANG Z P, LIU M, et al. ABA biosynthesis gene OsNCED3 contributes to preharvest sprouting resistance and grain development in rice[J]. Plant,Cell & Environment,2023,46(4):1384-1401.
[31]CHEN W Q, WANG W, LYU Y S, et al. OsVP1 activates Sdr4 expression to control rice seed dormancy via the ABA signaling pathway[J]. The Crop Journal,2021,9(1):68-78.
[32]ZHAO B, ZHANG H, CHEN T X, et al. Sdr4 dominates pre-harvest sprouting and facilitates adaptation to local climatic condition in Asian cultivated rice[J]. Journal of Integrative Plant Biology,2022,64(6):1246-1263.
[33]XU F, TANG J Y, WANG S X, et al. Antagonistic control of seed dormancy in rice by two bHLH transcription factors[J]. Nature Genetics,2022,54(12):1972-1982.
[34]WANG X, ZOU B H, SHAO Q L, et al. Natural variation reveals that OsSAP16 controls low-temperature germination in rice[J]. Journal of Experimental Botany,2018,69(3):413-421.
[35]PAN Y H, LIANG H F, GAO L J, et al. Transcriptomic profiling of germinating seeds under cold stress and characterization of the cold-tolerant gene LTG5 in rice[J]. BMC Plant Biology,2020,20(1):371.
[36]田孟祥,张时龙,何友勋,等. 水稻耐低温基因bZIP73分子标记的开发与验证[J]. 江苏农业学报,2019,35(6):1265-1270.
[37]ZHANG C Z, WANG H R, TIAN X J, et al. A transposon insertion in the promoter of OsUBC12 enhances cold tolerance during japonica rice germination[J]. Nature Communications,2024,15(1):2211.

相似文献/References:

[1]王士磊,丁正权,黄海祥.水稻隐性早熟突变体ref早熟性的遗传分析和基因定位[J].江苏农业学报,2016,(04):721.[doi:10.3969/j.issn.100-4440.2016.04.001]
　WANG Shi-lei,DING Zheng-quan,HUANG Hai-xiang.Inheritance and gene mapping of recessive earliness in rice (Oryza sativa L.)[J].,2016,(06):721.[doi:10.3969/j.issn.100-4440.2016.04.001]
[2]王在满,郑乐,张明华,等.不同播种方式对直播水稻倒伏指数和根系生长的影响[J].江苏农业学报,2016,(04):725.[doi:10.3969/j.issn.100-4440.2016.04.002]
　WANG Zai-man,ZHENG Le,ZHANG Ming-hua,et al.Effects of seeding manners on lodging index and root growth of directseeded rice[J].,2016,(06):725.[doi:10.3969/j.issn.100-4440.2016.04.002]
[3]易能,薛延丰,石志琦,等.微囊藻毒素对水稻种子萌发和幼苗生长的胁迫作用[J].江苏农业学报,2016,(04):729.[doi:10.3969/j.issn.100-4440.2016.04.003]
　YI Neng,XUE Yan-feng,SHI Zhi-qi,et al.Inhibitory effect of microcystins on seed germination and seedling growth of rice[J].,2016,(06):729.[doi:10.3969/j.issn.100-4440.2016.04.003]
[4]刘凯,王爱民,严国红,等.一个水稻显性矮秆突变体的遗传特性与降株高能力[J].江苏农业学报,2016,(05):968.[doi:10.3969/j.issn.1000-4440.2016.05.002]
　LIU Kai,WANG Ai-min,YAN Guo-hong,et al.Genetic analysis and plant height reduction of a dominant dwarf mutant of rice[J].,2016,(06):968.[doi:10.3969/j.issn.1000-4440.2016.05.002]
[5]王红,杨镇,裴文琪,等.功能性微生物制剂对镉胁迫下水稻生长及生理特性的影响[J].江苏农业学报,2016,(05):974.[doi:10.3969/j.issn.1000-4440.2016.05.003]
　WANG Hong,YANG Zhen,PEI Wen-qi,et al.Growth and physiological characteristics of cadmium-stressed rice influenced by functional microorganism agent[J].,2016,(06):974.[doi:10.3969/j.issn.1000-4440.2016.05.003]
[6]孙玲,单捷,毛良君,等.基于遥感和Moran's I指数的水稻面积变化空间自相关性研究[J].江苏农业学报,2016,(05):1060.[doi:10.3969/j.issn.1000-4440.2016.05.017]
　SUN Ling,SHAN Jie,MAO Liang-jun,et al.Spatial autocorrelation of changes in paddy rice area based on remote sensing and Moran’s I index[J].,2016,(06):1060.[doi:10.3969/j.issn.1000-4440.2016.05.017]
[7]张晓忆,李卫国,景元书,等.多种光谱指标构建决策树的水稻种植面积提取[J].江苏农业学报,2016,(05):1060.[doi:10.3969/j.issn.1000-4440.2016.05.018]
　ZHANG Xiao-yi,LI Wei-guo,JING Yuan-shu,et al.Extraction of paddy rice area by constructing the decision tree with multiple spectral indices[J].,2016,(06):1060.[doi:10.3969/j.issn.1000-4440.2016.05.018]
[8]裔传灯,李玮,王德荣,等.水稻GW5基因的1212-bp Indel变异对粒形的影响[J].江苏农业学报,2016,(06):1201.[doi:doi:10.3969/j.issn.1000-4440.2016.06.001]
　YI Chuan-deng,LI Wei,WANG De-rong,et al.Effect of 1212-bp Indel variation of gene GW5 on rice grain shape[J].,2016,(06):1201.[doi:doi:10.3969/j.issn.1000-4440.2016.06.001]
[9]刘红江,陈虞雯,张岳芳,等.不同播栽方式对水稻叶片光合特性及产量的影响[J].江苏农业学报,2016,(06):1206.[doi:doi:10.3969/j.issn.1000-4440.2016.06.002]
　LIU Hong-jiang,CHEN Yu-wen,ZHANG Yue-fang,et al.Effects of planting pattern on leaf photosynthetic characteristics and yield of rice[J].,2016,(06):1206.[doi:doi:10.3969/j.issn.1000-4440.2016.06.002]
[10]郭保卫,许轲,魏海燕,等.钵苗机插水稻茎秆的抗倒伏能力[J].江苏农业学报,2016,(06):1280.[doi:doi:10.3969/j.issn.1000-4440.2016.06.014]
　GUO Bao-wei,XU Ke,WEI Hai-yan,et al.Culm lodging resistance characteristics of bowl seedling mechanical-transplanting rice[J].,2016,(06):1280.[doi:doi:10.3969/j.issn.1000-4440.2016.06.014]

备注/Memo

备注/Memo:
收稿日期:2025-05-26基金项目:江苏省农业科技自主创新基金项目[CX(23)1001]；江苏省重点研发计划(现代农业)项目(BE2021359)；国家自然科学基金项目(31571585)；江苏省种业创新”揭榜挂帅”项目[JBGS(2021)038、JBGS(2021)039)]；生物育种钟山实验室项目(BM2022008-03、ZSBBL-KY2023-01、ZSBBL-KY2003-06-1-2、ZSBBL-KY2024-01)；国际合作重点项目(2023YFE01117000)作者简介:李雨欣(2001-),女,江苏宿迁人,硕士研究生,研究方向为作物遗传育种。(E-mail)1311807704@qq.com通讯作者:李霞,(E-mail)jspplx@jaas.ac.cn
更新日期/Last Update: 2026-07-15