[1]王馨磊,朱思琪,田晓娜,等.紫花苜蓿RALF基因家族的全基因组鉴定及盐胁迫下表达分析[J].江苏农业学报,2025,(07):1260-1269.[doi:doi:10.3969/j.issn.1000-4440.2025.07.002]
　WANG Xinlei,ZHU Siqi,TIAN Xiaona,et al.Genome-wide identification of the RALF gene family in Medicago sativa and expression analysis under salt stress[J].,2025,(07):1260-1269.[doi:doi:10.3969/j.issn.1000-4440.2025.07.002]
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紫花苜蓿RALF基因家族的全基因组鉴定及盐胁迫下表达分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年07期
页码:
1260-1269
栏目:
遗传育种·生理生化
出版日期:
2025-07-31

文章信息/Info

Title:
Genome-wide identification of the RALF gene family in Medicago sativa and expression analysis under salt stress
作者:
王馨磊; 朱思琪; 田晓娜; 杨杞
(内蒙古农业大学旱寒区植物逆境适应与遗传修饰改良自治区重点实验室, 内蒙古呼和浩特010018)
Author(s):
WANG Xinlei; ZHU Siqi; TIAN Xiaona; YANG Qi
(Key Laboratory of Plants Adversity Adaptation and Genetic Improvement in Cold and Arid Regions of Inner Mongolia, Inner Mongolia Agricultural University, Hohhot 010018, China)
关键词:
紫花苜蓿; RALF基因; 表达分析; 生物信息学分析; 盐胁迫
Keywords:
Medicago sativa; RALF gene; expression analysis; bioinformatics; salt stress
分类号:
S68；Q946.83+6
DOI:
doi:10.3969/j.issn.1000-4440.2025.07.002
文献标志码:
A
摘要:
快速碱化因子RALF是一类在植物生长发育及胁迫响应中发挥重要作用的小分子信号肽。为探讨盐胁迫条件下RALF基因家族中的关键基因,本研究对紫花苜蓿全基因组进行了RALF基因家族成员的鉴定,并分析了其盐胁迫响应特征。通过全基因组BLAST分析,鉴定出紫花苜蓿有9个RALF基因,编码的蛋白质氨基酸序列长度从59 aa到128 aa,等电点为5.13～5.41,均为疏水性蛋白。这些基因分布于6条染色体上,其中2对基因表现出共线性关系。系统发育分析将紫花苜蓿中9个RALF基因分为4个组,且所有基因编码的蛋白质均保留了motif1和motif2保守基序。此外,启动子分析结果显示大多数MsRALF基因具有响应植物生长发育及胁迫的顺式作用元件。盐胁迫处理7 d结果显示,MsRALF1、MsRALF2、MsRALF3的表达量呈先下降后上升的趋势,并在第7 d达到峰值；MsRALF6的表达量呈先下降后上升再下降的趋势,并在第3 d达到峰值；MsRALF4、MsRALF5、MsRALF7、MsRALF8、MsRALF9的表达量均在盐胁迫当天达峰值。本研究结果表明,紫花苜蓿中鉴定出的9个MsRALF基因可能参与了植物对盐胁迫的响应,且其表达受到盐胁迫调控。
Abstract:
Rapid alkalinization factor (RALF) is a class of small peptides that play crucial roles in plant growth, development, and stress response. To investigate key genes involved in salt stress response, we identified members of the RALF gene family in the whole genome of Medicago sativa and analyzed their response characteristics under salt stress. Through whole-genome BLAST analysis, we identified nine RALF genes in M. sativa. The encoded proteins ranged from 59 to 128 amino acids in length. Their isoelectric points clustered between 5.13 and 5.41. All exhibited hydrophobic properties. These genes were distributed across six chromosomes, with two pairs exhibiting collinearity.Phylogenetic analysis grouped these genes into four subgroups, and all members retained the conserved motif1 and motif2. Furthermore, promoter analysis revealed that most MsRALF genes contained cis-regulatory elements associated with plant growth, development, and stress response. Under salt stress, MsRALF1, MsRALF2 and MsRALF3 expression initially decreased but subsequently increased, peaking at 7 d, while MsRALF6 declined, then increased, and finally decreased, reaching maximal expression at 3 d. MsRALF4, MsRALF5, MsRALF7, MsRALF8 and MsRALF9 all peaked immediately on day 0 of salt exposure. These findings suggest that the nine MsRALF genes identified in M. sativa may be involved in the plant’s response to salt stress, with their expression being regulated by salt stress conditions.

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备注/Memo

备注/Memo:
收稿日期:2024-11-27基金项目:内蒙古自然科学基金项目(2022MS03034)；国家自然科学基金项目(32060066)；内蒙古自治区植物逆境生理与分子生物学重点实验室能力建设项目(BR221021)作者简介:王馨磊(1999-),男,内蒙古通辽人,硕士,研究方向为植物分子生物学。(E-mail)3041706586@qq.com通讯作者:杨杞,(E-mail)atp_yangqi@imau.edu.cn
更新日期/Last Update: 2025-08-19