[1]张诗琦,朱守晶,胡能兵.小麦对缺锌胁迫响应的转录组分析[J].江苏农业学报,2026,42(04):670-681.[doi:doi:10.3969/j.issn.1000-4440.2026.04.003]
　ZHANG Shiqi,ZHU Shoujing,HU Nengbing.Transcriptomic analysis of wheat in response to zinc deficiency stress[J].,2026,42(04):670-681.[doi:doi:10.3969/j.issn.1000-4440.2026.04.003]
点击复制

小麦对缺锌胁迫响应的转录组分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年04期
页码:
670-681
栏目:
遗传育种·生理生化
出版日期:
2026-04-30

文章信息/Info

Title:
Transcriptomic analysis of wheat in response to zinc deficiency stress
作者:
张诗琦; 朱守晶; 胡能兵
(安徽科技工程大学,安徽凤阳233100)
Author(s):
ZHANG Shiqi; ZHU Shoujing; HU Nengbing
(Anhui Science and Technology University, Fengyang 233100, China)
关键词:
小麦; 缺锌胁迫; 转录组分析; GO分析; KEGG分析
Keywords:
Triticum aestivum L.; zinc deficiency stress; transcriptome analysis; GO analysis; KEGG analysis
分类号:
S512.1
DOI:
doi:10.3969/j.issn.1000-4440.2026.04.003
文献标志码:
A
摘要:
锌(Zn)是动植物生长发育必需的微量营养元素之一,生物机体只能通过从外界摄入获取。小麦是世界三大主要粮食作物之一,更是部分Zn营养缺乏地区的主要粮食作物。通过分子设计育种提高小麦籽粒Zn含量,是改善这些地区 “隐形饥饿” 问题的高效方式。本研究在对多份小麦种质进行锌积累能力比较的基础上,选取锌高效品种中麦175进行缺锌胁迫处理,发现与不缺锌胁迫对照相比，其总根长、根体积、根表面积、平均根直径和根尖数量等在缺锌胁迫初期均显著增加。转录组分析结果表明,缺锌胁迫下中麦175的根系和叶片中分别有1 936个和2 287个差异表达基因(DEG)。GO分析结果显示,这些差异表达基因主要富集于离子跨膜转运蛋白活性、离子稳态、金属离子转运、锌离子转运及植物激素信号通路等条目。KEGG分析结果表明,叶片中差异表达基因主要富集在半胱氨酸和甲硫氨酸代谢通路,其中参与麦根酸类物质(Mugineic acids,MA)生物合成的SAM、NAAT等基因,以及参与乙烯合成的ACO、ACS基因均显著上调表达；根系中差异表达基因主要富集在苯丙烷生物合成通路,其中参与木质素合成的关键基因(如CCR、CAD、COMT、LAC基因)均显著下调表达。推测中麦175在缺锌环境下可能通过降低细胞壁滞留金属离子的能力,以提升根系对Zn离子的吸收效率。本研究结果解析了小麦Zn吸收与积累的分子机制,为小麦锌生物强化育种提供了基因资源。
Abstract:
Zinc (Zn) is one of the essential micronutrients for the growth and development of animals and plants, and living organisms can only acquire it through external intake. Wheat is one of the three major staple food crops globally, and even serves as the primary staple food crop in regions deficient in Zn nutrition. Enhancing Zn content in wheat grains via molecular design breeding is an efficient strategy to alleviate the issue of hidden hunger in these areas. Based on comparing Zn accumulation capacity among multiple wheat germplasm accessions, this study selected Zhongmai 175, a wheat variety with high Zn accumulation efficiency, for Zn deficiency stress treatment. It was found that compared with the control (without Zn deficiency), indicators including total root length, root volume, root surface area, average root diameter, and number of root tips significantly increased in the early stage of stress. Further transcriptome analysis revealed that under Zn deficiency stress, there were 1 936 and 2 287 differentially expressed genes (DEGs) in the roots and leaves of Zhongmai 175, respectively. GO analysis showed these DEGs were mainly enriched in terms such as ion transmembrane transporter activity, ion homeostasis, metal ion transport, zinc ion transport, and plant hormone signaling pathways. KEGG analysis indicated that DEGs in leaves were primarily enriched in the cysteine and methionine metabolism pathways. Among them, genes involved in the biosynthesis of mugineic acids (e.g., SAM and NAAT) and genes related to ethylene synthesis (e.g., ACO and ACS) were significantly up-regulated. DEGs in roots were mainly enriched in the phenylpropanoid biosynthesis pathway, and key genes involved in lignin synthesis (e.g., CCR, CAD, COMT, and LAC genes) were significantly down-regulated. It is hypothesized that Zhongmai 175 may improve the absorption efficiency of Zn ions in roots by reducing the ability of cell walls to retain metal ions under Zn-deficient conditions. The results of this study provide genetic resources for deciphering the molecular mechanisms of Zn absorption and accumulation in wheat, as well as for Zn biofortification breeding.

参考文献/References:

[1]HOU R, HE Y, YAN G, et al. Zinc enzymes in medicinal chemistry[J]. European Journal of Medicinal Chemistry,2021,226:113877.
[2]李莎莎,王朝辉,刁超朋,等. 旱地高产小麦品种籽粒锌含量差异与产量构成和锌吸收利用的关系[J]. 植物营养与肥料学报,2018,24(4):849-856.
[3]KUMSSA D B, JOY E J M, LOUISE ANDER E, et al. Dietary calcium and zinc deficiency risks are decreasing but remain prevalent[J]. Scientific Reports,2015,5:10974.
[4]张明艳,郁一凡,封超年,等. 不同基因型小麦籽粒、面粉和麸皮中Ca和Zn含量的差异[J]. 麦类作物学报,2011,31(2):240-245.
[5]刘慧. 我国主要麦区小麦籽粒产量和关键营养元素含量评价及调控[D]. 杨凌:西北农林科技大学,2016.
[6]UAUY C, DISTELFELD A, FAHIMA T, et al. A NAC gene regulating senescence improves grain protein,zinc,and iron content in wheat[J]. Science,2006,314(5803):1298-1301.
[7]BHATI K K, ALOK A, KUMAR A, et al. Silencing of ABCC13 transporter in wheat reveals its involvement in grain development, phytic acid accumulation and lateral root formation[J]. Journal of Experimental Botany,2016,67(14):4379-4389.
[8]ZULFIQAR U, KHOKHAR A, MAQSOOD M F, et al. Genetic biofortification: advancing crop nutrition to tackle hidden hunger[J]. Functional & Integrative Genomics,2024,24(2):34.
[9]OLSEN L I, HANSEN T H, LARUE C, et al. Mother-plant-mediated pumping of zinc into the developing seed[J]. Nature Plants,2016,2(5):16036.
[10]ABU-ABIED M, ROGOVOY STELMAKH O, MORDEHAEV I, et al. Dissecting the contribution of microtubule behaviour in adventitious root induction[J]. Journal of Experimental Botany,2015,66(9):2813-2824.
[11]CHOI Y, LEE Y, KIM S Y, et al. Arabidopsis ROP-interactive CRIB motif-containing protein 1 (RIC1) positively regulates auxin signalling and negatively regulates abscisic acid (ABA) signalling during root development[J]. Plant Cell & Environment,2013,36(5):945-955.
[12]黄俊丽,马娜娜,车树刚,等. 植物叶脉发育的分子机制[J]. 生命科学,2011,23(8):804-811.
[13]WELCH R M, GRAHAM R D. Breeding for micronutrients in staple food crops from a human nutrition perspective[J]. Journal of Experimental Botany,2004,55(396):353-364.
[14]BROWN P H, WELCH R M, CARY E E. Nickel:a micronutrient essential for higher plants[J]. Plant Physiology,1987,85(3):801-803.
[15]HART J J, WELCH R M, NORVELL W A, et al. Characterization of cadmium binding, uptake, and translocation in intact seedlings of bread and durum wheat cultivars[J]. Plant Physiology,1998,116 (4):1413-1420.
[16]DANESHBAKHSH B, KHOSHGOFTARMANESH A H, SHARIATMADARI H, et al. Phytosiderophore release by wheat genotypes differing in zinc deficiency tolerance grown with Zn-free nutrient solution as affected by salinity[J]. Journal of Plant Physiology,2013,170(1):41-46.
[17]YOUSFI S, RABHI M, ABDELLY C, et al. Iron deficiency tolerance traits in wild (Hordeum maritimum) and cultivated barley (Hordeum vulgare) [J]. Comptes Rendus Biologies,2009,332(6):523-533.
[18]WISSUWA M, ISMAIL A M, YANAGIHARA S. Effects of zinc deficiency on rice growth and genetic factors contributing to tolerance[J]. Plant Physiology,2006,142(2):731-741.
[19]CAFFALL K H, MOHNEN D. The structure, function, and biosynthesis of plant cell wall pectic polysaccharides[J]. Carbohydrate Research,2009,344(14):1879-1900.
[20]VON WIREN N, MARSCHNER H, ROMHELD V. Roots of iron-efficient maize also absorb phytosiderophore-chelated zinc[J]. Plant Physiology,1996,111(4):1119-1125.
[21]MARTINOIA E. Vacuolar transporters-companions on a longtime journey[J]. Plant Physiology,2018,176(2):1384-1407.
[22]王玉,杨雪,杨蕊菁,等. 调控苯丙烷类生物合成的MYB类转录因子研究进展[J]. 安徽农业大学学报,2019,46(5):859-864.
[23]穆红梅,杜秀菊,张秀省,等. 植物MYB转录因子调控苯丙烷类生物合成研究[J]. 北方园艺,2015(24):171-174.
[24]王星,罗双霞,于萍,等. 茄科蔬菜苯丙烷类代谢及相关酶基因研究进展[J]. 园艺学报,2017,44(9):1738-1748.
[25] RANADE S S, SEIPEL G, GORZSS A, et al. Enhanced lignin synthesis and ecotypic variatio in defense-related gene expression im response to shade in Norway spruce［J］. Plant,Cell & Environment，2022，45(9)：2671-2681.
[26]CHENG Z, WANG C, TANG F, et al. The cell wall functions in plant heavy metal response[J]. Ecotoxicology and Environmental Safety,2025,299:118326.
[27]WAKABAYASHI K, SOGA K, HOSON T, et al. The modification of cell wall properties is involved in the growth inhibition of rice coleoptiles induced by lead stress[J]. Life(Basel),2023,13(2):471.
[28]SHANGGUAN X, QI Y, WANG A, et al. OsGLP participates in the regulation of lignin synthesis and deposition in rice against copper and cadmium toxicity[J]. Frontiers in Plant Science,2023,13:107811.
[29]PARROTTA L, GUERRIERO G, SERGEANT K, et al. Target or barrier? The cell wall of early- and later-diverging plants vs cadmium toxicity: differences in the response mechanisms[J]. Frontiers in Plant Science,2015,6:133.
[30]THOLL D. Biosynthesis and biological functions of terpenoids in plants[J]. Advances in Biochemical Engineering/Biotechnology,2015,148:63-106.
[31]GIORDANO A, LIU Z Q, PANTER S N, et al. Reduced lignin content and altered lignin composition in the warm season forage grass Paspalum dilatatum by down-regulation of a Cinnamoyl CoA Reductase Gene[J]. Transgenic Research,2014,23(3):503-517.
[32]ZHOU J, LEE C, ZHONG R, et al. MYB58 and MYB63 are transcriptional activators of the lignin biosynthetic pathway during secondary cell wall formation in Arabidopsis[J]. The Plant Cell,2009,21(1):248-266.
[33]GENG P, ZHANG S, LIU J, et al. MYB20, MYB4, MYB4, and MYB85 regulate phenylalanine and lignin biosynthesis during secondary cell wall formation[J]. Plant Physiology,2020,182(3):1272-1283.
[34]李媛,陈金焕,金曌,等. 毛果杨NAC128基因在次生壁形成中的功能[J]. 林业科学,2020,56(11):62-72.
[35]NOZOYE T, NAGASAKA S, KOBAYASHI T, et al. Phytosiderophore efflux transporters are crucial for iron acquisition in graminaceous plants[J]. Journal of Biological Chemistry,2011,286(7):5446-5454.
[36]NING X Y, LIN M F, HUANG G H, et al. Research progress on iron absorption, transport, and molecular regulation strategy in plants[J]. Frontiers in Plant Science,2023,14:1190768.
[37]LEE S, RAHMAN M M, NAKANISHI H, et al. Concomitant activation of OsNAS2 and OsNAS3 contributes to the enhanced accumulation of iron and zinc in rice[J]. International Journal of Molecular Sciences,2023,24(7):6568.
[38]SCHAAF G, LUDEWIG U, ERENOGLU B E, et al. ZmYS1 functions as a proton-coupled symporter for phytosiderophore-and nicotianamine-chelated metals[J]. Journal of Biological Chemistry,2004,279(10):9091-9096.
[39]SUZUKI M, TAKAHASHI M, TSUKAMOTO T, et al. Biosynthesis and secretion of mugineic acid family phytosiderophores in zinc-deficient barley[J]. The Plant Journal,2006,48(1):85-97.
[40]YOUSFI S, WISSAL M, MAHMOUDI H, et al. Effect of salt on physiological responses of barley to iron deficiency[J]. Plant Physiology and Biochemistry,2007,45(5):309-314.
[41]WANG X Y, WEN H Y, SUPRUN A, et al. Ethylene signaling in regulating plant growth,development,and stress responses[J]. Plants,2025,14(3):309.
[42]ZHAO X C, QU X, MATHEWS D E, et al. Effect of ethylene pathway mutations upon expression of the ethylene receptor ETR1 from Arabidopsis[J]. Plant Physiology,2002,130(4):1983-1991.
[43]WANG K L, LI H, ECKER J R. Ethylene biosynthesis and signaling networks[J]. The Plant Cell,2002,14(Suppl):131-151.
[44]BOOKER M A, DELONG A. Producing the ethylene signal: regulation and diversification of ethylene biosynthetic enzymes[J]. Plant Physiology,2015,169(1):42-50.
[45]BLEECKER A B, KENDE H. Ethylene: a gaseous signal molecule in plants[J]. Annual Review of Cell and Developmental Biology,2000,16:1-18.
[46]YU Y B, ADAMS D O, YANG S F. 1-Aminocyclopropanecarboxylate synthase, a key enzyme in ethylene biosynthesis[J]. Archives of Biochemistry and Biophysics,1979,198(1):280-286.
[47]PATTYN J, VAUGHAN-HIRSCH J, VAN DE POEL B. The regulation of ethylene biosynthesis: a complex multilevel control circuitry[J]. The New Phytologist,2021,229(2):770-782.
[48]HOUBEN M, VAN DE POEL B. 1-aminocyclopropane-1-carboxylic acid oxidase (ACO): the enzyme that makes the plant hormone ethylene[J]. Frontiers in Plant Science,2019,10:695.
[49]LASSERRE E, BOUQUIN T, HERNANDEZ J A, et al. Structure and expression of three genes encoding ACC oxidase homologs from melon (Cucumis melo L.) [J]. Molecular & General Genetics,1996,251(1):81-90.
[50]JIA H, CHEN S, LIU D, et al. Ethylene-induced hydrogen sulfide negatively regulates ethylene biosynthesis by persulfidation of ACO in tomato under osmotic stress[J]. Frontiers in Plant Science,2018,9:1517.
[51]AHMADIZADEH M, CHEN J T, HASANZADEH S, et al. Insights into the genes involved in the ethylene biosynthesis pathway in Arabidopsis thaliana and Oryza sativa[J]. Journal of Genetic Engineering and Biotechnology,2020,18(1):62.
[52]MORAN LAUTER A N, PEIFFER G A, YIN T, et al. Identification of candidate genes involved in early iron deficiency chlorosis signaling in soybean (Glycine max) roots and leaves[J]. BMC Genomics,2014,15:702.

相似文献/References:

[1]伍 宏,朱昌华,夏 凯,等.叶面喷施激动素对小麦品种济麦22品质的影响[J].江苏农业学报,2016,(02):299.[doi:10.3969/j.issn.1000-4440.2016.02.010]
　WU Hong,ZHU Chang-hua,XIA Kai,et al.Effect of foliar application of kinetin on quality of Triticum aestivum L. Jimai 22[J].,2016,(04):299.[doi:10.3969/j.issn.1000-4440.2016.02.010]
[2]蒋正宁,别同德,赵仁惠,等.受条锈菌诱导的小麦丝氨酸苏氨酸激酶基因TaS/TK的克隆与表达[J].江苏农业学报,2016,(05):980.[doi:10.3969/j.issn.1000-4440.2016.05.004]
　JIANG Zheng-ning,BIE Tong-de,ZHAO Ren-hui,et al.Cloning and expression analysis of a Serine/Threonine protein kinase gene TaS/TK in wheat in response to stripe rust fungal infection[J].,2016,(04):980.[doi:10.3969/j.issn.1000-4440.2016.05.004]
[3]丁彬彬,张旭,吴磊,等.小麦3B 短臂染色体抗赤霉病主效 QTL 区域候选基因的表达[J].江苏农业学报,2017,(01):6.[doi:10.3969/j.issn.1000-4440.2017.01.002 ]
　DING Bin-bin,ZHANG Xu,WU Lei,et al.Expression of candidate genes on the region of a major QTL for the resistance to Fusarium head blight on the short arm of chromosome 3B in wheat[J].,2017,(04):6.[doi:10.3969/j.issn.1000-4440.2017.01.002 ]
[4]周淼平,姚金保,张鹏,等.小麦幼苗纹枯病抗性评价新方法[J].江苏农业学报,2017,(01):61.[doi:10.3969/j.issn.1000-4440.2017.01.010 ]
　ZHOU Miao-ping,YAO Jin-bao,ZHANG Peng,et al.New method for the resistance evaluation of wheat sharp eyespot in seedling[J].,2017,(04):61.[doi:10.3969/j.issn.1000-4440.2017.01.010 ]
[5]吴磊,姜朋,张瑜,等.苏麦3号小麦穗部病毒诱导的基因沉默（VIGS）体系的建立及验证[J].江苏农业学报,2017,(02):248.[doi:doi:10.3969/j.issn.1000-4440.2017.02.002]
　WU Lei,JIANG Peng,ZHANG Yu,et al.Construction and validation of virus-induced gene silencing(VIGS) system in spike of wheat variety Sumai 3[J].,2017,(04):248.[doi:doi:10.3969/j.issn.1000-4440.2017.02.002]
[6]邵继锋,陈荣府,董晓英,等.利用分根技术研究小麦铝磷交互作用[J].江苏农业学报,2016,(01):78.[doi:10.3969/j.issn.1000-4440.2016.01.012 ]
　SHAO Ji-feng,CHEN Rong-fu,DONG Xiao-ying,et al.Aluminum-phosphorus interaction in wheat grown in a split-root device[J].,2016,(04):78.[doi:10.3969/j.issn.1000-4440.2016.01.012 ]
[7]叶景秀.小麦籽粒蛋白质双向电泳体系的优化[J].江苏农业学报,2015,(05):957.[doi:doi:10.3969/j.issn.1000-4440.2015.05.002]
　YE Jing-xiu.Optimization of two-dimensional electrophresis system for grain protein in spring wheat[J].,2015,(04):957.[doi:doi:10.3969/j.issn.1000-4440.2015.05.002]
[8]郑舒文,徐其隆,邹华文.脱落酸对涝渍胁迫下小麦产量的影响[J].江苏农业学报,2015,(05):967.[doi:doi:10.3969/j.issn.1000-4440.2015.05.004]
　ZHENG Shu-wen,XU Qi-long,ZOU Hua-wen.Yield of waterlogged wheat in response to ABA application[J].,2015,(04):967.[doi:doi:10.3969/j.issn.1000-4440.2015.05.004]
[9]张玉萍,马占鸿.不同施氮量下小麦遥感估产模型构建[J].江苏农业学报,2015,(06):1325.[doi:doi:10.3969/j.issn.1000-4440.2015.06.020]
　ZHANG Yu-ping,MA Zhan-hong.Yield estimation model of wheat based on remote sensing data under different nitrogen supply conditions[J].,2015,(04):1325.[doi:doi:10.3969/j.issn.1000-4440.2015.06.020]
[10]张卓亚,王晓琳,许晓明,等.腐植酸对小麦扬花期水分利用效率及灌浆进程的影响[J].江苏农业学报,2015,(04):725.[doi:10.3969/j.issn.1000-4440.2015.04.003]
　ZHANG Zhuo-ya,WANG Xiao-ling,XU Xiao-ming,et al.Effect of humic acid on water use efficiency and grouting process of wheat at flowering[J].,2015,(04):725.[doi:10.3969/j.issn.1000-4440.2015.04.003]

备注/Memo

备注/Memo:
收稿日期：2025-05-08基金项目：安徽省高校自然科学研究项目(2023AH051846、2022AH051626)；安徽省高校理工科教师赴企业挂职实践计划项目(2024jsqygz68)作者简介：张诗琦(2000-),女,吉林辽源人,硕士研究生,主要从事作物遗传育种研究。(E-mail)411526105@qq.com通讯作者：朱守晶,(E-mail)zhusj@ahstu.edu.cn；胡能兵,(E-mail)hunb@ahstu.edu.cn
更新日期/Last Update: 2026-05-11