[1]李康宁,徐凌璐,宣恒鑫,等.水稻萌发期温度胁迫响应的转录调控机制[J].江苏农业学报,2026,42(03):433-445.[doi:doi:10.3969/j.issn.1000-4440.2026.03.001]
　LI Kangning,XU Linglu,XUAN Hengxin,et al.Transcriptional regulation mechanism of temperature stress response during rice germination stage[J].,2026,42(03):433-445.[doi:doi:10.3969/j.issn.1000-4440.2026.03.001]
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水稻萌发期温度胁迫响应的转录调控机制()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年03期
页码:
433-445
栏目:
遗传育种·生理生化
出版日期:
2026-03-31

文章信息/Info

Title:
Transcriptional regulation mechanism of temperature stress response during rice germination stage
作者:
李康宁1; 2; 徐凌璐2; 宣恒鑫1; 李程3; 高梦涛2
(1.江苏农林职业技术学院农学园艺学院,江苏句容212400；2.南京农业大学资源与环境科学学院/作物遗传与种质创新国家重点实验室,江苏南京211800；3.江苏省农业科学院粮食作物研究所,江苏南京210014)
Author(s):
LI Kangning1; 2; XU Linglu2; XUAN Hengxin1; LI Cheng3; GAO Mengtao2
(1.School of Agronomy and Horticulture, Jiangsu Vocational College of Agriculture and Forestry, Jurong 212400, China; 2.College of Resources and Environmental Sciences/State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 211800, China;3.Food Crop Research Institute, Jiangsu Academy of Agricultural Sciences， Nanjing 210014, China)
关键词:
水稻; 萌发; 温度胁迫; 转录组分析; 差异表达基因
Keywords:
rice; germination; temperature stress; transcriptome analysis; differentially expressed gene
分类号:
S511
DOI:
doi:10.3969/j.issn.1000-4440.2026.03.001
文献标志码:
A
摘要:
温度是影响水稻种子萌发和产量形成的重要因素。本研究以日本晴为供试材料,进行常温(NT,25 ℃)、低温(LT,15 ℃)和高温(HT,37 ℃)3个处理的暗培养萌发试验,并采集培养60 h的水稻种子开展转录组分析。结果表明,与常温处理相比,低温处理能显著延缓种子萌发,而高温处理则加速种子萌发。在低温和常温比较组、常温和高温比较组及低温和高温比较组中分别鉴定出8 010个、3 929个和9 427个差异表达基因。KEGG富集结果显示,温度胁迫严重干扰种子萌发过程中的碳氮平衡,破坏种子萌发所需的能量和物质供应。植物激素信号转导途径呈现温度特异性响应模式,低温(LT)同时干扰赤霉素代谢和脱落酸代谢,而高温(HT)仅影响脱落酸途径。在温度胁迫下,水稻萌发种子茉莉酸信号通路和水杨酸信号通路相关基因的表达量发生显著变化,说明温度胁迫可激活水稻种子的免疫防御反应。低温能显著诱导抗坏血酸代谢与醛糖酸代谢,而高温能促进萜类骨架及泛醌与其他萜类醌的生物合成。多个重要转录因子家族成员表达量发生显著改变,这些转录因子可通过调控下游应激基因表达来增强种子耐逆性。本研究结果系统揭示了水稻种子响应温度胁迫的转录调控网络,对培育广适性水稻品种具有重要意义。
Abstract:
Temperature is an important factor affecting rice seed germination and yield formation. A germination experiment was conducted under three temperature treatments, including normal temperature (NT, 25 ℃), low temperature (LT, 15 ℃), and high temperature (HT, 37 ℃) with Nipponbare as the test material in this study, and rice seeds cultured in dark for 60 h were collected for transcriptome analysis. The results showed that compared with normal temperature treatment, low temperature treatment could significantly delay seed germination, while high temperature treatment accele-rated seed germination. A total of 8 010, 3 929 and 9 427 differentially expressed genes were identified in the low temperature and normal temperature comparison group, the normal temperature and high temperature comparison group, and the low temperature and high temperature comparison group, respectively. Results of KEGG enrichment analysis showed that temperature stress seriously disturbed the carbon and nitrogen balance during seed germination and destroyed the energy and material supply required for seed germination. The plant hormone signal transduction pathway showed a temperature-specific response pattern. Low temperature (LT) interfered with gibberellin metabolism and abscisic acid metabolism, while high temperature (HT) only affected the abscisic acid pathway. Under temperature stress, the expression levels of genes related to jasmonic acid signaling pathway and salicylic acid signaling pathway in germinated rice seeds changed significantly, indicating that temperature stress could activate the immune defensive response of rice seeds. Low temperature could significantly induce ascorbic acid metabolism and aldonic acid metabolism, while high temperature could promote the biosynthesis of terpenoid skeleton and ubiquinone and other terpenoid quinones. The expression levels of several important transcription factor family members changed significantly. These transcription factors can enhance seed stress tolerance by regulating the expression of downstream stress genes. The results of this study systematically revealed the transcriptional regulatory network of rice seeds in response to temperature stress, which are of great significance for cultivating wide-adapted rice varieties.

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备注/Memo

备注/Memo:
收稿日期：2025-04-18基金项目：江苏农林职业技术学院高层次人才科研启动项目(2024rc06)；国家自然科学基金项目(32402663)；中国博士后科学基金第74批面上资助项目(2023M741736)作者简介：李康宁(1995-),女,河北邢台人,博士,讲师,研究方向为植物生理学。(Tel)15295570231;(E-mail)likangningjsnl@163.com。徐凌璐为共同第一作者。
更新日期/Last Update: 2026-04-17