[1]陈龙,常小丽,胡建军,等.春尺蠖2个小热激蛋白编码基因的克隆及其在不同时空和温度胁迫下的表达情况[J].江苏农业学报,2026,42(04):691-700.[doi:doi:10.3969/j.issn.1000-4440.2026.04.005]
　CHEN Long,CHANG Xiaoli,HU Jianjun,et al.Cloning, temporal-spatial expression, and temperature stress response of two small heat shock protein genes in Apocheima cinerarius (Lepidoptera:Geometridae)[J].,2026,42(04):691-700.[doi:doi:10.3969/j.issn.1000-4440.2026.04.005]
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春尺蠖2个小热激蛋白编码基因的克隆及其在不同时空和温度胁迫下的表达情况()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年04期
页码:
691-700
栏目:
植物保护
出版日期:
2026-04-30

文章信息/Info

Title:
Cloning, temporal-spatial expression, and temperature stress response of two small heat shock protein genes in Apocheima cinerarius (Lepidoptera:Geometridae)
作者:
陈龙1; 2; 常小丽3; 胡建军4; 王克秀4; 程明军5; 越慧芳6; 白艳7; 崔阔澍8
(1.河套学院农学系,内蒙古巴彦淖尔015000;2.河套地区绿色农产品安全生产与预警控制实验室,内蒙古巴彦淖尔015000;3.四川农业大学,四川成都611130；4.四川省农业科学院作物研究所<四川省种质资源中心>,四川成都610066)
Author(s):
CHEN Long1; 2; CHANG Xiaoli3; HU Jianjun4; WANG Kexiu4; CHENG Mingjun5; YUE Huifang6; BAI Yan7; CUI Kuoshu8
(1.Agriculture Department, Hetao College, Bayannur 015000, China;2.Hetao Area Green Agricultural Product Safety Production and Early Warning Control Laboratory, Bayannur 015000, China;3.Sichuan Agricultural University, Chengdu 611130, China；4.Crop Research Institute, Sichuan Academy of Agricultural Sciences (Sichuan Province Germplasm Resource Center), Chengdu 610066, China)
关键词:
春尺蠖; 小分子热激蛋白; 滞育; 逆境胁迫; 表达谱
Keywords:
Apocheima cinerarius; small heat shock protein; diapause; adverse stress; expression profile
分类号:
S888.72+1
DOI:
doi:10.3969/j.issn.1000-4440.2026.04.005
文献标志码:
A
摘要:
本研究旨在克隆春尺蠖(Apocheima cinerarius)中2个小热激蛋白编码基因(sHSP),分析其在不同发育阶段、不同组织、不同温度胁迫下的表达模式,以期明确小热激蛋白在春尺蠖应答温度胁迫过程中的作用。基于春尺蠖蛹的转录组数据,筛选并克隆春尺蠖的2个sHSP基因,对其进行系统发育分析、结构域分析等生物信息学分析。用实时荧光定量PCR(qPCR)技术检测sHSP基因在春尺蠖不同发育阶段(卵、幼虫、蛹及成虫)及不同组织(头部、胸部、腹部、足部及翅膀)中的时空表达特征,并进一步分析其4龄幼虫在不同温度(-5 ℃、0 ℃、5 ℃、25 ℃和30 ℃)胁迫下的表达模式。克隆获取2个sHSP基因后,分别将其命名为AcinHSP20.4、AcinsHSP(GenBank登录号分别为PV053122和PV053123),编码序列(CDS)全长分别为543 bp、758 bp,分别编码180个、252个氨基酸,相对分子量分别为20 470、27 758,理论等电点分别为6.98、4.60。基因表达结果显示,AcinHSP20.4基因在春尺蠖卵至5龄幼虫阶段的相对表达量较低,在滞育解除蛹期的相对表达量最高；AcinsHSP基因的相对表达量在春尺蠖卵至5龄幼虫阶段处于较低水平,在雌虫中的相对表达量达到最大值。AcinHSP20.4基因的相对表达量在雌虫胸部最高,AcinsHSP基因的相对表达量则在雄虫头部最高。随着温度的升高,AcinHSP20.4基因的相对表达量整体表现为先升高后降低的趋势,在25 ℃达到最大值,在30 ℃时下降并且与25 ℃的相对表达量间表现出显著差异(P＜0.05)；AcinsHSP基因的相对表达量总体呈上升趋势,在0～30 ℃范围内无显著差异,在-5 ℃与30 ℃之间差异显著(P＜0.05)。由研究结果可以看出,sHSP基因在春尺蠖生长发育、滞育及响应温度胁迫的过程中具有重要作用,研究结果为进一步探究春尺蠖生长发育机制、滞育机制及对温度胁迫的适应性机制奠定了一定的理论基础。
Abstract:
This study aimed to clone two small heat-shock protein genes (sHSPs) from Apocheima cinerarius and to analyse their expression patterns across different developmental stages, tissues and temperature stresses, so as to clarify the role of sHSPs in the response of A. cinerarius to temperature stress. Based on the pupal transcriptome of A. cinerarius, two sHSP genes were screened and cloned, and subjected to phylogenetic and domain-related bioinformatic analyses. Quantitative real-time PCR (qPCR) was used to examine the spatiotemporal expression characteristics of the two sHSP genes in different developmental stages (egg, larva, pupa and adult) and tissues (head, thorax, abdomen, legs and wings) of A. cinerarius, and to analyse their expression profiles in 4th-instar larvae exposed to temperatures of -5 ℃, 0 ℃, 5 ℃, 25 ℃ and 30 ℃. The two cloned sHSP genes were designated AcinHSP20.4 and AcinsHSP (GenBank accession numbers PV053122 and PV053123, respectively); their coding sequences (CDS) were 543 bp and 758 bp in length, encoding 180 and 252 amino acids with predicted molecular masses of 20 470 and 27 758 and theoretical isoelectric points of 6.98 and 4.60, respectively. Expression analysis revealed that AcinHSP20.4 transcript levels were low from the egg to the 5th-instar larval stage and peaked in diapause-terminated pupae, whereas AcinsHSP transcript levels remained low from the egg to the 5th-instar larval stage and reached their maximum in female adults. The relative expression level of AcinHSP20.4 was the highest in the thorax of female adults, while that of AcinsHSP was the highest in the head of male adults. As temperature increased, the relative expression of AcinHSP20.4 first increased and then decreased, peaking at 25 ℃ and decreasing significantly at 30 ℃ (P＜0.05). The relative expression of AcinsHSP showed an overall upward trend, with no significant difference within the temperature range of 0-30 ℃, but a significant difference was observed between -5 ℃ and 30 ℃ (P＜0.05). These results indicate that sHSP genes play important roles in the growth, development, diapause and temperature-stress response of A. cinerarius, and provide a theoretical basis for further investigation into the mechanisms underlying its development, diapause and adaptation to temperature stress.

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备注/Memo

备注/Memo:
收稿日期：2025-05-26基金项目：内蒙古自然科学基金项目(2021BS03028)；四川省中央“三区”科技人才支持计划项目；四川省饲草创新团队项目(sccxtd-2024-16)作者简介：陈龙(1988-),男,陕西汉中人,博士,副教授,主要从事昆虫生态与分子生物学研究。(E-mail)15774711140@163.com通讯作者：崔阔澍,(E-mail)cuikuoshu@126.com
更新日期/Last Update: 2026-05-11