[1]赵坤,周聪,陈汉,等.稻田土壤N2O还原潜力的影响因素[J].江苏农业学报,2026,42(03):563-573.[doi:doi:10.3969/j.issn.1000-4440.2026.03.014]
　ZHAO Kun,ZHOU Cong,CHEN Han,et al.Influencing factors of N2O reduction potential in paddy soils[J].,2026,42(03):563-573.[doi:doi:10.3969/j.issn.1000-4440.2026.03.014]
点击复制

稻田土壤N2O还原潜力的影响因素()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年03期
页码:
563-573
栏目:
耕作栽培·资源环境
出版日期:
2026-03-31

文章信息/Info

Title:
Influencing factors of N2O reduction potential in paddy soils
作者:
赵坤; 周聪; 陈汉; 滕钊军; 张耀鸿
(南京信息工程大学生态与应用气象学院,江苏南京210044)
Author(s):
ZHAO Kun; ZHOU Cong; CHEN Han; TENG Zhaojun; ZHANG Yaohong
(School of Ecology and Applied Meteorology, Nanjing University of Information Science and Technology, Nanjing 210044, China)
关键词:
稻田土壤; N2O还原; 可溶性有机碳; nosZ基因
Keywords:
paddy soil; N2O reduction; soluble organic carbon; nosZ gene
分类号:
S154.1
DOI:
doi:10.3969/j.issn.1000-4440.2026.03.014
文献标志码:
A
摘要:
稻田是陆地生态系统N2O的主要排放源之一,明确土壤N2O还原潜力对稻田N2O减排和缓解大气温室效应具有重要意义。本研究选取江苏常熟、黑龙江建三江、江西鹰潭、四川资阳、海南三亚和贵州贵阳6个典型稻田土壤进行厌氧培养,分析稻田土壤N2O还原速率和N2O还原酶基因nosZ I和nosZ II拷贝数的差异性及其关键影响因素。结果表明,供试稻田土壤的可溶性有机碳(DOC)含量为9.52～61.21 mg/kg,其变异系数高于土壤总有机碳含量。不同地区稻田土壤N2O还原速率存在显著差异,其中,常熟稻田土壤的N2O平均还原速率为28.55 μg/(g·d),是鹰潭稻田土壤的5.9倍。稻田土壤N2O还原速率与土壤DOC含量呈显著正相关。 6个地区稻田土壤N2O还原酶基因nosZ I和nosZ II的拷贝数分别为0.90×107～9.09×107 copies/g和1.01×107～6.53×107 copies/g。nosZ I基因拷贝数与稻田土壤N2O还原速率呈显著正相关,而nosZ II拷贝数与N2O还原速率的相关性不显著。稻田反硝化过程的N2O净排放速率与N2O还原速率呈显著正相关。上述结果说明,土壤活性碳含量是影响稻田N2O还原过程的关键因素,供试稻田土壤的N2O还原过程主要由nosZ I型微生物驱动。
Abstract:
The ecosystem of paddy fields is a primary contributor to N2O emissions within agricultural land, and studying the N2O reduction potential in paddy fields is of great significance for reducing N2O emissions and mitigating the atmospheric greenhouse effect. In this experiment, six typical paddy field soils from Jiangsu Changshu, Helongjiang Jiansanjiang, Jiangxi Yingtan, Sichuan Ziyang, Hainan Sanya and Guizhou Guiyang were selected for anaerobic cultivation to study the differences in N2O reduction rates and copy numbers of N2O reductase genes nosZ I and nosZ II in the plough layer of paddy fields, as well as their key influencing factors. The results showed that dissolved organic carbon (DOC) content in the tested paddy soils ranged from 9.52 mg/kg to 61.21 mg/kg, which had a higher coefficient of variation than the total organic carbon content. The soil N2O reduction rate in paddy fields showed significant regional variation, and the averaged N2O reduction rate in Changshu paddy fields was 28.55 μg/(g·d), which was 5.9 times higher than that in the Yingtan paddy fields. Correlation analysis found that there was a significant positive correlation between the N2O reduction rate in paddy fields and soil DOC content. Quantitative PCR results showed that the copy numbers of N2O reductase genes nosZ I and nosZ II in the six paddy soils were 0.90×107-9.09×107copies/g and 1.01×107-6.53×107copies/g, respectively. The copy number of gene nosZ I was significantly and positively correlated with the N2O reduction rate in paddy fields, while the copy number of gene nosZ II did not reach a significant level. Moreover, N2O net emission rate during the denitrification process was significantly positively correlated with the N2O reduction rate in paddy fields. This results suggested that the active carbon content was an important factor in the process of N2O reduction in paddy fields, and N2O reduction process in the tested paddy fields might be mainly driven by nosZ I type microorganisms.

参考文献/References:

[1]SU J, HU C, YAN X, et al. Expression of barley SUSIBA2 transcription factor yields high-starch low-methane rice[J]. Nature,2015,523(7562):602-606.
[2]赵光影,刘景双,王洋. 陆地生态系统N2O排放源研究进展[J]. 土壤通报,2008,39(5):1192-1197.
[3]张玉铭,胡春胜,张佳宝,等. 农田土壤主要温室气体(CO2、CH4、N2O)的源/汇强度及其温室效应研究进展[J]. 中国生态农业学报,2011,19(4):966-975.
[4]REAY D S, DAVIDSON E A, SMITH K A, et al. Global agriculture and nitrous oxide emissions[J]. Nature Climate Change,2012,2:410-416.
[5]SMITH K A, MOSIER A R, CRUTZEN P J, et al. The role of N2O derived from crop-based biofuels,and from agriculture in general,in Earth’s climate[J]. Philosophical Transactions of the Royal Society of London. Series B,Biological Sciences,2012,367(1593):1169-1174.
[6]MONTZKA S A, DLUGOKENCKY E J, BUTLER J H. Non-CO2 greenhouse gases and climate change[J]. Nature,2011,476(7358):43-50.
[7]宋文质,王少彬,苏维瀚,等. 我国农田土壤的主要温室气体CO2、CH4和N2O排放研究[J]. 环境科学,1996,17(1):85-88.
[8]INTERGOVERNMENTAL PANEL ON CLIMATE CHANGE. Climate change 2007:mitigation contribution of working group Ⅲ to the fourth assessment report of the intergovernmental panel on climate change[M]. Cambridge:Cambridge University Press,2007.
[9]王晓洁,卑其成,刘钢,等. 不同类型水稻土微生物群落结构特征及其影响因素[J]. 土壤学报,2021,58(3):767-776.
[10]KIM G W, KIM P J, KHAN M I, et al. Effect of rice planting on nitrous oxide (N2O) emission under different levels of nitrogen fertilization[J]. Agronomy,2021,11(2):217.
[11]马二登,马静,徐华,等. 施肥对稻田N2O排放的影响[J]. 农业环境科学学报,2009,28(12):2453-2458.
[12]邹建文,黄耀,宗良纲,等. 稻田CO2、CH4和N2O排放及其影响因素[J]. 环境科学学报,2003,23(6):758-764.
[13]SMITH C J, PATRICK W H. Nitrous oxide emission as affected by alternate anaerobic and aerobic conditions from soil suspensions enriched with ammonium sulfate[J]. Soil Biology and Biochemistry,1983,15(6):693-697.
[14]李冀,朱莹,张晓君. 非典型氧化亚氮还原酶基因nosZ II研究进展[J]. 微生物学通报,2017,44(7):1714-1719.
[15]SANFORD R A, WAGNER D D, WU Q Z, et al. Unexpected nondenitrifier nitrous oxide reductase gene diversity and abundance in soils[J]. Proceedings of the National Academy of Sciences of the United States of America,2012,109(48):19709-19714.
[16]JONES C M, GRAF D R H, BRU D, et al. The unaccounted yet abundant nitrous oxide-reducing microbial community:a potential nitrous oxide sink[J]. The ISME Journal,2013,7(2):417-426.
[17]JONES C M, SPOR A, BRENNAN F P, et al. Recently identified microbial guild mediates soil N2O sink capacity[J]. Nature Climate Change,2014,4:801-805.
[18]宋雅琦,王玲,李昆,等. 土壤微生物还原N2O机制及其研究进展[J]. 环境科学与技术,2019,42(9):82-90.
[19]ZUMFT W G, KRNER H. Nitrous oxide reductases[M]//BOTHE H, FERGUSON S J, NEWTON W E. Biology of the Nitrogen Cycle. Amsterdam:Elsevier,2007:67-81.
[20]鲍士旦. 土壤农化分析[M]. 3版. 北京:中国农业出版社,2000.
[21]郑燕,侯海军,秦红灵,等. 施氮对水稻土N2O释放及反硝化功能基因(narG/nosZ)丰度的影响[J]. 生态学报,2012,32(11):3386-3393.
[22]饶旭东,谢晴,薛梦琪,等. 外源氮对滨海围垦稻田土壤N2O还原速率的影响机制研究[J]. 中国土壤与肥料,2022(8):76-83.
[23]MINAMI K. Atmospheric methane and nitrous oxide:sources,sinks and strategies for reducing agricultural emissions[J]. Nutrient Cycling in Agroecosystems,1997,49(1):203-211.
[24]MATHIEU O, LVQUE J, HNAULT C, et al. Emissions and spatial variability of N2O,N2 and nitrous oxide mole fraction at the field scale,revealed with 15N isotopic techniques[J]. Soil Biology and Biochemistry,2006,38(5):941-951.
[25]汪方圆,张耀鸿,饶旭东,等. 围垦对滨海稻田土壤N2O还原潜力的影响[J]. 农业环境科学学报,2020,39(11):2668-2674.
[26]WANG X M, WU M, WEI Z J, et al. Investigating drivers of free-living diazotroph activity in paddy soils across China[J]. Soil Biology and Biochemistry,2024,199:109601.
[27]WANG X M, ZHANG Y M, ZHOU H, et al. Investigating drivers of N2 loss and N2O reducers in paddy soils across China[J]. Science of the Total Environment,2024,954:176287.
[28]QIN H L, WANG D, XING X Y, et al. A few key nirK- and nosZ-denitrifier taxa play a dominant role in moisture-enhanced N2O emissions in acidic paddy soil[J]. Geoderma,2021,385:114917.
[29]XING X Y, TANG Y F, XU H F, et al. Warming shapes nirS- and nosZ-type denitrifier communities and stimulates N2O emission in acidic paddy soil[J]. Applied and Environmental Microbiology,2021,87(12):e0296520.
[30]YAN D Z, WANG D J, YANG L Z. Long-term effect of chemical fertilizer,straw,and manure on labile organic matter fractions in a paddy soil[J]. Biology and Fertility of Soils,2007,44(1):93-101.
[31]李彬彬,马军花,武兰芳. 土壤溶解性有机物对CO2和N2O排放的影响[J]. 生态学报,2014,34(16):4690-4697.
[32]王玲,邢肖毅,秦红灵,等. 淹水水稻土消耗N2O能力及机制[J]. 环境科学,2017,38(4):1633-1639.
[33]徐鹏,邬磊,胡金丽,等. 添加葡萄糖、乙酸、草酸对红壤旱地土壤氮素矿化及反硝化的影响[J]. 环境科学学报,2017,37(12):4740-4746.
[34]CAVIGELLI M A, ROBERTSON G P. Role of denitrifier diversity in rates of nitrous oxide consumption in a terrestrial ecosystem[J]. Soil Biology and Biochemistry,2001,33(3):297-310.
[35]高焕平,刘世亮,赵颖,等. 秸秆与氮肥调节C/N比对潮土CH4,CO2和N2O排放/吸收的影响[J]. 土壤通报,2019,50(1):157-164.
[36]JENSEN E S. Nitrogen immobilization and mineralization during initial decomposition of 15N-labelled pea and barley residues[J]. Biology and Fertility of Soils,1997,24(1):39-44.
[37]吴梦琴,李成芳,盛锋,等. 基于DNDC模型评估湖北省不同稻作系统不同管理措施温室气体排放的周年变化[J]. 中国生态农业学报(中英文),2021,29(9):1480-1492.
[38]韩燕云,吴永红,李丹,等. 微生物介导的稻田水土界面温室气体排放及其农事减排措施研究进展[J]. 环境科学研究,2023,36(12):2369-2381.
[39]BARTH G, VON TUCHER S, SCHMIDHALTER U. Influence of soil parameters on the effect of 3,4-dimethylpyrazole-phosphate as a nitrification inhibitor[J]. Biology and Fertility of Soils,2001,34(2):98-102.
[40]XU X, BOECKX P, WANG Y, et al. Nitrous oxide and methane emissions during rice growth and through rice plants:effect of dicyandiamide and hydroquinone[J]. Biology and Fertility of Soils,2002,36(1):53-58.
[41]赵苗苗,张文忠,裴瑶,等. 农田温室气体N2O排放研究进展[J]. 作物杂志,2013(4):25-31.
[42]王丽丽,赵林,谭欣,等. 不同碳源及其碳氮比对反硝化过程的影响[J]. 环境保护科学,2004,30(1):15-18.
[43]刘春梅,魏文学,盛荣,等. 氧化亚氮还原酶基因nosZ II及与环境的关系研究进展[J]. 应用与环境生物学报,2018,24(3):651-656.
[44]DOMEIGNOZ-HORTA L A, SPOR A, BRU D, et al. The diversity of the N2O reducers matters for the N2O:N2 denitrification end-product ratio across an annual and a perennial cropping system[J]. Frontiers in Microbiology,2015,6:971.
[45]王玲,李昆,宋雅琦,等. 浅表层水稻土N2O消耗能力及其与N2O还原微生物的耦合关系[J]. 生态学报,2019,39(20):7602-7610.
[46]DINI-ANDREOTE F, BROSSI M J, VAN ELSAS J D, et al. Reconstructing the genetic potential of the microbially-mediated nitrogen cycle in a salt marsh ecosystem[J]. Frontiers in Microbiology,2016,7:902.
[47]PASCUAL M B, SNCHEZ-MONEDERO M , CAYUELA M L, et al. Biochar as electron donor for reduction of N2O by Paracoccus denitrificans[J]. FEMS Microbiology Ecology,2020,96(8):fiaa133.
[48]DONG W X, WALKIEWICZ A, BIEGANOWSKI A, et al. Biochar promotes the reduction of N2O to N2 and concurrently suppresses the production of N2O in calcareous soil[J]. Geoderma,2020,362:114091.

相似文献/References:

[1]谢晴,饶旭东,周聪,等.夜间增温条件下施硅稻田N2O还原对外源氮的响应[J].江苏农业学报,2022,38(01):87.[doi:doi:10.3969/j.issn.1000-4440.2022.01.010]
　XIE Qing,RAO Xu-dong,ZHOU Cong,et al.Response of N2O reduction to exogenous nitrogen in paddy field under night warming and silicon application[J].,2022,38(03):87.[doi:doi:10.3969/j.issn.1000-4440.2022.01.010]

备注/Memo

备注/Memo:
收稿日期：2025-06-08基金项目：国家自然科学基金面上项目(42175138、42377295)作者简介：赵坤(2001-),男,安徽滁州人,硕士研究生,主要研究方向为碳氮循环与全球变化。(E-mail)zk3102619152@163.com通讯作者：张耀鸿,(E-mail)yhzhang@nuist.edu.cn
更新日期/Last Update: 2026-04-17