[1]杨宙,邹国兴,韩瑞才,等.OsCERK1DY基因导入籼稻中与丛枝菌根（AM）真菌的共生效应[J].江苏农业学报,2026,42(04):659-669.[doi:doi:10.3969/j.issn.1000-4440.2026.04.002]
　YANG Zhou,ZOU Guoxing,HAN Ruicai,et al.Symbiotic effect of the OsCERK1DY gene introduced into indica rice with arbuscular mycorrhizal (AM) fungi[J].,2026,42(04):659-669.[doi:doi:10.3969/j.issn.1000-4440.2026.04.002]
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OsCERK1DY基因导入籼稻中与丛枝菌根（AM）真菌的共生效应()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年04期
页码:
659-669
栏目:
遗传育种·生理生化
出版日期:
2026-04-30

文章信息/Info

Title:
Symbiotic effect of the OsCERK1DY gene introduced into indica rice with arbuscular mycorrhizal (AM) fungi
作者:
杨宙; 邹国兴; 韩瑞才; 朱珊; 汤国平; 邓伟; 黄仁良
(江西省农业科学院水稻研究所/水稻国家工程研究中心<南昌>/农业农村部双季稻种质创新与育种重点实验室<部省共建>,江西南昌330200)
Author(s):
YANG Zhou; ZOU Guoxing; HAN Ruicai; ZHU Shan; TANG Guoping; DENG Wei; HUANG Renliang
(Rice Research Institute, Jiangxi Academy of Agricultural Sciences/National Engineering Research Center of Rice (Nanchang)/Key Laboratory of Germplasm Innovation and Breeding of Double-cropping Rice (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Nanchang 330200, China)
关键词:
OsCERK1DY基因; 丛枝菌根（AM）真菌; 籼稻; 共生效应
Keywords:
OsCERK1DY gene; arbuscular mycorrhizal (AM) fungi; indica rice; symbiotic effect
分类号:
S511.2+1
DOI:
doi:10.3969/j.issn.1000-4440.2026.04.002
文献标志码:
A
摘要:
本研究以籼稻恢复系春恢350为对照,以导入丛枝菌根(Arbuscular mycorrhizae, AM)真菌高效共生基因OsCERK1DY的单片段代换系春恢356为材料,设置4个磷肥施用水平,分别为磷肥施用量0 kg/hm2(P0,不施磷)、常规施磷量的50%(P50)、常规施磷量的75%(P75)和常规施磷量(P100,P2O5施用量为75 kg/hm2),评价OsCERK1DY基因在籼稻中与丛枝菌根（AM）真菌的共生效应。结果表明,与春恢350相比,春恢356根系中AM真菌定殖率大幅提升；总体来说，在田间不同磷肥水平下其叶绿素含量、干物质量、磷吸收量和磷肥吸收利用率高于春恢350。2年田间试验结果显示,春恢356有效穗数在不同磷肥水平下都显著高于春恢350(P＜0.05),在P0、P50、P75 3个磷肥水平下春恢356实际产量显著高于春恢350(P＜0.05)。通过对水稻根际土壤微生物多样性分析,结果显示,与春恢350相比,春恢356根际土壤微生物具有更高的物种丰富度和多样性,富集了较多金黄杆菌属(Chryseobacterium)细菌和埃氏慢生根瘤菌属(Bradyrhizobium)细菌,碳氢化合物降解功能基因和固氮作用功能基因相对丰度显著(P＜0.05)或极显著(P＜0.01)增加。综上所述,将OsCERK1DY基因导入籼稻可以产生有益的共生效应,可用于培育养分高效利用的水稻品种。
Abstract:
In this study, using the indica rice restorer line Chunhui 350 as the control and the single-segment substitution line Chunhui 356, which carries the arbuscular mycorrhizal (AM) fungus high-efficiency symbiosis gene OsCERK1DY, as the experimental material, four phosphorus (P) fertilizer application levels were set: 0 kg/hm2 (P0), 50% of the conventional phosphorus application rate (P50), 75% of the conventional phosphorus application rate (P75), and the conventional phosphorus application rate (P100, with P2O5 application at 75 kg/hm2). The symbiotic effect of the OsCERK1DY gene with arbuscular mycorrhizal (AM) fungi in indica rice was evaluated. The results showed that, compared with Chunhui 350, the AM fungal colonization rate in the roots of Chunhui 356 was significantly increased. Overall, under different phosphorus fertilizer levels in the field, its chlorophyll content, dry matter weight, phosphorus uptake, and phosphorus fertilizer use efficiency were higher than those of Chunhui 350. Results of the two-year field experiment showed that the effective panicle number of Chunhui 356 was significantly higher than that of Chunhui 350 under all phosphorus fertilizer levels (P<0.05). Under the three treatments P0, P50, and P75, the actual yield of Chunhui 356 was significantly higher than that of Chunhui 350 (P<0.05). Analysis of the rice rhizosphere soil microbial diversity showed that, compared with Chunhui 350, the rhizosphere soil of Chunhui 356 exhibited higher species richness and diversity, with enrichment of more bacteria from the genera Chryseobacterium and Bradyrhizobium. The relative abundances of functional genes related to hydrocarbon degradation and nitrogen fixation were significantly (P<0.05) or extremely significantly (P<0.01) increased. In conclusion, introducing the OsCERK1DY gene into indica rice can produce beneficial symbiotic effects and can be used to breed rice cultivars with high nutrient use efficiency.

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备注/Memo

备注/Memo:
收稿日期：2025-05-29基金项目：江西省农业科学院基础研究与人才培养专项(JXSNKYJCRC202316)；国家红壤改良工程技术研究中心开放基金课题(2020NETRCRSI-7)；江西省主要学科学术和技术带头人培养项目(20232BCJ22014)作者简介：杨宙(1981-),男,湖北荆门人，博士，副研究员，主要从事水稻遗传育种研究。（E-mail）yangzhou0822@163.com通讯作者：黄仁良,(E-mail)huang_rls@126.com
更新日期/Last Update: 2026-05-11