[1]费思恬,侯鹰翔,宋松泉,等.水稻GT家族成员鉴定及响应非生物胁迫的表达分析[J].江苏农业学报,2025,(03):417-431.[doi:doi:10.3969/j.issn.1000-4440.2025.03.001]
　FEI Sitian,HOU Yingxiang,SONG Songquan,et al.Identification of GT family members in rice and expression analysis in response to abiotic stresses[J].,2025,(03):417-431.[doi:doi:10.3969/j.issn.1000-4440.2025.03.001]
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水稻GT家族成员鉴定及响应非生物胁迫的表达分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年03期
页码:
417-431
栏目:
遗传育种·生理生化
出版日期:
2025-03-31

文章信息/Info

Title:
Identification of GT family members in rice and expression analysis in response to abiotic stresses
作者:
费思恬1; 2; 3; 侯鹰翔1; 2; 宋松泉1; 罗勇2; 张超1; 2
(1.湘南学院南岭现代种业研究院,湖南郴州423000；2.湘南学院化学与环境科学学院,湖南郴州423000；3.湘南学院郴州市汝城小黄姜繁育技术研发中心,湖南郴州423000)
Author(s):
FEI Sitian1; 2; 3; HOU Yingxiang1; 2; SONG Songquan1; LUO Yong2; ZHANG Chao1; 2
(1.Nanling Research Institute for Modern Seed Industry, Xiangnan University, Chenzhou 423000, China;2.College of Chemistry and Environmental Science, Xiangnan University, Chenzhou 423000, China;3.R&D Center for Breeding Technology of Rucheng Small Yellow Ginger in Chenzhou City, Xiangnan University, Chenzhou 423000, China)
关键词:
水稻; GT家族; 生物信息学; 基因表达; 非生物胁迫
Keywords:
rice; GT family; bioinformatics; gene expression; abiotic stress
分类号:
S511
DOI:
doi:10.3969/j.issn.1000-4440.2025.03.001
文献标志码:
A
摘要:
GT家族是植物中一类重要的转录因子,在植物生长发育以及逆境响应中发挥重要作用。本研究结合生物信息学和转录组学对该家族成员、蛋白质理化性质、进化关系以及非生物胁迫响应模式进行分析。结果表明,水稻基因组中共有52个GT基因,这些基因分布在12条染色体上。基于系统进化分析,GT蛋白家族可分为3个亚家族。大多数GT基因在水稻叶片、叶鞘、根和茎等营养器官中的相对表达量较低,而在花序、胚和胚乳等生殖器官中的相对表达量较高。相较于油菜素内酯(BR)、细胞分裂素(CTK)和赤霉素(GA),GT基因对脱落酸(ABA)、茉莉酸(JA)和生长素(IAA)的响应更为显著,表明GT家族成员可能参与ABA、JA和IAA介导的植物逆境响应过程。在光周期下,OsGT2、OsGT18、OsGT17、OsGT25和OsGT10的相对表达量呈现昼降夜升的表达模式,OsGT15、OsGT34和OsGT16的相对表达量呈现昼升夜降的表达模式。这些基因的表达可能受到光信号的调控。盐胁迫下,OsGT2在耐盐品种盐稻3931和广湘24S中的相对表达量上升,在不耐盐品种19H216和晶4155S中的相对表达量下降。水淹胁迫下,OsGT51、OsGT48在耐水淹品种LS273根系中的相对表达量下降,在不耐水淹品种ZZ39根系中的相对表达量与对照相比无显著差异。表明OsGT51、OsGT48可能参与了水稻对水淹胁迫的响应过程。随着高温胁迫、干旱胁迫和重金属镉胁迫时间的延长,中花11大多数GT基因的相对表达量下降,仅有少数GT基因的相对表达量上升。本研究结果为深入解析GT转录因子家族在水稻生长发育和逆境响应中的分子机制提供了重要依据。
Abstract:
The GT family is an important class of transcription factors in plants, playing a crucial role in plant growth, development, and stress responses. In this study, bioinformatics and transcriptomics were combined to analyze the protein physicochemical properties, evolutionary relationships, and abiotic stress response patterns of the family members. The results showed that there were 52 GT genes in the rice genome, which were distributed on 12 chromosomes. Based on phylogenetic analysis, the GT protein family could be divided into three subfamilies. The relative expression levels of most GT genes were low in vegetative organs such as rice leaves, leaf sheaths, roots, and stems. In contrast, relatively high expression levels were observed in reproductive organs such as inflorescences, embryos, and endosperms. Compared with brassinosteroid (BR), cytokinin (CTK), and gibberellin (GA), GT genes responded more significantly to abscisic acid (ABA), jasmonic acid (JA), and auxin (IAA), indicating that GT family members may be involved in the plant stress response processes mediated by ABA, JA, and IAA. Under photoperiod conditions, the relative expression levels of OsGT2, OsGT18, OsGT17, OsGT25, and OsGT10 decreased during the day and increased at night, while those of OsGT15, OsGT34, and OsGT16 increased during the day and decreased at night. The expression of these genes may be regulated by light signals. Under salt stress, the relative expression level of OsGT2 increased in the salt-tolerant varieties Yandao 3931 and Guangxiang 24S, but decreased in the salt-sensitive varieties 19H216 and Jing 4155S. Under waterlogging stress, the relative expression levels of OsGT51 and OsGT48 decreased in the roots of the waterlogging-tolerant variety LS273, while the relative expression levels in the roots of the waterlogging-sensitive variety ZZ39 were not significantly different from the control. This indicated that OsGT51 and OsGT48 may be involved in the response of rice to waterlogging stress. With the prolongation of high-temperature stress, drought stress, and heavy metal cadmium stress, the relative expression levels of most GT genes in Zhonghua 11 decreased, while only a few GT genes showed an increase. The results of this study provide an important basis for in-depth analysis of the molecular mechanisms of the GT transcription factor family in rice growth, development, and stress responses.

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备注/Memo

备注/Memo:
收稿日期：2024-07-25基金项目：湖南省教育厅科学研究项目(22C0548)；郴州国家可持续发展议程创新示范区建设省级专项(2022sfq06)；湖南省科技创新计划资助项目(2023RC3190)；湘南学院校级科研项目青年项目(2023XJ33)作者简介：费思恬(1997-),女,湖南郴州人,硕士,助理实验师,主要从事植物分子生物学研究。(E-mail)924770209@qq.com通讯作者：张超,(E-mail)KingofZC@126.com
更新日期/Last Update: 2025-04-27