[1]田莉莉,马丽雅,万群,等.老化处理对纳米塑料在作物中的积累、转运及亚细胞分布的影响[J].江苏农业学报,2026,42(06):1092-1103.[doi:doi:10.3969/j.issn.1000-4440.2026.06.002]
　TIAN Lili,MA Liya,WAN Qun,et al.Effects of aging treatment on the accumulation, translocation, and subcellular distribution of nanoplastics in crops[J].,2026,42(06):1092-1103.[doi:doi:10.3969/j.issn.1000-4440.2026.06.002]
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老化处理对纳米塑料在作物中的积累、转运及亚细胞分布的影响()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年06期
页码:
1092-1103
栏目:
微塑料的环境效应和健康影响专题
出版日期:
2026-06-30

文章信息/Info

Title:
Effects of aging treatment on the accumulation, translocation, and subcellular distribution of nanoplastics in crops
作者:
田莉莉1; 马丽雅1; 万群1; 程金金1; 王亚1; 季荣2; 余向阳1
(1.江苏省农业科学院农产品质量安全与营养研究所,省部共建国家重点实验室培育基地-江苏省食品质量安全重点实验室,江苏南京210014；2.南京大学环境学院,水污染控制与资源绿色循环全国重点实验室,江苏南京210023)
Author(s):
TIAN Lili1; MA Liya1; WAN Qun1; CHENG Jinjin1; WANG Ya1; JI Rong2; YU Xiangyang1
(1.Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China；2.State Key Laboratory of Water Pollution Control and Green Resource Recycling, School of Environment, Nanjing University, Nanjing 210023, China)
关键词:
微塑料; 纳米塑料; 光老化; 积累; 转运; 亚细胞分布; 聚苯乙烯
Keywords:
microplastics; nanoplastics; photo-aging; accumulation; translocation; subcellular distribution; polystyrene
分类号:
X592
DOI:
doi:10.3969/j.issn.1000-4440.2026.06.002
文献标志码:
A
摘要:
纳米塑料在农田土壤-作物系统中的环境行为及生物效应备受关注。老化纳米塑料的理化性质会发生改变,进而影响其在作物体内的归趋,但影响机制尚不明确。本研究通过紫外光加速老化处理制备具有环境特征的聚苯乙烯(PS)纳米塑料,并利用14C同位素示踪技术,系统对比了原始14C-PS纳米塑料和老化14C-PS纳米塑料在水稻幼苗和青菜中的积累、转运及亚细胞分布的差异。结果表明,原始14C-PS纳米塑料和老化14C-PS纳米塑料均主要积累于根部,向地上部的转运系数较低；但与原始14C-PS纳米塑料处理相比,老化处理促进了14C-PS纳米塑料从根部向茎叶的转运,且在水培条件下促进效应更明显。亚细胞分布结果表明,原始14C-PS纳米塑料主要积累于根部细胞壁(>98.0%),而老化14C-PS纳米塑料在细胞器和可溶性组分中的占比明显增加,表明老化处理改变了14C-PS纳米塑料在细胞层面的分布特征。抑制剂试验结果表明,原始14C-PS纳米塑料在作物体内的积累不受抑制剂的显著影响,而老化14C-PS纳米塑料在作物体内的积累则表现出明显的能量依赖性,并且受到水通道蛋白调控。本研究结果可以为评估老化纳米塑料的环境风险与食品安全风险提供科学依据。
Abstract:
The environmental behavior and biological effects of nanoplastics (NPs) in soil-crop systems have attracted significant attention. The aging can significantly alter the physicochemical properties of nanoplastics, thereby influencing their fate in crops; however, the underlying mechanisms remain unclear. In this study, polystyrene (PS) nanoplastics with environmentally relevant characteristics were prepared through ultraviolet (UV)-accelerated aging, and the differences in the accumulation, translocation, and subcellular distribution of pristine and photo-aged PS-NPs were systematically compared in rice seedlings and pak choi using 14C isotopic tracing technology. The results revealed that both PS-NPs primarily accumulated in the roots, with low translocation factors to aboveground tissues. Compared with pristine 14C-PS-NPs, aging treatment enhanced the translocation of 14C-PS-NPs from roots to shoots, and this promoting effect was more pronounced under hydroponic conditions. Subcellular distribution analysis revealed that pristine 14C-PS-NPs mainly accumulated in the root cell walls (>98.0%), whereas aged 14C-PS-NPs exhibited a significantly increased proportion in the organelles and soluble fractions, indicating that aging altered the subcellular distribution patterns of 14C-PS-NPs. Inhibitor assay results demonstrated that the accumulation of pristine PS-NPs was insensitive to the inhibitors, whereas the accumulation of aged 14C-PS-NPs exhibited significant energy dependence and was regulated by aquaporins. The results of this study can provide a scientific basis for assessing the environmental risks and food safety risks of aged nanoplastics.

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备注/Memo

备注/Memo:
收稿日期:2026-03-07基金项目:江苏省自然科学基金项目(BK20230755)；国家自然科学基金项目(32402421)作者简介:田莉莉(1988-),女,河南周口人,博士,副研究员,主要从事新污染物环境行为与效应研究。(E-mail)tianlili@jaas.ac.cn通讯作者:余向阳,(E-mail)yuxy@jaas.ac.cn
更新日期/Last Update: 2026-07-15