[1]韩志彬,张春,史永军,等.向日葵HaOSCA1.2基因克隆及表达特性分析[J].江苏农业学报,2026,42(03):475-484.[doi:doi:10.3969/j.issn.1000-4440.2026.03.005]
　HAN Zhibin,ZHANG Chun,SHI Yongjun,et al.Cloning and expression characteristics of HaOSCA1.2 gene in sunflower[J].,2026,42(03):475-484.[doi:doi:10.3969/j.issn.1000-4440.2026.03.005]
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向日葵HaOSCA1.2基因克隆及表达特性分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年03期
页码:
475-484
栏目:
遗传育种·生理生化
出版日期:
2026-03-31

文章信息/Info

Title:
Cloning and expression characteristics of HaOSCA1.2 gene in sunflower
作者:
韩志彬1; 张春1; 史永军2; 安琪3; 彭荣杰1; 陈阳1
(1.河套学院农学系/河套绿色农畜产品生产现代产业学院,内蒙古巴彦淖尔015000；2.巴彦淖尔市农牧局,内蒙古巴彦淖尔015000；3.内蒙古自治区农畜产品产销服务中心,内蒙古呼和浩特010011)
Author(s):
HAN Zhibin1; ZHANG Chun1; SHI Yongjun2; AN Qi3; PENG Rongjie1; CHEN Yang1
(1.Department of Agronomy, Hetao College/Hetao Modern Industry Institute of Green Agricultural and Livestock Products Production, Bayannur 015000, China;2.Bureau of Agriculture and Animal Husbandry of Bayannur City, Bayannur 015000, China;3.Inner Mongolia Autonomous Region Service Center for Production and Marketing of Agricultural and Livestock Products, Hohhot 010011, China)
关键词:
向日葵; OSCA1.2基因; 表达特性; 非生物胁迫
Keywords:
sunflower; OSCA1.2 gene; expression characteristics; abiotic stress
分类号:
S565.5
DOI:
doi:10.3969/j.issn.1000-4440.2026.03.005
文献标志码:
A
摘要:
高渗透压门控钙通透性通道家族基因(OSCA)在植物非生物胁迫响应中发挥关键调控作用。为明确向日葵HaOSCA1.2基因的分子特征及其对逆境胁迫的响应机制,本研究克隆了该基因,其编码区(CDS)全长2 322 bp,编码773个氨基酸,编码的蛋白质相对分子量为88 430,理论等电点8.81,含RSN1_7TM保守域,为疏水性跨膜蛋白,二级结构以α-螺旋为主；进化分析结果明确,HaOSCA1.2蛋白与除虫菊、牛蒡、莴苣OSCA蛋白的氨基酸序列相似度较高(88.93%～89.13%),且亲缘关系较近。组织特异性表达分析结果显示,HaOSCA1.2基因在向日葵叶片中的表达量显著高于在根中和茎中的表达量(P＜0.05)；非生物胁迫响应分析发现,HaOSCA1.2基因对干旱胁迫、盐胁迫、混合盐碱胁迫均表现出快速上调表达特性；蛋白质互作预测结果显示,HaOSCA1.2蛋白与DnaJ热休克蛋白(HSP40)家族的2个成员存在潜在互作关系。本研究明确了向日葵HaOSCA1.2基因的分子特征及非生物胁迫响应规律,为解析向日葵抗逆分子机制及抗逆育种提供了理论依据与基因资源。
Abstract:
The hyperosmolarity-gated calcium-permeable channel family genes (OSCA) play a key regulatory role in plant responses to abiotic stresses. To clarify the molecular characteristics of sunflower HaOSCA1.2 and its response mechanism to stresses, this study cloned the HaOSCA1.2 gene. The coding sequence (CDS) of HaOSCA1.2 is 2 322 bp in length, encoding 773 amino acids. The relative molecular weight of the encoded protein is 88 430, with a theoretical isoelectric point of 8.81. It contains the RSN1_7TM conserved domain and is a hydrophobic transmembrane protein, whose secondary structure is dominated by α-helices. Evolutionary analysis revealed that HaOSCA1.2 shared high amino acid sequence similarity (88.93%-89.13%) with OSCA proteins from Tanacetum cinerariifolium, Arctium lappa, and Lactuca sativa, indicating a close phylogenetic relationship. Tissue-specific expression analysis revealed that the expression level of HaOSCA1.2 in sunflower leaves was significantly higher than that in roots and stems (P<0.05). Analysis of abiotic stress response showed that the HaOSCA1.2 gene exhibited rapid and upregulated expression under drought, high salt, and mixed saline-alkali stresses. Protein-protein interaction prediction indicated that HaOSCA1.2 potentially interacted with two members of the DnaJ heat shock protein (HSP40) family. This study clarifies the molecular characteristics and abiotic stress response patterns of the sunflower HaOSCA1.2 gene, providing a theoretical basis and genetic resources for analyzing the molecular mechanisms of sunflower stress resistance and for breeding stress-resistant varieties.

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备注/Memo

备注/Memo:
收稿日期：2025-09-21基金项目：内蒙古自治区科技创新重大示范项目(2025ZDSF0014)；河套学院高层次人才启动项目(HYRC202319)；河套学院科研科技创新团队建设项目(HTKCT-A202405)作者简介：韩志彬(2004-),女,山西大同人,本科,主要从事作物抗逆生理及分子生物学研究。(E-mail)hanzhibin4738@163.com通讯作者：陈阳,(E-mail)chenyangrz@126.com
更新日期/Last Update: 2026-04-17