[1]郭炜,张巧凤,祝秀亮,等.转抗菌肽基因Ace-AMP-sn抗纹枯病小麦新品系的创制与评价[J].江苏农业学报,2026,42(03):446-451.[doi:doi:10.3969/j.issn.1000-4440.2026.03.002]
　GUO Wei,ZHANG Qiaofeng,ZHU Xiuliang,et al.Creation and evaluation of a new wheat line transformed with antimicrobial peptide gene Ace-AMP-sn resistant to sharp eyespot[J].,2026,42(03):446-451.[doi:doi:10.3969/j.issn.1000-4440.2026.03.002]
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转抗菌肽基因Ace-AMP-sn抗纹枯病小麦新品系的创制与评价()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年03期
页码:
446-451
栏目:
遗传育种·生理生化
出版日期:
2026-03-31

文章信息/Info

Title:
Creation and evaluation of a new wheat line transformed with antimicrobial peptide gene Ace-AMP-sn resistant to sharp eyespot
作者:
郭炜1; 张巧凤1; 祝秀亮2; 付必胜1; 蔡瑾1; 刘颖1; 翟文玲1; 吴小有1; 蔡士宾1; 张增艳2; 吴纪中1
(1.江苏省农业科学院种质资源与生物技术研究所,江苏南京210014；2.中国农业科学院作物科学研究所/作物基因资源与育种全国重点实验室,北京100081)
Author(s):
GUO Wei1; ZHANG Qiaofeng1; ZHU Xiuliang2; FU Bisheng1; CAI Jin1; LIU Ying1; ZHAI Wenling1; WU Xiaoyou1; CAI Shibin1; ZHANG Zengyan2; WU Jizhong1
(1.Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2.Institute of Crop Sciences/National Key Laboratory of Crop Gene Resources and Breeding, Chinese Academy of Agricultural Sciences, Beijing 100081, China)
关键词:
小麦纹枯病; 抗菌肽; 转基因; 抗性
Keywords:
wheat sharp eyespot; antimicrobial peptide; transgene; resistance
分类号:
S512.1
DOI:
doi:10.3969/j.issn.1000-4440.2026.03.002
文献标志码:
A
摘要:
纹枯病是近年来威胁中国小麦生产的主要土传真菌性病害,培育抗病品种是防控该病害最为经济有效的途径。本研究以T2代转洋葱抗菌肽基因Ace-AMP-sn的扬麦18为材料,通过连续多代抗性鉴定,得到3个T7代转基因株系E308、E328、E332。实时荧光定量PCR（qRT-PCR）结果显示,T7代转基因株系E308、E328和E332中Ace-AMP-sn基因的相对表达量均显著高于受体扬麦18(P＜0.05)。抗病鉴定结果表明,3个T7代转基因株系的纹枯病病情指数均显著低于扬麦18(P＜0.05),与抗病品种CI12633的纹枯病病情指数无显著差异(P＞0.05),表明Ace-AMP-sn基因的导入可有效提高小麦对纹枯病的抗性。所有T7代转基因株系的抽穗期、株高、穗部性状及产量等关键农艺性状与扬麦18均无显著差异(P＞0.05)，表明该基因的导入未对主要农艺性状产生不利影响。本研究为小麦抗纹枯病遗传改良提供了抗性突出、农艺性状优良的新种质。
Abstract:
Sharp eyespot has become a major soil-borne fungal disease threatening wheat production in China in recent years. Breeding disease-resistant varieties is the most economical and effective strategy for controlling this disease. In this study, the T2 generation wheat variety Yangmai 18 transformed with the onion antimicrobial peptide gene Ace-AMP-sn was used as the material. Through successive generations of resistance identification, three T7 transgenic lines E308, E328, and E332 were obtained. Quantitative real-time PCR (qRT-PCR) results showed that the relative expression levels of Ace-AMP-sn in T7 transgenic lines E308, E328, and E332 were significantly higher than those in the recipient variety Yangmai 18 (P＜0.05). Results of disease resistance identification demonstrated that the sharp eyespot disease indices of the three T7 transgenic lines were significantly lower than that of Yangmai 18 (P＜0.05), while no significant difference was observed compared with the resistant variety CI12633 (P＞0.05). These results indicated that the introduction of Ace-AMP-sn could effectively improve resistance to sharp eyespot in wheat. Furthermore, no significant differences (P＞0.05) were detected between all T7 transgenic lines and Yangmai 18 in key agronomic traits, including heading date, plant height, spike traits, and yield components, suggesting that the introduction of this gene had no adverse effects on main agronomic traits. This study provides new germplasm with outstanding resistance and excellent agronomic traits for the genetic improvement of sharp eyespot resistance in wheat.

参考文献/References:

[1]REN Y, YU P B, WANG Y, et al. Development of a rapid approach for detecting sharp eyespot resistance in seedling-stage wheat and its application in Chinese wheat cultivars[J]. Plant Disease,2020,104(6):1662-1667.
[2]LIU C Y, GUO W, ZHANG Q F, et al. Genetic dissection of adult plant resistance to sharp eyespot using an updated genetic map of Niavt14× Xuzhou25 winter wheat recombinant inbred line population[J]. Plant Disease,2021,105(4):997-1005.
[3]TOMIOKA K, SEKIGUCHI H, BAN Y, et al. Sharp eyespot of barley, bread wheat and durum wheat caused by Ceratobasidium cereale in Japan[J]. Journal of General Plant Pathology,2021,87(2):110-112.
[4]WANG K, RONG W, LIU Y P, et al. Wheat elongator subunit 4 is required for epigenetic regulation of host immune response to Rhizoctonia cerealis[J]. The Crop Journal,2020,8(4):565-576.
[5]周淼平,姚金保,张鹏,等. 小麦幼苗纹枯病抗性评价新方法[J]. 江苏农业学报,2017,33(1):61-66.
[6]王金凤,杜丽璞,李钊,等. 抗纹枯病、根腐病的转SN1基因小麦的获得与鉴定[J]. 作物学报,2012,38(5):773-779.
[7]齐永霞,陈莉,丁克坚. 小麦纹枯病田间药剂防治技术研究[J]. 麦类作物学报,2015,35(4):577-583.
[8]WU X J, CHENG K, ZHAO R H, et al. Quantitative trait loci responsible for sharp eyespot resistance in common wheat CI12633[J]. Scientific Reports,2017,7:11799.
[9]马成程,卢霖,号宇然,等. 小麦抗纹枯病分子基础研究进展[J].生物工程学报,2025,41(11):4443-4456.
[10]刘颖,张巧凤,付必胜,等. 小麦纹枯病抗源的遗传多样性及抗性基因位点SSR标记分析[J]. 作物学报,2015,41(11):1671-1681.
[11]刁慧珊,梁邦平,李家创,等. 156份小麦种质资源的纹枯病抗性鉴定与评价[J]. 麦类作物学报,2018,38(11):1381-1389.
[12]何彦江,刘彩云,翟文玲,等. 427份我国小麦种质资源的纹枯病抗性评价[J]. 江苏农业科学,2025,53(6):130-136.
[13]张会云,冯国华,刘东涛,等. 小麦种质资源对纹枯病的抗性鉴定及利用[J]. 西北农业学报,2009,18(1):213-216.
[14]李洪杰,王晓鸣,陈怀谷,等. 小麦-偃麦草杂种后代及小麦种质资源对纹枯病的抗性[J]. 作物学报,2013,39(6):999-1012.
[15]万映秀,王文相,张平治,等. 小麦纹枯病抗性鉴定技术及抗性资源筛选[J]. 中国农学通报,2009,25(7):223-226.
[16]蒋彦婕,吴纪中,蔡士宾,等. 小麦抗纹枯病种质资源的鉴定与评价[J]. 麦类作物学报,2013,33(3):589-594.
[17]高梦娟,赵贺莹,陈家辉,等. 小麦抗纹枯病新位点Qse.hnau-5AS的定位及其候选基因鉴定[J]. 作物学报,2025,51(8):2240-2250.
[18]于美,唐华丽,叶兴国. 利用转基因技术和基因编辑技术改良小麦进展[J]. 植物遗传资源学报,2023,24(1):102-116.
[19]CHEN L, ZHANG Z Y, LIANG H X, et al. Overexpression of TiERF1 enhances resistance to sharp eyespot in transgenic wheat[J]. Journal of Experimental Botany,2008,59(15):4195-4204.
[20]ZHU X L, QI L, LIU X, et al. The wheat ethylene response factor transcription factor pathogen-induced ERF1 mediates host responses to both the necrotrophic pathogen Rhizoctonia cerealis and freezing stresses[J]. Plant Physiology,2014,164(3):1499-1514.
[21]LIU X, ZHU X L, WEI X N, et al. The wheat LLM-domain-containing transcription factor TaGATA1 positively modulates host immune response to Rhizoctonia cerealis[J]. Journal of Experimental Botany,2020,71(1):344-355.
[22]ZHU X L, YANG K, WEI X N, et al. The wheat AGC kinase TaAGC1 is a positive contributor to host resistance to the necrotrophic pathogen Rhizoctonia cerealis[J]. Journal of Experimental Botany,2015,66(21):6591-6603.
[23]WEI X N, SHEN F D, HONG Y T, et al. The wheat calcium-dependent protein kinase TaCPK7-D positively regulates host resistance to sharp eyespot disease[J]. Molecular Plant Pathology,2016,17(8):1252-1264.
[24]ZHU X L, LU C G, DU L P, et al. The wheat NB-LRR gene TaRCR1 is required for host defence response to the necrotrophic fungal pathogen Rhizoctonia cerealis[J]. Plant Biotechnology Journal,2017,15(6):674-687.
[25]RONG W, LUO M Y, SHAN T L, et al. A wheat cinnamyl alcohol dehydrogenase TaCAD12 contributes to host resistance to the sharp eyespot disease[J]. Frontiers in Plant Science,2016,7:1723.
[26]WANG M X, ZHU X L, WANG K, et al. A wheat caffeic acid 3-O-methyltransferase TaCOMT-3D positively contributes to both resistance to sharp eyespot disease and stem mechanical strength[J]. Scientific Reports,2018,8:6543.
[27]ZHU X L, RONG W, WANG K, et al. Overexpression of TaSTT3b-2B improves resistance to sharp eyespot and increases grain weight in wheat[J]. Plant Biotechnology Journal,2022,20(4):777-793.
[28]周晓馥,苗璐,高峰,等. 利用生物信息学对植物抗菌肽的预测与分析[J]. 生物技术,2014,24(3):91-95.
[29]NAWROT R, BARYLSKI J, NOWICKI G, et al. Plant antimicrobial peptides[J]. Folia Microbiologica,2014,59(3):181-196.
[30]IWAI T, KAKU H, HONKURA R, et al. Enhanced resistance to seed-transmitted bacterial diseases in transgenic rice plants overproducing an oat cell-wall-bound thionin[J]. Molecular Plant-Microbe Interactions,2002,15(6):515-521.
[31]ALMASIA N I, BAZZINI A A, HOPP H E, et al. Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants[J]. Molecular Plant Pathology,2008,9(3):329-338.
[32]杨学智,徐伟豪,韩云哲,等. 水稻促生菌研究进展[J]. 江苏农业科学,2025,53(13):24-31.
[33]任燕,王小慧,肖阳,等. 益生菌添食对家蚕幼虫生理及蚕蛹养分特征的影响[J]. 南方农业学报,2025,56(4):1315-1325.
[34]魏盼琪,杨佳,郑玉才,等. 饲料中添加酶制剂和抗菌肽对藏鸡生产性能、抗氧化能力及免疫性能的影响[J]. 江苏农业学报,2024,40(10):1882-1890.
[35]刘姝瑶,李倩倩,文静,等. 克氏原螯虾duox1基因抵御金黄色葡萄球菌侵染的先天免疫机制[J]. 南方农业学报,2024,55(8):2485-2494.
[36]朱桃,张双玉,张路阳,等. 枯草芽孢杆菌4种环脂肽抗猪δ冠状病毒的研究[J]. 江苏农业科学,2024,52(4):182-186,192.
[37]ROY-BARMAN S, SAUTTER C, CHATTOO B B. Expression of the lipid transfer protein Ace-AMP1 in transgenic wheat enhances antifungal activity and defense responses[J]. Transgenic Research,2006,15(4):435-446.
[38]杨坤,刘欣,杜丽璞,等. 转AcAMP-sn基因抗全蚀病小麦新种质的创制与鉴定[J]. 作物学报,2014,40(1):22-28.
[39]YU G T, HATTA A, PERIYANNAN S, et al. Isolation of wheat genomic DNA for gene mapping and cloning[M]//PERIYANNAN S. Wheat Rust Diseases:Methods in Molecular Biology. New York:Humana Press,2017.
[40]LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-△△CT method[J]. Methods,2001,25(4):402-408.
[41]JIANG Y J, ZHU F F, CAI S B, et al. Quantitative trait loci for resistance to Sharp Eyespot (Rhizoctonia cerealis) in recombinant inbred wheat lines from the cross Niavt 14 × Xuzhou 25[J]. Czech Journal of Genetics and Plant Breeding,2016,52(4):139-144.
[42]LIU C Y, GUO W, WANG Y, et al. Introgression of sharp eyespot resistance from Dasypyrum villosum chromosome 2VL into bread wheat[J]. The Crop Journal,2023,11(5):1512-1520.
[43]PATKAR R N, CHATTOO B B. Transgenic indica rice expressing ns-LTP-like protein shows enhanced resistance to both fungal and bacterial pathogens[J]. Molecular Breeding,2006,17(2):159-171.

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备注/Memo

备注/Memo:
收稿日期:2025-11-07基金项目:转基因生物新品种培育重大专项（2016ZX08002-001）；江苏省农业科技自主创新基金项目[CX(23)3092]作者简介:郭炜（1989-），男，山西长治人，博士，助理研究员，主要从事小麦抗病种质资源挖掘与利用研究。（E-mail）guowei@jaas.ac.cn通讯作者:吴纪中，（E-mail）wujiz@jaas.ac.cn；张增艳，（E-mail）zhangzengyan@caas.cn
更新日期/Last Update: 2026-04-17