[1]龙宇,胡丽,黎标,等.红螯螯虾室内养殖不同生长阶段适宜水质的水体细菌群落结构及多样性分析[J].江苏农业学报,2025,(07):1375-1387.[doi:doi:10.3969/j.issn.1000-4440.2025.07.014]
　LONG Yu,HU Li,LI Biao,et al.Analysis of the bacterial community structure and diversity in water suitable for indoor aquaculture of Cherax quadricarinatus at different growth stages[J].,2025,(07):1375-1387.[doi:doi:10.3969/j.issn.1000-4440.2025.07.014]
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红螯螯虾室内养殖不同生长阶段适宜水质的水体细菌群落结构及多样性分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年07期
页码:
1375-1387
栏目:
畜牧兽医·水产养殖·益虫饲养
出版日期:
2025-07-31

文章信息/Info

Title:
Analysis of the bacterial community structure and diversity in water suitable for indoor aquaculture of Cherax quadricarinatus at different growth stages
作者:
龙宇1; 胡丽2; 黎标2; 吴亮3; 黄水娥4; 杨海君1
(1.湖南农业大学资源学院,湖南长沙410128；2.湖南中拓环境工程有限公司,湖南长沙410000；3.湖南省分析测试中心有限公司,湖南长沙410131；4.长沙排水有限责任公司,湖南长沙410000)
Author(s):
LONG Yu1; HU Li2; LI Biao2; WU Liang3; HUANG Shui’e4; YANG Haijun1
(1.College of Resources, Hunan Agricultural University, Changsha 410128, China；2.Hunan Zhongtuo Environmental Engineering Co., Ltd., Changsha 410000, China；3.Hunan Analysis and Test Center Co., Ltd., Changsha 410131, China；4.Changsha Drainage Co., Ltd., Changsha 410000, China)
关键词:
红螯螯虾; 水质; 环境微生物; 细菌群落
Keywords:
Cherax quadricarinatus; water quality; environmental microbiota; bacterial community
分类号:
S917.1
DOI:
doi:10.3969/j.issn.1000-4440.2025.07.014
文献标志码:
A
摘要:
为探究红螯螯虾(Cherax quadricarinatus)室内养殖不同生长阶段适宜水环境下的细菌群落特征,本研究以广东省揭阳市南区红螯螯虾室内养殖基地的虾苗(XMP)池、半成品虾(BCPC)池、成品虾(CPP)池1和成品虾(CPP)池2适宜水质的水体为研究对象,通过Illumina MiSeq高通量测序法分析了水体中细菌群落结构、多样性及其与水体理化因子的关联性。结果表明,水体中pH与水温(WT)之间显著正相关(P<0.05),总硬度(TH)与水温、pH之间为显著负相关(P<0.05)。水体中细菌群落优势菌门为变形菌门、拟杆菌门和髌骨细菌门等,优势菌属为多核杆菌属、norank_Rhizobiales_Incertae_Sedis、norank_Chloroplast和hgcl_clade等。通过皮尔逊(Pearson)相关性分析发现,细菌群落多样性与丰富度均受亚硝酸盐氮(NO-2-N)含量显著影响。在属水平上,相对丰度前20的属细菌与水体理化因子的斯皮尔曼（Spearman）相关性分析发现,水体pH、总硬度、氨态氮(NH+4-N)含量、亚硝酸盐氮含量、总氮(TN)含量和化学需氧量对细菌群落结构具有显著影响。利用Tax4Fun2进行基因功能预测,结果表明主要优势功能基因为全球与概览图、碳水化合物代谢和氨基酸代谢等。聚类分析发现相邻生长阶段的细菌群落功能基因更为相似,成品虾池水体中细菌群落功能基因也更加稳定。采用FAPROTAX注释得出,氮代谢循环功能类群的相对丰度在成品虾生长阶段明显增加。本研究结果显示,红螯螯虾室内养殖水体中的菌群结构随生长时期的推进分布更均匀,相关细菌在维持养殖水体健康中发挥了重要作用。
Abstract:
To investigate the bacterial community characteristics in water environments suitable for indoor Cherax quadricarinatus culture at different growth stages, this study focused on the water quality of the shrimp seedling (XMP) pool, semi-finished shrimp (BCPC) pool, finished shrimp (CPP) pool 1, and finished shrimp (CPP) pool 2 at a breeding base in Jieyang, Guangdong. Using Illumina MiSeq high-throughput sequencing, we analyzed the bacterial community structure, diversity, and their association with physicochemical factors in water samples. The results showed that pH was significantly positively correlated with water temperature (WT) (P<0.05), while total hardness (TH) was significantly negatively correlated with WT and pH (P<0.05). The dominant bacterial phyla in the water samples were Proteobacteria, Bacteroidota, and Patescibacteria, while dominant genera included Polynucleobacter, norank_Rhizobiales_Incertae_Sedis, norank_Chloroplast, and hgcI_clade. Pearson correlation analysis revealed that bacterial community diversity and richness were significantly influenced by nitrite-nitrogen (NO-2-N) concentration. At the genus level, Spearman correlation analysis of the top 20 genera showed that pH, TH, ammonia nitrogen (NH+4-N) concentration, NO-2-N concentration, total nitrogen (TN) concentration, and chemical oxygen demand significantly impacted the bacterial community structure. Gene function prediction using Tax4Fun indicated that the main functional pathways included global and overview maps, carbohydrate metabolism, and amino acid metabolism. Clustering analysis demonstrated that the functional genes of bacterial communities at adjacent growth stages were more similar, and the functional genes in finished shrimp pool samples were also more stable. FAPROTAX annotation revealed that the relative abundance of functional groups involved in the nitrogen-metabolism cycle significantly increased during the growth stage of finished shrimp. The research results indicate that, in indoor aquaculture of Cherax quadricarinatus, the bacterial community structure in water becomes more evenly distributed as the growth stage progresses. The relevant bacteria play a crucial role in maintaining the water quality of the aquaculture environment.

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备注/Memo

备注/Memo:
收稿日期：2024-10-29基金项目：湖南省自然科学基金-省市联合项目(2022JJ50247)；长沙市自然科学基金项目(kg2208137)作者简介：龙宇(1999-),女,湖南永州人,硕士研究生,研究方向为农业资源利用。(E-mail)285984550@qq.com通讯作者：杨海君,(E-mail)yhj@hunau.edu.cn
更新日期/Last Update: 2025-08-19