[1]苏珹怡,伍家辉,王佩云,等.外源褪黑素对淹水胁迫下竹叶花椒的生理调控效应[J].江苏农业学报,2026,42(03):616-624.[doi:doi:10.3969/j.issn.1000-4440.2026.03.020]
　SU Chengyi,WU Jiahui,WANG Peiyun,et al.Effects of melatonin on physiological and biochemical characteristics of Zanthoxylum armatum under waterlogging stress[J].,2026,42(03):616-624.[doi:doi:10.3969/j.issn.1000-4440.2026.03.020]
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外源褪黑素对淹水胁迫下竹叶花椒的生理调控效应()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年03期
页码:
616-624
栏目:
园艺
出版日期:
2026-03-31

文章信息/Info

Title:
Effects of melatonin on physiological and biochemical characteristics of Zanthoxylum armatum under waterlogging stress
作者:
苏珹怡1; 2; 伍家辉1; 2; 王佩云1; 3; 吴焦焦1; 4; 秦中维5; 惠文凯1; 龚伟1; 王景燕1
(1.四川农业大学长江上游森林生态与保育四川省重点实验室,四川成都611130；2.中铁二十三局集团有限公司,四川成都610072；3.四川省林业工作总站,四川成都610081；4.成都工业学院材料与环境工程学院,四川成都611730；5.达川区大树镇农业综合服务中心,四川达州635743)
Author(s):
SU Chengyi1; 2; WU Jiahui1; 2; WANG Peiyun1; 3; WU Jiaojiao1; 4; QIN Zhongwei5; HUI Wenkai1; GONG Wei1; WANG Jingyan1
(1.Forest Ecology and Conservation in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China;2.China Railway 23rd Bureau Group Corporation Limited, Chengdu 610072, China;3.Sichuan Provincial General Forestry Station, Chengdu 610081, China;4.School of Materials and Environmental Engineering, Chengdu Technological University, Chengdu 611730, China;5.Agricultural Comprehensive Service Center of Dashu Town, Dachuan District, Dazhou 635743, China)
关键词:
竹叶花椒; 褪黑素; 淹水胁迫; 氧化损伤
Keywords:
Zanthoxylum armatum; melatonin; waterlogging stress; oxidative damage
分类号:
S573
DOI:
doi:10.3969/j.issn.1000-4440.2026.03.020
文献标志码:
A
摘要:
本研究通过外源施加不同浓度褪黑素,分析其对淹水胁迫下竹叶花椒生理的调控作用以确定最佳施用浓度,试验采用盆栽方式,以竹叶花椒幼苗为材料,设置不淹水对照(CK)及淹水 1 d、2 d、3 d、6 d共4个淹水胁迫处理,同时针对各淹水处理设置0 μmol/L、400 μmol/L、800 μmol/L 3个浓度褪黑素进行外源灌根,探究不同淹水时长与褪黑素浓度组合对竹叶花椒形态特征及生理生化指标的影响。结果显示,表型上淹水 2 d 以上时竹叶花椒植株地上部与根系均出现明显涝害症状,经褪黑素处理后损伤减轻，且400 μmol/L褪黑素缓解效果最优；生理指标方面,与CK相比,淹水胁迫下竹叶花椒根系中呼吸酶乙醇脱氢酶(ADH)、丙酮酸脱羧酶(PDC)活性随淹水胁迫时间延长呈上升趋势；叶片中过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)活性随淹水时间延长呈先上升后下降趋势,超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、相对电导率、脯氨酸(PRO)含量随淹水时间延长持续上升,叶片相对含水量则持续下降；调控效应上,400 μmol/L和800 μmol/L 褪黑素均能有效调控上述生理指标,其中400 μmol/L 褪黑素不仅可以显著降低ADH活性、PDC活性、相对电导率、MDA含量,还能显著提升SOD、POD、CAT、APX活性及叶片相对含水量、PRO含量。综上,400 μmol/L是本试验中调控竹叶花椒耐涝性的最佳褪黑素浓度,其通过调节幼苗呼吸代谢相关酶活性、平衡细胞内氧化还原状态、稳定渗透调节系统等生理代谢途径,协同增强竹叶花椒幼苗的耐涝能力。
Abstract:
To determine the optimal concentration of melatonin, this study analyzed its regulatory effect on the physiological indicators of Zanthoxylum armatum under waterlogging stress by exogenously applying different concentrations of melatonin. The experiment was conducted using a potted method, with Zanthoxylum armatum seedlings as the test material. A non-waterlogging control group (CK) and four waterlogging stress treatments (one day, two days, three days, and six days of waterlogging) were set up. Meanwhile, for each waterlogging treatment, three melatonin concentrations (0 μmol/L, 400 μmol/L, and 800 μmol/L) were applied through exogenous root irrigation to explore the effects of different combinations of waterlogging duration and melatonin concentration on the morphological characteristics, physiological and biochemical indicators of Zanthoxylum armatum. The results showed that in terms of phenotype, when waterlogging lasted for more than two days, obvious waterlogging damage symptoms appeared in both the aboveground parts and root systems of Zanthoxylum armatum plants; after melatonin treatment, the damage was alleviated, and the 400 μmol/L melatonin treatment showed the best mitigation effect. In terms of physiological indicators, compared with CK, under waterlogging stress, the activities of alcohol dehydrogenase (ADH) and pyruvate decarboxylase (PDC) in the roots of Zanthoxylum armatum showed an increasing trend, and the activities of peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) in the leaves of Zanthoxylum armatum first increased and then decreased with the extension of waterlogging time. Meanwhile, the superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, relative electrical conductivity, and proline (PRO) content increased continuously with the prolongation of waterlogging time, while the relative water content of leaves decreased continuously. In terms of regulatory effects, both 400 μmol/L and 800 μmol/L melatonin could effectively regulate the above-mentioned physiological indicators. Among them, 400 μmol/L melatonin not only significantly reduced the ADH activity, PDC activity, relative electrical conductivity, and MDA content, but also significantly increased the activities of SOD, POD, CAT, and APX, as well as the relative water content of leaves and PRO content. In conclusion, 400 μmol/L is the optimal melatonin concentration for regulating the waterlogging tolerance of Zanthoxylum armatum in this experiment. It synergistically enhances the waterlogging tolerance of Zanthoxylum armatum seedlings by regulating physiological metabolic pathways, including modulating respiratory enzyme activities, balancing the intracellular redox status, and stabilizing the osmotic adjustment system.

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备注/Memo

备注/Memo:
收稿日期:2025-03-31基金项目:四川省科技厅育种攻关项目(2021YFYZ0032、2016NYZ0035)；国家重点研发项目(2020YFD1000700、 2018YFD1000605)；四川省花椒创新团队项目(sccxtd-2024-23)作者简介:苏珹怡(1997-),女,四川万源人,硕士,助理工程师,主要从事经济林培育研究。(E-mail)904525759@qq.com通讯作者:王景燕,(E-mail)wangjingyan@sicau.edu.cn
更新日期/Last Update: 2026-04-17